PRIMER PICKING RESULTS FOR NM_005157,Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1), transcript variant a, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        478   20   59.99   60.00  7.00  0.00 11.00 GCCTCAGGGTCTGAGTGAAG
RIGHT PRIMER       910   20   59.99   50.00  3.00  1.00 12.00 GCCATCAGAAGCAGTGTTGA
SEQUENCE SIZE: 5744
INCLUDED REGION SIZE: 5744

PRODUCT SIZE: 433, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        479   20   59.99   60.00  7.00  2.00 10.00 CCTCAGGGTCTGAGTGAAGC
   RIGHT PRIMER       910   20   59.99   50.00  3.00  1.00 12.00 GCCATCAGAAGCAGTGTTGA
   PRODUCT SIZE: 432, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER        477   20   59.99   55.00  7.00  1.00 10.00 AGCCTCAGGGTCTGAGTGAA
   RIGHT PRIMER       910   20   59.99   50.00  3.00  1.00 12.00 GCCATCAGAAGCAGTGTTGA
   PRODUCT SIZE: 434, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       3710   20   59.99   50.00  4.00  3.00 10.00 AAGCTCCTCAGTTCGGTGAA
   RIGHT PRIMER      3838   20   59.98   55.00  5.00  2.00 11.00 CAGCCACTGTCATGGGTATG
   PRODUCT SIZE: 129, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       4387   20   59.97   50.00  2.00  1.00 11.00 GGTGGAAAACAGGGTGCTAA
   RIGHT PRIMER      5078   20   60.01   50.00  4.00  2.00 12.00 CAGGCGTGCTCTGTGAAATA
   PRODUCT SIZE: 692, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00