PRIMER PICKING RESULTS FOR NM_005211,Homo sapiens colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog (CSF1R), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2137   20   59.99   50.00  2.00  1.00 12.00 GTGGCTGTGAAGATGCTGAA
RIGHT PRIMER      2335   20   59.99   50.00  8.00  2.00 11.00 CCTTCCTTCGCAGAAAGTTG
SEQUENCE SIZE: 3992
INCLUDED REGION SIZE: 3992

PRODUCT SIZE: 199, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       2137   20   59.99   50.00  2.00  1.00 12.00 GTGGCTGTGAAGATGCTGAA
   RIGHT PRIMER      2496   20   59.99   55.00  6.00  2.00 12.00 AGAAGTGGAGACAGGCCTCA
   PRODUCT SIZE: 360, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       1386   20   59.99   60.00  2.00  2.00 12.00 CACCTTCACCCTCTCTCTGC
   RIGHT PRIMER      2163   20   59.98   50.00  3.00  1.00 11.00 CGTGGACTTCAGCATCTTCA
   PRODUCT SIZE: 778, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        180   20   59.99   60.00  4.00  0.00 12.00 CCAGTGCAGAGGAGAGGAAC
   RIGHT PRIMER      1042   20   59.98   55.00  4.00  0.00  9.00 GAGGGATTGCGAGCTTAGTG
   PRODUCT SIZE: 863, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1024   20   59.98   50.00  4.00  1.00 10.00 ACTAAGCTCGCAATCCCTCA
   RIGHT PRIMER      1405   20   59.99   60.00  2.00  0.00 12.00 GCAGAGAGAGGGTGAAGGTG
   PRODUCT SIZE: 382, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00