PRIMER PICKING RESULTS FOR NM_005228,Homo sapiens epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        355   20   60.00   55.00  3.00  2.00 11.00 AACTGTGAGGTGGTCCTTGG
RIGHT PRIMER      1056   20   60.00   50.00  7.00  2.00 11.00 GGTGGCACCAAAGCTGTATT
SEQUENCE SIZE: 5532
INCLUDED REGION SIZE: 5532

PRODUCT SIZE: 702, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       4669   20   60.01   40.00  4.00  0.00 11.00 TGGATTCATCAGCATTTGGA
   RIGHT PRIMER      5072   20   60.00   50.00  3.00  1.00 11.00 GCACCTGTAAAATGCCCTGT
   PRODUCT SIZE: 404, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER        433   20   59.99   55.00  3.00  0.00 10.00 CAGGAGGTGGCTGGTTATGT
   RIGHT PRIMER      1056   20   60.00   50.00  7.00  2.00 11.00 GGTGGCACCAAAGCTGTATT
   PRODUCT SIZE: 624, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER        787   20   59.99   60.00  4.00  1.00 10.00 GGTGCAGGAGAGGAGAACTG
   RIGHT PRIMER      1056   20   60.00   50.00  7.00  2.00 11.00 GGTGGCACCAAAGCTGTATT
   PRODUCT SIZE: 270, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       3112   20   60.01   50.00  6.00  1.00  9.00 CAGCGCTACCTTGTCATTCA
   RIGHT PRIMER      3306   20   60.00   55.00  7.00  2.00 11.00 TGCACTCAGAGAGCTCAGGA
   PRODUCT SIZE: 195, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00