PRIMER PICKING RESULTS FOR NM_005232,Homo sapiens EphA1 (EPHA1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        880   20   60.01   55.00  7.00  3.00 11.00 CACTGTGAGCCTGGCTATGA
RIGHT PRIMER      1688   20   60.00   50.00  6.00  0.00 10.00 TCATGATCAGGGGAGAAAGG
SEQUENCE SIZE: 3370
INCLUDED REGION SIZE: 3370

PRODUCT SIZE: 809, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       1614   20   59.99   55.00  3.00  2.00 10.00 GCCTGACACCACATACATCG
   RIGHT PRIMER      2171   20   59.98   50.00  4.00  1.00 10.00 ATATGCGGGTGGCTAAACTG
   PRODUCT SIZE: 558, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1614   20   59.99   55.00  3.00  2.00 10.00 GCCTGACACCACATACATCG
   RIGHT PRIMER      2561   20   59.97   50.00  4.00  0.00 11.00 ACAATCCCAAAGCTCCACAC
   PRODUCT SIZE: 948, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1614   20   59.99   55.00  3.00  2.00 10.00 GCCTGACACCACATACATCG
   RIGHT PRIMER      2608   20   60.03   50.00  5.00  3.00 11.00 TCATCTCCCCATAAGGCTTG
   PRODUCT SIZE: 995, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1614   20   59.99   55.00  3.00  2.00 10.00 GCCTGACACCACATACATCG
   RIGHT PRIMER      2156   20   59.96   50.00  5.00  2.00 10.00 AACTGGCCCATGATAGTTGC
   PRODUCT SIZE: 543, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00