PRIMER PICKING RESULTS FOR NM_005233,Homo sapiens EphA3 (EPHA3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER         21   20   60.02   60.00  4.00  2.00 11.00 GCTGAAGACGGCACTAGGAC
RIGHT PRIMER       994   20   60.00   50.00  5.00  2.00 10.00 ACTTGCCAATGGGTACAAGC
SEQUENCE SIZE: 5799
INCLUDED REGION SIZE: 5799

PRODUCT SIZE: 974, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       2259   20   60.01   50.00  4.00  2.00 10.00 CCACCCCAATATCATTCGAC
   RIGHT PRIMER      2587   20   60.01   50.00  5.00  0.00 12.00 TTGGGATCTTCCCTCCTCTT
   PRODUCT SIZE: 329, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER        795   20   60.02   55.00  3.00  3.00 11.00 TGCCTTGGTGTCTGTGAGAG
   RIGHT PRIMER       994   20   60.00   50.00  5.00  2.00 10.00 ACTTGCCAATGGGTACAAGC
   PRODUCT SIZE: 200, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        975   20   60.00   50.00  5.00  3.00 11.00 GCTTGTACCCATTGGCAAGT
   RIGHT PRIMER      1428   20   60.02   55.00  7.00  3.00 12.00 TGCCAGAAGGTCTGTCACTG
   PRODUCT SIZE: 454, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER        216   20   59.97   50.00  4.00  1.00 11.00 CACCAGCAACATGGATTGTC
   RIGHT PRIMER       994   20   60.00   50.00  5.00  2.00 10.00 ACTTGCCAATGGGTACAAGC
   PRODUCT SIZE: 779, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00