PRIMER PICKING RESULTS FOR NM_005235,Homo sapiens v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (ERBB4), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       3989   20   59.97   50.00  4.00  0.00 10.00 ACACACCTGCTCCAATTTCC
RIGHT PRIMER      4886   20   59.99   50.00  4.00  1.00 10.00 CAACCAACGGCCTATCAAGT
SEQUENCE SIZE: 5484
INCLUDED REGION SIZE: 5484

PRODUCT SIZE: 898, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       3989   20   59.97   50.00  4.00  0.00 10.00 ACACACCTGCTCCAATTTCC
   RIGHT PRIMER      4887   20   59.99   50.00  4.00  1.00  9.00 ACAACCAACGGCCTATCAAG
   PRODUCT SIZE: 899, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       3656   20   60.01   50.00  5.00  3.00 11.00 ACCTCAACACCTTTGCCAAC
   RIGHT PRIMER      4353   20   59.97   50.00  5.00  2.00 10.00 GGGTGTTTCAACCATCTGCT
   PRODUCT SIZE: 698, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       4340   20   59.96   45.00  5.00  2.00 11.00 TGGTTGAAACACCCATGCTA
   RIGHT PRIMER      4886   20   59.99   50.00  4.00  1.00 10.00 CAACCAACGGCCTATCAAGT
   PRODUCT SIZE: 547, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       4340   20   59.96   45.00  5.00  2.00 11.00 TGGTTGAAACACCCATGCTA
   RIGHT PRIMER      4887   20   59.99   50.00  4.00  1.00  9.00 ACAACCAACGGCCTATCAAG
   PRODUCT SIZE: 548, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00