PRIMER PICKING RESULTS FOR NM_005246,Homo sapiens fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) (FER), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1699   20   60.00   55.00  3.00  0.00 10.00 GTGGGCTCTTCAGCACTTTC
RIGHT PRIMER      1843   20   59.98   50.00  4.00  0.00 11.00 CATGACTCTCTCGCACCAAA
SEQUENCE SIZE: 2950
INCLUDED REGION SIZE: 2950

PRODUCT SIZE: 145, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        158   20   59.97   55.00  6.00  3.00 12.00 CGCATTCTAGACTCCCGAAG
   RIGHT PRIMER       397   20   60.02   40.00  4.00  2.00 11.00 TCCCAAACCCCATTTTGTAA
   PRODUCT SIZE: 240, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER        265   20   59.96   55.00  5.00  2.00 10.00 GACACCTCATCATGGACACG
   RIGHT PRIMER       397   20   60.02   40.00  4.00  2.00 11.00 TCCCAAACCCCATTTTGTAA
   PRODUCT SIZE: 133, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER        958   20   59.95   50.00  4.00  1.00 12.00 GCACAGCTCCATCAGAATCA
   RIGHT PRIMER      1843   20   59.98   50.00  4.00  0.00 11.00 CATGACTCTCTCGCACCAAA
   PRODUCT SIZE: 886, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1183   20   60.05   50.00  3.00  1.00 12.00 CACAGAACAACGGCTGCTAA
   RIGHT PRIMER      1843   20   59.98   50.00  4.00  0.00 11.00 CATGACTCTCTCGCACCAAA
   PRODUCT SIZE: 661, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00