PRIMER PICKING RESULTS FOR NM_005248,Homo sapiens Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        760   20   59.98   55.00  3.00  0.00 10.00 ATGGGCGGCTACTACATCAC
RIGHT PRIMER      1392   20   60.00   55.00  4.00  0.00 12.00 GCAGGGGTTGTACTCATCGT
SEQUENCE SIZE: 2354
INCLUDED REGION SIZE: 2354

PRODUCT SIZE: 633, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        758   20   59.98   55.00  4.00  0.00 10.00 ACATGGGCGGCTACTACATC
   RIGHT PRIMER      1392   20   60.00   55.00  4.00  0.00 12.00 GCAGGGGTTGTACTCATCGT
   PRODUCT SIZE: 635, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER        757   20   59.98   55.00  4.00  2.00  9.00 GACATGGGCGGCTACTACAT
   RIGHT PRIMER      1392   20   60.00   55.00  4.00  0.00 12.00 GCAGGGGTTGTACTCATCGT
   PRODUCT SIZE: 636, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER        788   20   60.02   50.00  7.00  2.00 11.00 TTCAGTTCAACTCGGTGCAG
   RIGHT PRIMER      1392   20   60.00   55.00  4.00  0.00 12.00 GCAGGGGTTGTACTCATCGT
   PRODUCT SIZE: 605, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER         13   20   60.03   50.00  7.00  0.00 10.00 ATCTAGGCCCAAGAGGGAAA
   RIGHT PRIMER       692   20   59.99   55.00  4.00  3.00 10.00 AGGGAGTAGGCACCTTTGGT
   PRODUCT SIZE: 680, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00