PRIMER PICKING RESULTS FOR NM_005356,Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK),mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1183   20   59.99   45.00  8.00  2.00 12.00 TGCAAGATTGCAGACTTTGG
RIGHT PRIMER      1581   20   60.01   55.00  5.00  2.00 12.00 TCAAGGCTGAGGCTGGTACT
SEQUENCE SIZE: 2032
INCLUDED REGION SIZE: 2032

PRODUCT SIZE: 399, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       1366   20   60.01   55.00  7.00  1.00 10.50 ATCCCTTACCCAGGGATGAC
   RIGHT PRIMER      1581   20   60.01   55.00  5.00  2.00 12.00 TCAAGGCTGAGGCTGGTACT
   PRODUCT SIZE: 216, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1183   20   59.99   45.00  8.00  2.00 12.00 TGCAAGATTGCAGACTTTGG
   RIGHT PRIMER      1872   20   59.95   60.00  2.00  0.00 11.00 GCTTCTCCCCTCTCTCTGGT
   PRODUCT SIZE: 690, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       1562   20   60.01   55.00  5.00  3.00 12.00 AGTACCAGCCTCAGCCTTGA
   RIGHT PRIMER      1872   20   59.95   60.00  2.00  0.00 11.00 GCTTCTCCCCTCTCTCTGGT
   PRODUCT SIZE: 311, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1366   20   60.01   55.00  7.00  1.00 10.50 ATCCCTTACCCAGGGATGAC
   RIGHT PRIMER      1872   20   59.95   60.00  2.00  0.00 11.00 GCTTCTCCCCTCTCTCTGGT
   PRODUCT SIZE: 507, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00