PRIMER PICKING RESULTS FOR NM_005417,Homo sapiens v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1917   20   59.99   60.00  2.00  1.00 11.00 GCTCTGTGGGTCTCTGGAAG
RIGHT PRIMER      2651   20   60.00   55.00  3.00  1.00 11.00 GCTGGCTCTGAAGGTCAAAC
SEQUENCE SIZE: 3711
INCLUDED REGION SIZE: 3711

PRODUCT SIZE: 735, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       1916   20   59.99   55.00  4.00  0.00 11.00 AGCTCTGTGGGTCTCTGGAA
   RIGHT PRIMER      2651   20   60.00   55.00  3.00  1.00 11.00 GCTGGCTCTGAAGGTCAAAC
   PRODUCT SIZE: 736, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1733   20   59.99   60.00  3.00  0.00 11.00 CCTGCTGTTGGTCCTCTCTC
   RIGHT PRIMER      2651   20   60.00   55.00  3.00  1.00 11.00 GCTGGCTCTGAAGGTCAAAC
   PRODUCT SIZE: 919, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER        226   20   59.99   55.00  5.00  1.00 11.00 CTGTTCGGAGGCTTCAACTC
   RIGHT PRIMER       609   20   60.01   55.00  4.00  2.00 11.00 TGAGAGGCAGTAGGCACCTT
   PRODUCT SIZE: 384, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER         42   20   60.02   55.00  4.00  2.00 11.00 GGGTAGCAACAAGAGCAAGC
   RIGHT PRIMER       245   20   59.99   55.00  5.00  1.00 11.00 GAGTTGAAGCCTCCGAACAG
   PRODUCT SIZE: 204, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00