PRIMER PICKING RESULTS FOR NM_005424,Homo sapiens tyrosine kinase with immunoglobulin and epidermal growth factor homology domains (TIE), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2844   20   60.00   55.00  4.00  0.00 12.00 GACTGACCCAGCTTTTGCTC
RIGHT PRIMER      3527   20   60.01   60.00  5.00  3.00 10.00 GGGTCAGACTGGTCACAGGT
SEQUENCE SIZE: 3845
INCLUDED REGION SIZE: 3845

PRODUCT SIZE: 684, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       2842   20   60.00   55.00  4.00  2.00 10.00 GAGACTGACCCAGCTTTTGC
   RIGHT PRIMER      3527   20   60.01   60.00  5.00  3.00 10.00 GGGTCAGACTGGTCACAGGT
   PRODUCT SIZE: 686, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2122   20   59.99   60.00  8.00  0.00 12.00 GACAGGCCTGAGGAGACAAG
   RIGHT PRIMER      2863   20   60.00   55.00  4.00  1.00 10.00 GAGCAAAAGCTGGGTCAGTC
   PRODUCT SIZE: 742, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       2122   20   59.99   60.00  8.00  0.00 12.00 GACAGGCCTGAGGAGACAAG
   RIGHT PRIMER      2861   20   60.00   55.00  4.00  1.00 10.00 GCAAAAGCTGGGTCAGTCTC
   PRODUCT SIZE: 740, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER        492   20   59.99   55.00  5.00  2.00 10.00 GCAGACAGACGTGATCTGGA
   RIGHT PRIMER       742   20   59.99   55.00  8.00  3.00 10.00 CACCTCCATGTAGGCAACCT
   PRODUCT SIZE: 251, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00