PRIMER PICKING RESULTS FOR NM_005433,Homo sapiens v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1290   20   60.03   45.00  4.00  0.00 11.00 CAGCAAGACAAGGTGCAAAA
RIGHT PRIMER      1927   20   59.99   55.00  5.00  1.00 12.00 AGGAGCCTCACTGTCCTTCA
SEQUENCE SIZE: 4343
INCLUDED REGION SIZE: 4343

PRODUCT SIZE: 638, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       1290   20   60.03   45.00  4.00  0.00 11.00 CAGCAAGACAAGGTGCAAAA
   RIGHT PRIMER      1928   20   59.99   60.00  7.00  0.00 12.00 CAGGAGCCTCACTGTCCTTC
   PRODUCT SIZE: 639, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER        945   20   59.96   45.00  6.00  1.00 10.00 TGATGCCAGAAGCTTTCCTT
   RIGHT PRIMER      1928   20   59.99   60.00  7.00  0.00 12.00 CAGGAGCCTCACTGTCCTTC
   PRODUCT SIZE: 984, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER        947   20   59.96   45.00  6.00  2.00 11.00 ATGCCAGAAGCTTTCCTTCA
   RIGHT PRIMER      1928   20   59.99   60.00  7.00  0.00 12.00 CAGGAGCCTCACTGTCCTTC
   PRODUCT SIZE: 982, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER        947   20   59.96   45.00  6.00  2.00 11.00 ATGCCAGAAGCTTTCCTTCA
   RIGHT PRIMER      1927   20   59.99   55.00  5.00  1.00 12.00 AGGAGCCTCACTGTCCTTCA
   PRODUCT SIZE: 981, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00