PRIMER PICKING RESULTS FOR NM_005592,Homo sapiens muscle, skeletal, receptor tyrosine kinase (MUSK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1009   20   59.99   55.00  4.00  2.00 12.00 AGGGGAGGTGTGTAATGCAG
RIGHT PRIMER      1671   20   60.02   55.00  3.00  0.00 11.00 TCAGAAGGCAGTGTGGTGAG
SEQUENCE SIZE: 2666
INCLUDED REGION SIZE: 2666

PRODUCT SIZE: 663, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1009   20   59.99   55.00  4.00  2.00 12.00 AGGGGAGGTGTGTAATGCAG
   RIGHT PRIMER      1672   20   60.02   55.00  3.00  1.00 12.00 CTCAGAAGGCAGTGTGGTGA
   PRODUCT SIZE: 664, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       1995   20   59.99   45.00  6.00  3.00 10.00 CAATGTGCCTGCTCTTTGAA
   RIGHT PRIMER      2277   20   59.98   45.00  4.00  3.00 10.00 AAGTCGGCAATTTTCACCAC
   PRODUCT SIZE: 283, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER        362   20   59.99   60.00  4.00  0.00 10.00 GGAGGAGCTGTGGAGAGTTG
   RIGHT PRIMER      1098   20   59.98   60.00  4.00  0.00 11.00 AGTAGCTCTTGGGCCTCCTC
   PRODUCT SIZE: 737, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       1652   20   60.02   55.00  3.00  3.00 12.00 CTCACCACACTGCCTTCTGA
   RIGHT PRIMER      2277   20   59.98   45.00  4.00  3.00 10.00 AAGTCGGCAATTTTCACCAC
   PRODUCT SIZE: 626, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00