PRIMER PICKING RESULTS FOR NM_005607,Homo sapiens PTK2 protein tyrosine kinase 2 (PTK2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2941   20   60.01   55.00  4.00  2.00 10.00 ACCAGCCTCCAGCAAGAGTA
RIGHT PRIMER      3864   20   60.00   45.00  3.00  0.00 12.00 ATGGAAAAAGGGGATGGTTC
SEQUENCE SIZE: 4162
INCLUDED REGION SIZE: 4162

PRODUCT SIZE: 924, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       2941   20   60.01   55.00  4.00  2.00 10.00 ACCAGCCTCCAGCAAGAGTA
   RIGHT PRIMER      3867   20   59.99   40.00  2.00  0.00 12.00 AAAATGGAAAAAGGGGATGG
   PRODUCT SIZE: 927, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       2705   20   59.98   50.00  8.00  2.00 12.00 TGGTGAAAGCTGTCATCGAG
   RIGHT PRIMER      2960   20   60.01   55.00  4.00  0.00 10.00 TACTCTTGCTGGAGGCTGGT
   PRODUCT SIZE: 256, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       3302   20   59.97   40.00  5.00  0.00 10.00 CCAAAATGTGGCGTTTTTCT
   RIGHT PRIMER      3864   20   60.00   45.00  3.00  0.00 12.00 ATGGAAAAAGGGGATGGTTC
   PRODUCT SIZE: 563, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       3302   20   59.97   40.00  5.00  0.00 10.00 CCAAAATGTGGCGTTTTTCT
   RIGHT PRIMER      3867   20   59.99   40.00  2.00  0.00 12.00 AAAATGGAAAAAGGGGATGG
   PRODUCT SIZE: 566, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00