PRIMER PICKING RESULTS FOR NM_005781,Homo sapiens activated p21cdc42Hs kinase (ACK1), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2747   20   60.01   55.00  4.00  1.00 11.00 TACAGCAGGAGTGCATGAGG
RIGHT PRIMER      3205   20   60.00   55.00  4.00  2.00 11.00 GCTCGGGCAGCAAGTAATAG
SEQUENCE SIZE: 4548
INCLUDED REGION SIZE: 4548

PRODUCT SIZE: 459, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       1991   20   59.99   50.00  4.00  0.00 11.00 TGCAGAACAGCTTCATCCAC
   RIGHT PRIMER      2766   20   60.01   55.00  4.00  2.00 10.00 CCTCATGCACTCCTGCTGTA
   PRODUCT SIZE: 776, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1992   20   59.99   50.00  4.00  0.00 11.00 GCAGAACAGCTTCATCCACA
   RIGHT PRIMER      2766   20   60.01   55.00  4.00  2.00 10.00 CCTCATGCACTCCTGCTGTA
   PRODUCT SIZE: 775, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER       2556   20   59.95   50.00  4.00  1.00 11.00 GATCTGCTCCATCAACAGCA
   RIGHT PRIMER      3205   20   60.00   55.00  4.00  2.00 11.00 GCTCGGGCAGCAAGTAATAG
   PRODUCT SIZE: 650, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       1123   20   60.08   50.00  6.00  3.00 10.00 GAAGCCATGGACGACTTCAT
   RIGHT PRIMER      1602   20   59.99   50.00  4.00  2.00 10.00 CTGGCCGTAGGTGAACATTT
   PRODUCT SIZE: 480, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00