PRIMER PICKING RESULTS FOR NM_005975,Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2324   20   60.02   60.00  2.00  0.00 10.00 GCGTGTGCTCCTCTCCTTAC
RIGHT PRIMER      2546   20   59.98   55.00  5.00  0.00 12.00 CCAAGCTGAACTGGGAAGAG
SEQUENCE SIZE: 3527
INCLUDED REGION SIZE: 3527

PRODUCT SIZE: 223, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       2063   20   60.02   50.00  4.00  3.00 10.00 ACAAGCTGATGCTGACATGC
   RIGHT PRIMER      2546   20   59.98   55.00  5.00  0.00 12.00 CCAAGCTGAACTGGGAAGAG
   PRODUCT SIZE: 484, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2061   20   60.02   50.00  6.00  3.00 10.00 GCACAAGCTGATGCTGACAT
   RIGHT PRIMER      2546   20   59.98   55.00  5.00  0.00 12.00 CCAAGCTGAACTGGGAAGAG
   PRODUCT SIZE: 486, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 3 LEFT PRIMER       2256   20   59.96   55.00  3.00  1.00 10.00 CCTGGGGTTTACTGAGGTGA
   RIGHT PRIMER      2546   20   59.98   55.00  5.00  0.00 12.00 CCAAGCTGAACTGGGAAGAG
   PRODUCT SIZE: 291, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

 4 LEFT PRIMER       2324   20   60.02   60.00  2.00  0.00 10.00 GCGTGTGCTCCTCTCCTTAC
   RIGHT PRIMER      2620   20   60.04   55.00  3.00  2.00  9.00 GGGAGGAAAGAACCCTTGAG
   PRODUCT SIZE: 297, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00