PRIMER PICKING RESULTS FOR NM_006180,Homo sapiens neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1705   20   60.01   55.00  2.00  1.00 11.00 GGGACACCACGAACAGAAGT
RIGHT PRIMER      2497   20   60.00   50.00  7.00  2.00 10.00 ATATGCAGCATCTGCGACTG
SEQUENCE SIZE: 4057
INCLUDED REGION SIZE: 4057

PRODUCT SIZE: 793, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER       2317   20   60.02   50.00  6.00  2.00 10.00 AGCATGAGCACATCGTCAAG
   RIGHT PRIMER      2497   20   60.00   50.00  7.00  2.00 10.00 ATATGCAGCATCTGCGACTG
   PRODUCT SIZE: 181, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       1705   20   60.01   55.00  2.00  1.00 11.00 GGGACACCACGAACAGAAGT
   RIGHT PRIMER      2224   20   59.99   55.00  8.00  0.00 12.00 TCCTGCTCAGGACAGAGGTT
   PRODUCT SIZE: 520, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1731   20   59.97   55.00  6.00  3.00 10.00 ATCCCTTCCACAGACGTCAC
   RIGHT PRIMER      2497   20   60.00   50.00  7.00  2.00 10.00 ATATGCAGCATCTGCGACTG
   PRODUCT SIZE: 767, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER       1705   20   60.01   55.00  2.00  1.00 11.00 GGGACACCACGAACAGAAGT
   RIGHT PRIMER      1929   20   59.97   50.00  5.00  2.00 12.00 GGCCAACACCTTGTCTTGAT
   PRODUCT SIZE: 225, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00