PRIMER PICKING RESULTS FOR NM_006182,Homo sapiens discoidin domain receptor family, member 2 (DDR2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2252   20   59.99   50.00  3.00  0.00 12.00 GGACCCAAACATCATCCATC
RIGHT PRIMER      2819   20   59.97   50.00  4.00  0.00 12.00 GTCAGGACAAATGGCTGGTT
SEQUENCE SIZE: 3096
INCLUDED REGION SIZE: 3096

PRODUCT SIZE: 568, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1910   20   60.01   55.00  6.00  3.00 10.00 CCACTATGCAGAGGCTGACA
   RIGHT PRIMER      2819   20   59.97   50.00  4.00  0.00 12.00 GTCAGGACAAATGGCTGGTT
   PRODUCT SIZE: 910, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       1910   20   60.01   55.00  6.00  3.00 10.00 CCACTATGCAGAGGCTGACA
   RIGHT PRIMER      2179   20   59.97   50.00  3.00  1.00 11.00 ATTTTCACAGCCACCAGGAC
   PRODUCT SIZE: 270, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1910   20   60.01   55.00  6.00  3.00 10.00 CCACTATGCAGAGGCTGACA
   RIGHT PRIMER      2092   20   60.04   50.00  4.00  0.00 11.00 CAGAGATGAACCTCCCCAAA
   PRODUCT SIZE: 183, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER        216   20   59.97   45.00  4.00  2.00 12.00 CCAAGGTTTGTGGCTTGAAT
   RIGHT PRIMER       509   20   60.02   55.00  5.00  1.00 12.00 CCACTGACTGGAAGCTGTGA
   PRODUCT SIZE: 294, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00