PRIMER PICKING RESULTS FOR NM_006206,Homo sapiens platelet-derived growth factor receptor, alpha polypeptide (PDGFRA), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1367   20   59.99   50.00  4.00  3.00 11.00 GAAGCTGTCAACCTGCATGA
RIGHT PRIMER      1553   20   59.98   55.00  4.00  2.00 11.00 CTTCCTTAGCACGGATCAGC
SEQUENCE SIZE: 6633
INCLUDED REGION SIZE: 6633

PRODUCT SIZE: 187, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       1367   20   59.99   50.00  4.00  3.00 11.00 GAAGCTGTCAACCTGCATGA
   RIGHT PRIMER      1551   20   59.98   50.00  4.00  2.00 11.00 TCCTTAGCACGGATCAGCTT
   PRODUCT SIZE: 185, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER       2396   20   60.02   50.00  4.00  0.00 11.00 GGCCCCATTTACATCATCAC
   RIGHT PRIMER      2555   20   60.01   50.00  4.00  1.00  9.00 CATAGCTCCGTGTGCTTTCA
   PRODUCT SIZE: 160, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER        361   20   60.03   50.00  3.00  1.00 11.00 CGGAATAACATCGGAGGAGA
   RIGHT PRIMER      1166   20   59.99   45.00  2.00  0.00 12.00 TGCCTTTGCCTTTCACTTCT
   PRODUCT SIZE: 806, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER        545   20   59.99   60.00  4.00  2.00 11.00 GGGGAGAGTGAAGTGAGCTG
   RIGHT PRIMER      1090   20   59.97   50.00  4.00  1.00 10.00 CACAATCGTTTCCCCTGACT
   PRODUCT SIZE: 546, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00