PRIMER PICKING RESULTS FOR NM_006293,Homo sapiens TYRO3 protein tyrosine kinase (TYRO3), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        556   20   59.99   55.00  3.00  2.00 11.00 CCTGGCAACCTCACACCTAT
RIGHT PRIMER      1050   20   59.99   50.00  3.00  2.00 11.00 AAATGGCACACCTTCTACCG
SEQUENCE SIZE: 4364
INCLUDED REGION SIZE: 4364

PRODUCT SIZE: 495, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       2641   20   59.99   60.00  4.00  1.00  9.00 GTGGCTGACTTCGGACTCTC
   RIGHT PRIMER      3435   20   60.01   55.00  4.00  2.00 10.00 AGCACAGCTTGGGAGGACTA
   PRODUCT SIZE: 795, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 2 LEFT PRIMER       1031   20   59.99   50.00  3.00  0.00 12.00 CGGTAGAAGGTGTGCCATTT
   RIGHT PRIMER      1754   20   59.98   55.00  5.00  2.00 11.00 TCCACTGTCAGCTCATCCTG
   PRODUCT SIZE: 724, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       2957   20   60.02   55.00  4.00  1.00 11.00 GCCCGAGCTTTACTTGTCTG
   RIGHT PRIMER      3435   20   60.01   55.00  4.00  2.00 10.00 AGCACAGCTTGGGAGGACTA
   PRODUCT SIZE: 479, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       2958   20   60.02   55.00  4.00  2.00 11.00 CCCGAGCTTTACTTGTCTGC
   RIGHT PRIMER      3435   20   60.01   55.00  4.00  2.00 10.00 AGCACAGCTTGGGAGGACTA
   PRODUCT SIZE: 478, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00