PRIMER PICKING RESULTS FOR NM_006343,Homo sapiens c-mer proto-oncogene tyrosine kinase (MERTK), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       1833   20   59.99   55.00  5.00  0.00 12.00 AGCTTGGGAGTCAGTGAGGA
RIGHT PRIMER      2262   20   60.02   50.00  4.00  0.00 11.00 GGGCAATATCCACCATGAAC
SEQUENCE SIZE: 3608
INCLUDED REGION SIZE: 3608

PRODUCT SIZE: 430, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

1 LEFT PRIMER       2065   20   59.97   50.00  2.00  1.00 12.00 ACTTCAGCCACCCAAATGTC
   RIGHT PRIMER      2402   20   59.99   50.00  6.00  3.00 10.00 GCGGTAATAATCGCCACTGT
   PRODUCT SIZE: 338, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       2064   20   59.97   50.00  2.00  2.00 12.00 GACTTCAGCCACCCAAATGT
   RIGHT PRIMER      2402   20   59.99   50.00  6.00  3.00 10.00 GCGGTAATAATCGCCACTGT
   PRODUCT SIZE: 339, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1833   20   59.99   55.00  5.00  0.00 12.00 AGCTTGGGAGTCAGTGAGGA
   RIGHT PRIMER      2084   20   59.97   50.00  2.00  1.00 12.00 GACATTTGGGTGGCTGAAGT
   PRODUCT SIZE: 252, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER       1833   20   59.99   55.00  5.00  0.00 12.00 AGCTTGGGAGTCAGTGAGGA
   RIGHT PRIMER      2083   20   59.97   50.00  2.00  2.00 12.00 ACATTTGGGTGGCTGAAGTC
   PRODUCT SIZE: 251, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00