PRIMER PICKING RESULTS FOR NM_007314,Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2), transcript variant b, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2363   20   60.00   55.00  6.00  1.00 10.00 GGAGCTTTGCACAGAGGAAC
RIGHT PRIMER      2671   20   59.99   60.00  3.00  2.00 10.00 CTGGCTGAGAAGAGGTGGAC
SEQUENCE SIZE: 3849
INCLUDED REGION SIZE: 3849

PRODUCT SIZE: 309, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

1 LEFT PRIMER       2364   20   60.00   55.00  6.00  2.00 10.00 GAGCTTTGCACAGAGGAACC
   RIGHT PRIMER      2671   20   59.99   60.00  3.00  2.00 10.00 CTGGCTGAGAAGAGGTGGAC
   PRODUCT SIZE: 308, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       2363   20   60.00   55.00  6.00  1.00 10.00 GGAGCTTTGCACAGAGGAAC
   RIGHT PRIMER      2517   20   60.03   55.00  4.00  1.00 12.00 ACTGGCTGTGGGTTTACCTG
   PRODUCT SIZE: 155, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00

 3 LEFT PRIMER       2363   20   60.00   55.00  6.00  1.00 10.00 GGAGCTTTGCACAGAGGAAC
   RIGHT PRIMER      2518   20   60.03   55.00  5.00  2.00 11.00 CACTGGCTGTGGGTTTACCT
   PRODUCT SIZE: 156, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER       2364   20   60.00   55.00  6.00  2.00 10.00 GAGCTTTGCACAGAGGAACC
   RIGHT PRIMER      2518   20   60.03   55.00  5.00  2.00 11.00 CACTGGCTGTGGGTTTACCT
   PRODUCT SIZE: 155, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00