PRIMER PICKING RESULTS FOR NM_013993,Homo sapiens discoidin domain receptor family, member 1 (DDR1), transcript variant 1, mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       3277   20   60.04   50.00  1.00  0.00 12.00 TCCCCTTCCTGTTCTTCCTT
RIGHT PRIMER      3631   20   60.00   55.00  3.00  0.00 11.00 CCAGGTCCAGTGTTTCAGGT
SEQUENCE SIZE: 3952
INCLUDED REGION SIZE: 3952

PRODUCT SIZE: 355, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER        518   20   60.06   55.00  3.00  1.00 12.00 ACAGCAGGTTGGAGAGCAGT
   RIGHT PRIMER       986   20   59.99   55.00  3.00  0.00  9.00 CCGTCATAGGTGGAGTCGTT
   PRODUCT SIZE: 469, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00

 2 LEFT PRIMER       2255   20   59.95   50.00  4.00  2.00 10.00 TCCCCCTTAATGTGCGTAAG
   RIGHT PRIMER      2739   20   60.02   55.00  4.00  2.00 10.00 GCCCTGCACACGGTAATAGT
   PRODUCT SIZE: 485, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00

 3 LEFT PRIMER        766   20   59.93   55.00  7.00  1.00 11.00 AATGAGGACCCTGAGGGAGT
   RIGHT PRIMER       986   20   59.99   55.00  3.00  0.00  9.00 CCGTCATAGGTGGAGTCGTT
   PRODUCT SIZE: 221, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

 4 LEFT PRIMER        410   20   60.08   45.00  4.00  2.00 10.00 ATTTTGATCCTGCCAAGTGC
   RIGHT PRIMER       986   20   59.99   55.00  3.00  0.00  9.00 CCGTCATAGGTGGAGTCGTT
   PRODUCT SIZE: 577, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00