PRIMER PICKING RESULTS FOR NM_014916,Homo sapiens kinase phosphatase inhibitor 2 (KPI2), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER       2921   20   59.99   55.00  5.00  2.00 11.00 CCACAGCCTGGATAACAGGT
RIGHT PRIMER      3917   20   59.99   50.00  6.00  0.00 10.00 TTCTGCATTCAGCACACTCC
SEQUENCE SIZE: 4954
INCLUDED REGION SIZE: 4954

PRODUCT SIZE: 997, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00

1 LEFT PRIMER       2700   20   59.99   50.00  5.00  2.00 10.00 TTGAGCACCTCATTGCAGTC
   RIGHT PRIMER      2940   20   59.99   55.00  5.00  2.00 12.00 ACCTGTTATCCAGGCTGTGG
   PRODUCT SIZE: 241, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 2 LEFT PRIMER       3195   20   59.98   60.00  4.00  1.00 12.00 CTCTCCTCGGACTCAACCAG
   RIGHT PRIMER      3917   20   59.99   50.00  6.00  0.00 10.00 TTCTGCATTCAGCACACTCC
   PRODUCT SIZE: 723, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       2700   20   59.99   50.00  5.00  2.00 10.00 TTGAGCACCTCATTGCAGTC
   RIGHT PRIMER      3214   20   59.98   60.00  4.00  0.00 12.00 CTGGTTGAGTCCGAGGAGAG
   PRODUCT SIZE: 515, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00

 4 LEFT PRIMER       3533   20   60.02   55.00  3.00  0.00 11.00 TGGTCACAGAGGCACAGAAG
   RIGHT PRIMER      3917   20   59.99   50.00  6.00  0.00 10.00 TTCTGCATTCAGCACACTCC
   PRODUCT SIZE: 385, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00