PRIMER PICKING RESULTS FOR NM_018423,Homo sapiens hypothetical protein DKFZp761P1010 (DKFZp761P1010), mRNA.

Using mispriming library humrep_and_simple.txt
Using 1-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq 
LEFT PRIMER        162   20   59.98   55.00  4.00  2.00 10.00 GGCTCAGGAAATGCAGTAGC
RIGHT PRIMER       524   20   59.93   45.00  3.00  2.00 11.00 CAAAGCCACATTTCCTCCAT
SEQUENCE SIZE: 2844
INCLUDED REGION SIZE: 2844

PRODUCT SIZE: 363, PAIR ANY COMPL: 8.00, PAIR 3' COMPL: 2.00

1 LEFT PRIMER        162   20   59.98   55.00  4.00  2.00 10.00 GGCTCAGGAAATGCAGTAGC
   RIGHT PRIMER      1039   20   59.92   50.00  7.00  1.00 12.00 ATATTCCTGGCTGCCACATC
   PRODUCT SIZE: 878, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00

 2 LEFT PRIMER        162   20   59.98   55.00  4.00  2.00 10.00 GGCTCAGGAAATGCAGTAGC
   RIGHT PRIMER       974   20   60.08   50.00  3.00  2.00 10.00 GACCTGCTTTCCGATGTGAT
   PRODUCT SIZE: 813, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00

 3 LEFT PRIMER       1239   20   60.00   55.00  3.00  2.00 11.00 GCACCACCGTATCCTGAAGT
   RIGHT PRIMER      1514   20   60.10   60.00  5.00  2.00 12.00 GAGGCTCTCCACTCTGATGC
   PRODUCT SIZE: 276, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00

 4 LEFT PRIMER        498   20   59.93   50.00  4.00  2.00 12.00 GCAGGACATGGAGGAAATGT
   RIGHT PRIMER      1214   20   60.04   50.00  6.00  0.00 10.00 CAGGATCCCAAAAGACCAGA
   PRODUCT SIZE: 717, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 1.00