PRIMER_SEQUENCE_ID=NM_002577
SEQUENCE=CCCCTTCCCTCCCCTCCCCTCCCCGCACCGCGCGCTAGCCCGGGGCGGCTCCGCAGCCCGCCGGGAGCTCTGACCGAGGCGCCTCGCTGGGGCGGGGACCTTGCCTTGCCCGGGGCCATTTCATAATTCTGAATCATGTCTGATAACGGAGAACTGGAAGATAAGCCTCCAGCACCTCCTGTGCGAATGAGCAGCACCATCTTTAGCACTGGAGGCAAAGACCCTTTGTCAGCCAATCACAGTTTGAAACCTTTGCCCTCTGTTCCAGAAGAGAAAAAGCCCAGGCATAAAATCATCTCCATATTCTCAGGCACAGAGAAAGGAAGTAAAAAGAAAGAAAAGGAACGGCCAGAAATTTCTCCTCCATCTGATTTTGAGCACACCATCCATGTTGGCTTTGATGCTGTTACTGGAGAATTCACTGGCATGCCAGAACAGTGGGCTCGATTACTACAGACCTCCAATATCACCAAACTAGAGCAAAAGAAGAATCCTCAGGCTGTGCTGGATGTCCTAAAGTTCTACGACTCCAACACAGTGAAGCAGAAATATCTGAGCTTTACTCCTCCTGAGAAAGATGGCTTTCCTTCTGGAACACCAGCACTGAATGCCAAGGGAACAGAAGCACCCGCAGTAGTGACAGAGGAGGAGGATGATGATGAAGAGACTGCTCCTCCCGTTATTGCCCCGCGACCGGATCATACGAAATCAATTTACACACGGTCTGTAATTGACCCTGTTCCTGCACCAGTTGGTGATTCACATGTTGATGGTGCTGCCAAGTCTTTAGACAAACAGAAAAAGAAGACTAAGATGACAGATGAAGAGATTATGGAGAAATTAAGAACTATCGTGAGCATAGGTGACCCTAAGAAAAAATATACAAGATATGAAAAAATTGGACAAGGGGCTTCTGGTACAGTTTTCACTGCTACTGACGTTGCACTGGGACAGGAGGTTGCTATCAAACAAATTAATTTACAGAAACAGCCAAAGAAGGAACTGATCATTAACGAGATTCTGGTGATGAAAGAATTGAAAAATCCCAACATCGTTAACTTTTTGGACAGTTACCTGGTAGGAGATGAATTGTTTGTGGTCATGGAATACCTTGCTGGGGGGTCACTCACTGATGTGGTAACAGAAACGTGCATGGATGAAGCACAGATTGCTGCTGTATGCAGAGAGTGTTTACAGGCATTGGAGTTTTTACATGCTAATCAAGTGATCCACAGAGACATCAAAAGTGACAATGTACTTTTGGGAATGGAAGGATCTGTTAAGCTCACTGACTTTGGTTTCTGTGCCCAGATCACCCCTGAGCAGAGCAAACGCAGTACCATGGTCGGAACGCCATACTGGATGGCACCAGAGGTGGTTACACGGAAAGCTTATGGCCCTAAAGTCGACATATGGTCTCTGGGTATCATGGCTATTGAGATGGTAGAAGGAGAGCCTCCATACCTCAATGAAAATCCCTTGAGGGCCTTGTACCTAATAGCAACTAATGGAACCCCAGAACTTCAGAATCCAGAGAAACTTTCCCCAATATTTCGGGATTTCTTAAATCGATGTTTGGAAATGGATGTGGAAAAAAGGGGTTCAGCCAAAGAATTATTACAGCATCCTTTCCTGAAACTGGCCAAACCGTTATCTAGCTTGACACCACTGATCATGGCAGCTAAAGAAGCAATGAAGAGTAACCGTTAACATCACTGCTGTGGCCTCATACTCTTTTTTCCATTTTCTACAAGAAGCCTTTTAGTATATGAAAATTATTACTCTTTTTGGGGTTTAAAGAAATGGTCTGCATAACCTGAATGAAAGAAGCAAATGACTATTCTCTGAAGACAACCAAGAGAAAATTGCAAAAAGACAAGTATGACTTTTATATGAACCCCTTCTTTAGGGTCCAGAAGGAATTGTGGACTGAATCACTAGCCTTAGGTCTTTCAGCAAACAGCCTATCAGGGCCATTTATCATGTGTGAGATTTGCATTTTACTTTGCTGACTTTGTTGTAATAGATCCCATTCATTGTCCCCTTTGGGGTATTTCCAATACTTGAATGGCAGATTGGAGTTTTTCAGAGTATGTGTTTCATCTGCTAGTCTTTCTCTCCTTCATAGCTTTTCTTTTCCTGGACTTGCTCCTTTTGAGTTGCTTTTGCGTTTCTCATGCCTAGGCAAGTGTAATAGAAATTATGTAGCTCCTTATGTTGGCAAAGGAGCTCTATATAGTTTCACTTTGTATAAAAGTTAGGACCAGCTGTTGTTACATGTAATATTTTAGTTCAGAACTTGACCTGAAGGAAGGGAAGAAAAGTATGTGATTTTTACCTTTTTTAACAAATGTGAAAAAGTCAGTTTTAGAAATTTCGTGGTAGTAAGTTCGGCATTTGTTACATGTATAGAGAGAAGACTAATAATCTCTATTTATAACTAAATCATTGAGATAGAAAAAGATTCCCATTGACTGTAGACTTCTTCCCATTTTGTCTTCCCTTCTGCCTGTTTCCCCTTCAGGCTTGGCTCTAGGAACCAAAGTGATTTGTTGTTGTTCCAACCTGGGCTTTGTGACTTTGGTTAGTGCCACTACCTTCTTCCCTCCTTTCCCCCTTCAATTTGGAAATAAATTTCTGTATATGTTGCAATTTTAGGTTTAGGTTTGTTCTTTTTCTTTTTCATTAATCCTCTCTCACCTCACAGATACCCCCTCCCATGGCAAATAATATAATAACCAGTGAATTTTCAGGAATTTAAAAATTAGCTTTTTTCCACTTAAAGGAGAAAAATATTTGGGACTAGCAGCAGAGGCAGTAAGAGATGTGAACCTTGGTGAGCTCTGATACAGTGAGAAGAGATTATACTCATGAAAGAGAATGTTAGTGTTACAGAGAAGCAGCCGATAGCAAATCGACTGTAGAGACTTGGCGGCGGTGGCATTGCCCCAGGTCGTCAGCAGTGTGGTATTATCTATGAGAACTTGAGCGACAGAGTATTTCTTGATGAATTTATAGATCATTTGAGATGTTGAGTTACTTTAGTTTAGTTTTGTTTTGTTTTTTCAAATAAGTAGAGACTATTGTAAAAAACGAGAAAGGAAAATGAAATGTGCGTGTTGATAGCAATAATTTGTTTCTTTTAAAGATTCTAAAAGGTCTGAGACCTGTAGCATTAATTATTTGAGTGCCCTCCCTTCTCCCCTCCCCTCCCTTTTCTCTTCTCTTTTTTCCTCTCCTCTCCTTCTCCTTTATTCATTGTTTTGCTTTTGGAGTAGGTGTTGTTCAAGTATCTGTGGTTTGGTTCTGGCATTTTGTTCCCACCATCCCCTTCCCCCATTAACTTCCCCCCTGCTTGCCATCCTGCAGTAGTATAAATCATGAATAAAAAATAATTTTGCTGTTGTAGTATACATTGGAGAAACTGGCAGGTTTTATTTCCATTATTTTATTTCCACTATATCTATGATAAGATGCAATTATAAGGAGAGAAGTGACTGTTTTTTATTGATAAGGCAAGATTTTCAGAAAAATGAGTAAAATAATTAATGAAACATATTTAGAGCACTTAATGGTCTCTGTTTTCAATATAATTCTTGATTTCATTTTTCTCTGGAATATATTGGCCTTCTACAGCTATTACTGAATTATAGAAACTGGTTTATTTCTGGCAGAAAGCTGCAGTGCCACCTGAGTTCCAAATTTTACCATTCTTTGTAAACAGTTGGATGGATTATGATAAAGAAGATGCTACCAATGAAATAGAAAACCAACGAGATGAGAAGACTGTGATCCTCATGTACTCAGAGGCACTTCCCTCCTAAGTCAAAGACCATCCTCACTGACTATGTGCCAACGCCTCGTTTCAGGCTTGTGACTCAACAAAGGGCTTTTCCATTGATAGAAGCAGTTTGGGATTTGTAGTTGCGACTTCTTCGATAGTTACCTGCACGTCCATTGCTGGCAACTGACTTGTCATTAAAACCTGGCTCTTTGGTTAAGGGAGCTACGCTGTGGTTTATTCTTAAGTTACGTGGATAAACTAACCTCTAACAGAAATATACTTTGGTTAATTTTGAAATGTGTCATTTTTAAACAATCTTAAAAGTAATACAGAATTGTGATTTATTAATTTTAAAACATTCAGAACTTGTTGAAAGAAAAATTATATCTGAATCAAGATTCATGTTTTTTATTTTTATTTTTTTTGATACAGAGTCTCACTCTGTCACTCAGGCTGGAGTGCAGTGACATGATCTCAGCTCACTGCAACCTCCGCTTCCTGGGTTCAAGCAATTCTCATGCCTCAGCCTCCTGAGTAGCTGAGACCACAGGCACCCGCCACCACACCCAGCTATTTTTTTGTATTTTTAGTAGAGACAGAGTCTCACCACATTGCCCAGGCTGGTCTCCAACTCCTGAGCTCAGGCAGTCTGCCCACCTTGGCCTCCCAAAGTGCTGCAGTTACAGGCGTGAGCCACTGCACCTGGCCTCATGTTTTTTAAATAATTGCCTTTTATATTTACCCTTTTTGTCATCACTTTAGAATGAAAATTCCCATTTAAATCTGAAAGTTACCTTAATAGTCCTCTTGTGTTATTAGGACAGTATTATTATAGTACTTATTTATTTTATTTTAGATTTAAAGTTATCTTCTCTTTTTCTTTTTTCTTCTGCTGCTTTTAGGGACAATTAAAACTGGGAAACTATGAAACATGGAACATTTTATCCTACCTGAAAGTAAACGAGTAATTGTGAAGCATAAGACACTGAGGCTAATACAACTCTGTCTTCATGTGTTGACTGCCTGGCACATAGTATTCATTCTCTTCCCTTTAACATAGAAGTGTCCAGCTGCGTACAGTCTAGTAACCAGCAACTGTAAACGAACCTGTGCCTCTAACAAGCGATTCTAAACCACCTATGAGTATTTCTTTTAGGGCTCACTTAAATACATGTTTGTATATACTGTATTCTAGCCAGAATAATTTTAGATCTGATCAGGTAGTAGCTAAAATTAGAAAAAAACAAAATAGATGCTTAAAGAATTTGCATCCATTTTTGAGTCTAAATCTTTTAAAATATACTGAGATCCACATCTAGTGAAATGTCAGTGTCAAAATATTATAGATTATAGCTAAAATCCAGATTAATACTCATTTGGGGTTTTTTATAGTGGAACTTCATAGTAATACAAAAAGCAGATTGTCTTCCTGTCTCCGCTGCTCCCACAGTAGGTATTGAAACTGGTAAAATCAGTTTTTTGATAGTGTGTGTATATAAGAAAAAATAGATACACACATTCTTTTTTCTCAGTCAACACATTGATTGAACACTCTGGCAAAGATGCTGTGGTGGATGAGGTTGGAGTTCGAAAGAAGAAGCAAGCGCTGGCCTGGCCTTGAAAGAACCGAAGTCTTTCCCATTCACTTCTCTAGAAAGCTGCCAAGACAGAGGCAGAAAGAAATGGATGATAGTTCTGTCAAGCACACTTCTGTTCTCTTAGAACTTAGAAGTGTTTCTAAGAGAACAGAAGTAATAAGAGAAACAGTTACGTGTGGAATTCAACATCTTTGGTTGGAACGCATTGGCTTTTTTTTTCTTGTTTTGATAGAAATGGAATTAAGCAAAAGTAGTTTTTGTCTTTTCTGTTGTCTTCAAATTTTATGCCTTTTATTTTTAATTTAATCCCGTTCAATTATTTAATTGTTATACATTGACATTAACTGCTGTATTTTGACTTTGTTCAATAATTTTGTTCTTTCAGGGCTAGAAATAAACTTTTTAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 65300, GC content failed 5253, low tm 36132, high tm 10855, high any compl 6, high end compl 26, high repeat similarity 56, long poly-x seq 441, ok 12531
PRIMER_RIGHT_EXPLAIN=considered 65302, GC content failed 4891, low tm 36718, high tm 10557, high end compl 12, high repeat similarity 11, long poly-x seq 447, ok 12666
PRIMER_PAIR_EXPLAIN=considered 1777, unacceptable product size 1719, high end compl 4, ok 54
PRIMER_PAIR_PENALTY=0.0205
PRIMER_LEFT_PENALTY=0.006308
PRIMER_RIGHT_PENALTY=0.014220
PRIMER_LEFT_SEQUENCE=CTCTGGGTATCATGGCTATTGAG
PRIMER_RIGHT_SEQUENCE=TCAGGTTATGCAGACCATTTCTT
PRIMER_LEFT=1417,23
PRIMER_RIGHT=1819,23
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=403
PRIMER_PAIR_PENALTY_1=0.0205
PRIMER_LEFT_1_PENALTY=0.006308
PRIMER_RIGHT_1_PENALTY=0.014220
PRIMER_LEFT_1_SEQUENCE=CTCTGGGTATCATGGCTATTGAG
PRIMER_RIGHT_1_SEQUENCE=TTCAGGTTATGCAGACCATTTCT
PRIMER_LEFT_1=1417,23
PRIMER_RIGHT_1=1820,23
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MER31 a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=404
PRIMER_PAIR_PENALTY_2=0.0427
PRIMER_LEFT_2_PENALTY=0.028502
PRIMER_RIGHT_2_PENALTY=0.014220
PRIMER_LEFT_2_SEQUENCE=AAGCTCACTGACTTTGGTTTCTG
PRIMER_RIGHT_2_SEQUENCE=TCAGGTTATGCAGACCATTTCTT
PRIMER_LEFT_2=1281,23
PRIMER_RIGHT_2=1819,23
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=539
PRIMER_PAIR_PENALTY_3=0.0427
PRIMER_LEFT_3_PENALTY=0.028502
PRIMER_RIGHT_3_PENALTY=0.014220
PRIMER_LEFT_3_SEQUENCE=AAGCTCACTGACTTTGGTTTCTG
PRIMER_RIGHT_3_SEQUENCE=TTCAGGTTATGCAGACCATTTCT
PRIMER_LEFT_3=1281,23
PRIMER_RIGHT_3=1820,23
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MER31 a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=540
PRIMER_PAIR_PENALTY_4=0.0446
PRIMER_LEFT_4_PENALTY=0.035742
PRIMER_RIGHT_4_PENALTY=0.008825
PRIMER_LEFT_4_SEQUENCE=TGCGTGTTGATAGCAATAATTTG
PRIMER_RIGHT_4_SEQUENCE=AGGATGGTCTTTGACTTAGGAGG
PRIMER_LEFT_4=3113,23
PRIMER_RIGHT_4=3838,23
PRIMER_LEFT_4_TM=60.036
PRIMER_RIGHT_4_TM=60.009
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=726
PRIMER_PAIR_PENALTY_5=0.0454
PRIMER_LEFT_5_PENALTY=0.008825
PRIMER_RIGHT_5_PENALTY=0.036623
PRIMER_LEFT_5_SEQUENCE=CCTCCTAAGTCAAAGACCATCCT
PRIMER_RIGHT_5_SEQUENCE=AGCCTCAGTGTCTTATGCTTCAC
PRIMER_LEFT_5=3816,23
PRIMER_RIGHT_5=4776,23
PRIMER_LEFT_5_TM=60.009
PRIMER_RIGHT_5_TM=59.963
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.8000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=961
PRIMER_PAIR_PENALTY_6=0.0518
PRIMER_LEFT_6_PENALTY=0.011619
PRIMER_RIGHT_6_PENALTY=0.040223
PRIMER_LEFT_6_SEQUENCE=ATGTCTGATAACGGAGAACTGGA
PRIMER_RIGHT_6_SEQUENCE=TCTCCTACCAGGTAACTGTCCAA
PRIMER_LEFT_6=135,23
PRIMER_RIGHT_6=1084,23
PRIMER_LEFT_6_TM=60.012
PRIMER_RIGHT_6_TM=60.040
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=7.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_6=950
PRIMER_PAIR_PENALTY_7=0.0522
PRIMER_LEFT_7_PENALTY=0.043423
PRIMER_RIGHT_7_PENALTY=0.008825
PRIMER_LEFT_7_SEQUENCE=CATTGTTTTGCTTTTGGAGTAGG
PRIMER_RIGHT_7_SEQUENCE=AGGATGGTCTTTGACTTAGGAGG
PRIMER_LEFT_7=3256,23
PRIMER_RIGHT_7=3838,23
PRIMER_LEFT_7_TM=60.043
PRIMER_RIGHT_7_TM=60.009
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, LTR4 LTR from human endogenous retrovirus ERV3, POL-ENV-3'LTR region
PRIMER_LEFT_7_END_STABILITY=6.9000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=583
PRIMER_PAIR_PENALTY_8=0.0544
PRIMER_LEFT_8_PENALTY=0.040223
PRIMER_RIGHT_8_PENALTY=0.014220
PRIMER_LEFT_8_SEQUENCE=TTGGACAGTTACCTGGTAGGAGA
PRIMER_RIGHT_8_SEQUENCE=TCAGGTTATGCAGACCATTTCTT
PRIMER_LEFT_8=1062,23
PRIMER_RIGHT_8=1819,23
PRIMER_LEFT_8_TM=60.040
PRIMER_RIGHT_8_TM=60.014
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=758
PRIMER_PAIR_PENALTY_9=0.0544
PRIMER_LEFT_9_PENALTY=0.040223
PRIMER_RIGHT_9_PENALTY=0.014220
PRIMER_LEFT_9_SEQUENCE=TTGGACAGTTACCTGGTAGGAGA
PRIMER_RIGHT_9_SEQUENCE=TTCAGGTTATGCAGACCATTTCT
PRIMER_LEFT_9=1062,23
PRIMER_RIGHT_9=1820,23
PRIMER_LEFT_9_TM=60.040
PRIMER_RIGHT_9_TM=60.014
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, MER31 a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.9000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=759
PRIMER_PAIR_PENALTY_10=0.0558
PRIMER_LEFT_10_PENALTY=0.040223
PRIMER_RIGHT_10_PENALTY=0.015613
PRIMER_LEFT_10_SEQUENCE=TTGGACAGTTACCTGGTAGGAGA
PRIMER_RIGHT_10_SEQUENCE=AGCTTAACAGATCCTTCCATTCC
PRIMER_LEFT_10=1062,23
PRIMER_RIGHT_10=1285,23
PRIMER_LEFT_10_TM=60.040
PRIMER_RIGHT_10_TM=59.984
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=7.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=8.1000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=224
PRIMER_PAIR_PENALTY_11=0.0591
PRIMER_LEFT_11_PENALTY=0.044904
PRIMER_RIGHT_11_PENALTY=0.014220
PRIMER_LEFT_11_SEQUENCE=TATGCAGAGAGTGTTTACAGGCA
PRIMER_RIGHT_11_SEQUENCE=TCAGGTTATGCAGACCATTTCTT
PRIMER_LEFT_11=1177,23
PRIMER_RIGHT_11=1819,23
PRIMER_LEFT_11_TM=59.955
PRIMER_RIGHT_11_TM=60.014
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=643
PRIMER_PAIR_PENALTY_12=0.0591
PRIMER_LEFT_12_PENALTY=0.044904
PRIMER_RIGHT_12_PENALTY=0.014220
PRIMER_LEFT_12_SEQUENCE=TATGCAGAGAGTGTTTACAGGCA
PRIMER_RIGHT_12_SEQUENCE=TTCAGGTTATGCAGACCATTTCT
PRIMER_LEFT_12=1177,23
PRIMER_RIGHT_12=1820,23
PRIMER_LEFT_12_TM=59.955
PRIMER_RIGHT_12_TM=60.014
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, MER31 a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=644
PRIMER_PAIR_PENALTY_13=0.0605
PRIMER_LEFT_13_PENALTY=0.044904
PRIMER_RIGHT_13_PENALTY=0.015613
PRIMER_LEFT_13_SEQUENCE=TATGCAGAGAGTGTTTACAGGCA
PRIMER_RIGHT_13_SEQUENCE=AGCTTAACAGATCCTTCCATTCC
PRIMER_LEFT_13=1177,23
PRIMER_RIGHT_13=1285,23
PRIMER_LEFT_13_TM=59.955
PRIMER_RIGHT_13_TM=59.984
PRIMER_LEFT_13_GC_PERCENT=43.478
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=109
PRIMER_PAIR_PENALTY_14=0.0626
PRIMER_LEFT_14_PENALTY=0.048411
PRIMER_RIGHT_14_PENALTY=0.014220
PRIMER_LEFT_14_SEQUENCE=ACAGGAGGTTGCTATCAAACAAA
PRIMER_RIGHT_14_SEQUENCE=TTCAGGTTATGCAGACCATTTCT
PRIMER_LEFT_14=950,23
PRIMER_RIGHT_14=1820,23
PRIMER_LEFT_14_TM=60.048
PRIMER_RIGHT_14_TM=60.014
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MER31 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.0000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=871
PRIMER_PAIR_PENALTY_15=0.0640
PRIMER_LEFT_15_PENALTY=0.048411
PRIMER_RIGHT_15_PENALTY=0.015613
PRIMER_LEFT_15_SEQUENCE=ACAGGAGGTTGCTATCAAACAAA
PRIMER_RIGHT_15_SEQUENCE=AGCTTAACAGATCCTTCCATTCC
PRIMER_LEFT_15=950,23
PRIMER_RIGHT_15=1285,23
PRIMER_LEFT_15_TM=60.048
PRIMER_RIGHT_15_TM=59.984
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=1.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.0000
PRIMER_RIGHT_15_END_STABILITY=8.1000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=336
PRIMER_PAIR_PENALTY_16=0.0641
PRIMER_LEFT_16_PENALTY=0.009608
PRIMER_RIGHT_16_PENALTY=0.054491
PRIMER_LEFT_16_SEQUENCE=CAGAAGGAATTGTGGACTGAATC
PRIMER_RIGHT_16_SEQUENCE=AACAAATGCCGAACTTACTACCA
PRIMER_LEFT_16=1913,23
PRIMER_RIGHT_16=2401,23
PRIMER_LEFT_16_TM=59.990
PRIMER_RIGHT_16_TM=59.946
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=3.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER35 a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.6000
PRIMER_RIGHT_16_END_STABILITY=7.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=489
PRIMER_PAIR_PENALTY_17=0.0687
PRIMER_LEFT_17_PENALTY=0.014220
PRIMER_RIGHT_17_PENALTY=0.054491
PRIMER_LEFT_17_SEQUENCE=AAGAAATGGTCTGCATAACCTGA
PRIMER_RIGHT_17_SEQUENCE=AACAAATGCCGAACTTACTACCA
PRIMER_LEFT_17=1797,23
PRIMER_RIGHT_17=2401,23
PRIMER_LEFT_17_TM=60.014
PRIMER_RIGHT_17_TM=59.946
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=21.00, MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_17_END_STABILITY=8.2000
PRIMER_RIGHT_17_END_STABILITY=7.2000
PRIMER_PAIR_17_COMPL_ANY=6.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=605
PRIMER_PAIR_PENALTY_18=0.0715
PRIMER_LEFT_18_PENALTY=0.055869
PRIMER_RIGHT_18_PENALTY=0.015613
PRIMER_LEFT_18_SEQUENCE=AATCATCTCCATATTCTCAGGCA
PRIMER_RIGHT_18_SEQUENCE=AGCTTAACAGATCCTTCCATTCC
PRIMER_LEFT_18=290,23
PRIMER_RIGHT_18=1285,23
PRIMER_LEFT_18_TM=59.944
PRIMER_RIGHT_18_TM=59.984
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=20.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=996
PRIMER_PAIR_PENALTY_19=0.0759
PRIMER_LEFT_19_PENALTY=0.061667
PRIMER_RIGHT_19_PENALTY=0.014220
PRIMER_LEFT_19_SEQUENCE=GGGCCTTGTACCTAATAGCAACT
PRIMER_RIGHT_19_SEQUENCE=TCAGGTTATGCAGACCATTTCTT
PRIMER_LEFT_19=1483,23
PRIMER_RIGHT_19=1819,23
PRIMER_LEFT_19_TM=59.938
PRIMER_RIGHT_19_TM=60.014
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=337
=