PRIMER_SEQUENCE_ID=NM_005884
SEQUENCE=CCAGTGTGGTGGAATTCGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGGGAGGCGCGAGGGCGCGGAGTTCCAGGTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGATGAGTAACCCGAAGCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCCACCAGCATCAGACCAGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTTCGCCGGTCACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGGCGGGGCCAGAGAAGAGGCCCAAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAAACGCCCCCTCTCCGGGCCTGATGTCGGCACCCCCCAGCCTGCTGGTCTGGCCAGTGGGGCGAAACTGGCAGCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACGGACCACCCATCCCGGGGTGCCCAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGCCATCCCCCAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGAGGTGCCCCCAGCCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGCCTGCACCCCCGCCGCCCCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTACCTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACCTGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGTGGTGGAGATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGCGGCCGTGTGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGGTGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCCCTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGGTGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGAGGCCCAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCCAGTAGGGGGCCAGGCCTCCCACTCCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCCTTGCTGGGGGTAGATGAGACCCTACTACTGAACTCCAGTTTTGATCTCGTGACTTTTAGAAAAACACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGGCCCTCCCCCATGTTCTTCTGTCTCCAGGAAGGGCAGCGGCCCTCCCATCACTGGAAGTCTGCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTGTGCACGCGTGTGAGTGTGCATGTGTGTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCCCGCAAGGGGTGTCTGGCGCCTTGCCTGACACCCAGCCCCCTCTCCCCCTGAGCCATTGTGGGGGTCGATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTGTGGCTGGATGGGGAGACAGGTCAGGGCCCCCCACCCTCTCCAGCCCCTGCAGCAAATGACTACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATAACACTCTAGCCCCTGCCCTTATTGGGGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCAGAACCACTGCCTGAAGAACCAGGTTCCTGCCCGGTCAGCGCAGCCCCAGCCCGCCCACCCCTGCCTCGAGTTAGTTTTACAATTAAAACATTGTCTTGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 32144, GC content failed 2022, low tm 4905, high tm 20441, high end compl 64, high repeat similarity 66, long poly-x seq 99, ok 4547
PRIMER_RIGHT_EXPLAIN=considered 32094, GC content failed 2460, low tm 5013, high tm 19911, high end compl 20, high repeat similarity 13, long poly-x seq 105, ok 4572
PRIMER_PAIR_EXPLAIN=considered 1372, unacceptable product size 1299, high end compl 12, ok 61
PRIMER_PAIR_PENALTY=0.5552
PRIMER_LEFT_PENALTY=0.197354
PRIMER_RIGHT_PENALTY=0.357879
PRIMER_LEFT_SEQUENCE=ACTGAACTCCAGTTTTGATCTCG
PRIMER_RIGHT_SEQUENCE=GCACACACTCATACATGTTCACC
PRIMER_LEFT=2136,23
PRIMER_RIGHT=2349,23
PRIMER_LEFT_TM=59.803
PRIMER_RIGHT_TM=60.358
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.5632
PRIMER_LEFT_1_PENALTY=0.197354
PRIMER_RIGHT_1_PENALTY=0.365880
PRIMER_LEFT_1_SEQUENCE=ACTGAACTCCAGTTTTGATCTCG
PRIMER_RIGHT_1_SEQUENCE=TTCTAGAAAAGAGGAGGCTGTCC
PRIMER_LEFT_1=2136,23
PRIMER_RIGHT_1=2622,23
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=60.366
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=487
PRIMER_PAIR_PENALTY_2=0.6756
PRIMER_LEFT_2_PENALTY=0.317733
PRIMER_RIGHT_2_PENALTY=0.357879
PRIMER_LEFT_2_SEQUENCE=CCAGTTTTGATCTCGTGACTTTT
PRIMER_RIGHT_2_SEQUENCE=GCACACACTCATACATGTTCACC
PRIMER_LEFT_2=2144,23
PRIMER_RIGHT_2=2349,23
PRIMER_LEFT_2_TM=59.682
PRIMER_RIGHT_2_TM=60.358
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.6836
PRIMER_LEFT_3_PENALTY=0.317733
PRIMER_RIGHT_3_PENALTY=0.365880
PRIMER_LEFT_3_SEQUENCE=CCAGTTTTGATCTCGTGACTTTT
PRIMER_RIGHT_3_SEQUENCE=TTCTAGAAAAGAGGAGGCTGTCC
PRIMER_LEFT_3=2144,23
PRIMER_RIGHT_3=2622,23
PRIMER_LEFT_3_TM=59.682
PRIMER_RIGHT_3_TM=60.366
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=479
PRIMER_PAIR_PENALTY_4=0.7238
PRIMER_LEFT_4_PENALTY=0.357879
PRIMER_RIGHT_4_PENALTY=0.365880
PRIMER_LEFT_4_SEQUENCE=GGTGAACATGTATGAGTGTGTGC
PRIMER_RIGHT_4_SEQUENCE=TTCTAGAAAAGAGGAGGCTGTCC
PRIMER_LEFT_4=2327,23
PRIMER_RIGHT_4=2622,23
PRIMER_LEFT_4_TM=60.358
PRIMER_RIGHT_4_TM=60.366
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
PRIMER_PAIR_PENALTY_5=0.7913
PRIMER_LEFT_5_PENALTY=0.197354
PRIMER_RIGHT_5_PENALTY=0.593947
PRIMER_LEFT_5_SEQUENCE=ACTGAACTCCAGTTTTGATCTCG
PRIMER_RIGHT_5_SEQUENCE=CTGTCCAGGTGCAGTAGTCATTT
PRIMER_LEFT_5=2136,23
PRIMER_RIGHT_5=2605,23
PRIMER_LEFT_5_TM=59.803
PRIMER_RIGHT_5_TM=60.594
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.4000
PRIMER_RIGHT_5_END_STABILITY=7.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=470
PRIMER_PAIR_PENALTY_6=0.9077
PRIMER_LEFT_6_PENALTY=0.549817
PRIMER_RIGHT_6_PENALTY=0.357879
PRIMER_LEFT_6_SEQUENCE=TTAGAAAAACACAGGGACTCGTG
PRIMER_RIGHT_6_SEQUENCE=GCACACACTCATACATGTTCACC
PRIMER_LEFT_6=2165,23
PRIMER_RIGHT_6=2349,23
PRIMER_LEFT_6_TM=60.550
PRIMER_RIGHT_6_TM=60.358
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=8.4000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=185
PRIMER_PAIR_PENALTY_7=0.9117
PRIMER_LEFT_7_PENALTY=0.317733
PRIMER_RIGHT_7_PENALTY=0.593947
PRIMER_LEFT_7_SEQUENCE=CCAGTTTTGATCTCGTGACTTTT
PRIMER_RIGHT_7_SEQUENCE=CTGTCCAGGTGCAGTAGTCATTT
PRIMER_LEFT_7=2144,23
PRIMER_RIGHT_7=2605,23
PRIMER_LEFT_7_TM=59.682
PRIMER_RIGHT_7_TM=60.594
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.3000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=462
PRIMER_PAIR_PENALTY_8=0.9157
PRIMER_LEFT_8_PENALTY=0.549817
PRIMER_RIGHT_8_PENALTY=0.365880
PRIMER_LEFT_8_SEQUENCE=TTAGAAAAACACAGGGACTCGTG
PRIMER_RIGHT_8_SEQUENCE=TTCTAGAAAAGAGGAGGCTGTCC
PRIMER_LEFT_8=2165,23
PRIMER_RIGHT_8=2622,23
PRIMER_LEFT_8_TM=60.550
PRIMER_RIGHT_8_TM=60.366
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.4000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=458
PRIMER_PAIR_PENALTY_9=0.9518
PRIMER_LEFT_9_PENALTY=0.357879
PRIMER_RIGHT_9_PENALTY=0.593947
PRIMER_LEFT_9_SEQUENCE=GGTGAACATGTATGAGTGTGTGC
PRIMER_RIGHT_9_SEQUENCE=CTGTCCAGGTGCAGTAGTCATTT
PRIMER_LEFT_9=2327,23
PRIMER_RIGHT_9=2605,23
PRIMER_LEFT_9_TM=60.358
PRIMER_RIGHT_9_TM=60.594
PRIMER_LEFT_9_GC_PERCENT=47.826
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.2000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=279
PRIMER_PAIR_PENALTY_10=1.1438
PRIMER_LEFT_10_PENALTY=0.549817
PRIMER_RIGHT_10_PENALTY=0.593947
PRIMER_LEFT_10_SEQUENCE=TTAGAAAAACACAGGGACTCGTG
PRIMER_RIGHT_10_SEQUENCE=CTGTCCAGGTGCAGTAGTCATTT
PRIMER_LEFT_10=2165,23
PRIMER_RIGHT_10=2605,23
PRIMER_LEFT_10_TM=60.550
PRIMER_RIGHT_10_TM=60.594
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_LEFT_10_END_STABILITY=8.4000
PRIMER_RIGHT_10_END_STABILITY=7.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=441
PRIMER_PAIR_PENALTY_11=1.2038
PRIMER_LEFT_11_PENALTY=0.197354
PRIMER_RIGHT_11_PENALTY=1.006403
PRIMER_LEFT_11_SEQUENCE=ACTGAACTCCAGTTTTGATCTCG
PRIMER_RIGHT_11_SEQUENCE=CCTTCCTGGAGACAGAAGAACAT
PRIMER_LEFT_11=2136,23
PRIMER_RIGHT_11=2261,23
PRIMER_LEFT_11_TM=59.803
PRIMER_RIGHT_11_TM=61.006
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_PAIR_11_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.4000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=126
PRIMER_PAIR_PENALTY_12=1.2095
PRIMER_LEFT_12_PENALTY=0.197354
PRIMER_RIGHT_12_PENALTY=1.012153
PRIMER_LEFT_12_SEQUENCE=ACTGAACTCCAGTTTTGATCTCG
PRIMER_RIGHT_12_SEQUENCE=CTAGAAAAGAGGAGGCTGTCCA
PRIMER_LEFT_12=2136,23
PRIMER_RIGHT_12=2620,22
PRIMER_LEFT_12_TM=59.803
PRIMER_RIGHT_12_TM=60.012
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_12_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=485
PRIMER_PAIR_PENALTY_13=1.2700
PRIMER_LEFT_13_PENALTY=0.966648
PRIMER_RIGHT_13_PENALTY=0.303373
PRIMER_LEFT_13_SEQUENCE=GAGTTTGAGAACATGTCGGTGAC
PRIMER_RIGHT_13_SEQUENCE=GTACATCTCCACCACATTCTCGT
PRIMER_LEFT_13=471,23
PRIMER_RIGHT_13=1355,23
PRIMER_LEFT_13_TM=60.967
PRIMER_RIGHT_13_TM=60.303
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=3.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.1000
PRIMER_RIGHT_13_END_STABILITY=8.1000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=885
PRIMER_PAIR_PENALTY_14=1.3241
PRIMER_LEFT_14_PENALTY=0.317733
PRIMER_RIGHT_14_PENALTY=1.006403
PRIMER_LEFT_14_SEQUENCE=CCAGTTTTGATCTCGTGACTTTT
PRIMER_RIGHT_14_SEQUENCE=CCTTCCTGGAGACAGAAGAACAT
PRIMER_LEFT_14=2144,23
PRIMER_RIGHT_14=2261,23
PRIMER_LEFT_14_TM=59.682
PRIMER_RIGHT_14_TM=61.006
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.3000
PRIMER_RIGHT_14_END_STABILITY=6.6000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=118
PRIMER_PAIR_PENALTY_15=1.3299
PRIMER_LEFT_15_PENALTY=0.317733
PRIMER_RIGHT_15_PENALTY=1.012153
PRIMER_LEFT_15_SEQUENCE=CCAGTTTTGATCTCGTGACTTTT
PRIMER_RIGHT_15_SEQUENCE=CTAGAAAAGAGGAGGCTGTCCA
PRIMER_LEFT_15=2144,23
PRIMER_RIGHT_15=2620,22
PRIMER_LEFT_15_TM=59.682
PRIMER_RIGHT_15_TM=60.012
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1b (MLT1b subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.3000
PRIMER_RIGHT_15_END_STABILITY=7.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=477
PRIMER_PAIR_PENALTY_16=1.3532
PRIMER_LEFT_16_PENALTY=0.966648
PRIMER_RIGHT_16_PENALTY=0.386599
PRIMER_LEFT_16_SEQUENCE=GAGTTTGAGAACATGTCGGTGAC
PRIMER_RIGHT_16_SEQUENCE=TGCTGGTAGTCCCTCATGATTAC
PRIMER_LEFT_16=471,23
PRIMER_RIGHT_16=1333,23
PRIMER_LEFT_16_TM=60.967
PRIMER_RIGHT_16_TM=60.387
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=6.1000
PRIMER_RIGHT_16_END_STABILITY=5.6000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=863
PRIMER_PAIR_PENALTY_17=1.3532
PRIMER_LEFT_17_PENALTY=0.966648
PRIMER_RIGHT_17_PENALTY=0.386599
PRIMER_LEFT_17_SEQUENCE=GAGTTTGAGAACATGTCGGTGAC
PRIMER_RIGHT_17_SEQUENCE=GTGCTGGTAGTCCCTCATGATTA
PRIMER_LEFT_17=471,23
PRIMER_RIGHT_17=1334,23
PRIMER_LEFT_17_TM=60.967
PRIMER_RIGHT_17_TM=60.387
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, MER34 a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=864
PRIMER_PAIR_PENALTY_18=1.3643
PRIMER_LEFT_18_PENALTY=1.006403
PRIMER_RIGHT_18_PENALTY=0.357879
PRIMER_LEFT_18_SEQUENCE=ATGTTCTTCTGTCTCCAGGAAGG
PRIMER_RIGHT_18_SEQUENCE=GCACACACTCATACATGTTCACC
PRIMER_LEFT_18=2239,23
PRIMER_RIGHT_18=2349,23
PRIMER_LEFT_18_TM=61.006
PRIMER_RIGHT_18_TM=60.358
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=6.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER39 a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=7.9000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=111
PRIMER_PAIR_PENALTY_19=1.3700
PRIMER_LEFT_19_PENALTY=0.357879
PRIMER_RIGHT_19_PENALTY=1.012153
PRIMER_LEFT_19_SEQUENCE=GGTGAACATGTATGAGTGTGTGC
PRIMER_RIGHT_19_SEQUENCE=CTAGAAAAGAGGAGGCTGTCCA
PRIMER_LEFT_19=2327,23
PRIMER_RIGHT_19=2620,22
PRIMER_LEFT_19_TM=60.358
PRIMER_RIGHT_19_TM=60.012
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=294
=