PRIMER_SEQUENCE_ID=NM_006281
SEQUENCE=CCGCGGAGTTACGGGAAAGTTGGTCCGAGTTCCCAGAGTTTCCCTCTGTGGTGCCCTAGGCTTCGGCCCGGTGCCCCGGCTCCTTTCCTCCTTTCGGCCTTCGCCGTCCACCAGGTCCCTCTCTCTGTCCCGGCCGCCATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAAGTTGTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCCATAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAATACAGACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGATTACGAAACAAGACATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGACTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAAAGACATGACGAACAGCAACGAGAATTGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGGAGAGTGTGGCACCATGCGGGCCACAAGCACGATGAGTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAGGAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATAAGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAGATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAACGGGAGATAGAAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTGAGTCTAATTTCCTCTCTGTTTTTAACTATTCTGGAGACCAAGAAACCACTAGGAATTGAAGGAATATTTGGATATTTTTAATCCTAAGATTTTGCCCTACAATTAGGCAGAGGTCAAAAAGTGACAATGGTACATGCCCAGGTAAATTCCCAAAAGGCAGAATTGACAGTTGTATCTGCTGTGCATTCACTCTAAGATGAGGAGAACAAAAGAAGTGTATTCTCTTGTTCTGTCAGCTGCATACCAGTAATAAAACTGTTATGAAATGGATTTTCAAGGTCTCTAAACCTTGAAAATCCAAAGGCTATTGTTGCATTGTACAGCACTGAAAGGGCTTTATGTTACAATATTCTTTATTCCTATCTAGTATACTAGGCTATTTATTGTCCCCTTAGGTAAACTTATTTATTTATGCTATTTTGGCTTTGTTTCATTTTTTAAGGACAAGATCAGGATAGCTTTGGTGAAGGTAGGGTCATATTAATATGATGATAATGTGCAACCAATTTATACTTTCTGCAGGGAGCTATGGGGTACATTCCTTGATTTCCAGGATAGTTTTTCAAATAGGAAAGCAATAATGGCAGTAGTTCTCAAATGGGCTAGGCCTTTTTTATATTGAAGCAATAATTCCATTTTTACCCTTTGAAATTTTGTTTTTTTGATTTTTGATGTTTGGTACAAATAGAACTATATATATTTAGGTAAAATAGATCTATCGTGTTTAAAACCAAAGAAATCAATGGAACCCTTGCACAAAAAAGTGTGATAAATATTTTTAAATAAAAACTTAATACAAATGTAATTTGTTAATATTGTTTCATGTTTTATGTGTAGATCTAATAGCTGAACTGATTCAAACTGTAATAAGCTCATCAATTTCATTTCTATGAAAATGTGCTCTGTTGTCACAGGATGTTTCTGTTGATTTTATTCATTTCCTGGGAATTGGTAAACATCATGTTCCTGATGATAACCCAGTAGCAAAAACATTTGTACTGAGTGGTACAAGCCTTGGGGACTGAAAAAAAAAAAAGATTAAAACCATTAAAAAGAAACTCATTTTTACGCTGAATGAACATTTATATGATTGCATTGGGACCAGTCATTTCCTAAGCTACATATGGCCATCTTGACAGTGTTTTTTCTTTTGTGTGTTTAATTATTATGTGTAAATCATAAAGACAAATAAATTTCACTGTGCCAC
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 31887, GC content failed 2137, low tm 18326, high tm 4800, high any compl 5, high end compl 33, high repeat similarity 46, long poly-x seq 108, ok 6432
PRIMER_RIGHT_EXPLAIN=considered 31701, GC content failed 2313, low tm 18528, high tm 4295, high any compl 3, high end compl 19, high repeat similarity 21, long poly-x seq 133, ok 6389
PRIMER_PAIR_EXPLAIN=considered 858, unacceptable product size 791, high any compl 1, high end compl 16, ok 50
PRIMER_PAIR_PENALTY=0.0396
PRIMER_LEFT_PENALTY=0.013634
PRIMER_RIGHT_PENALTY=0.026007
PRIMER_LEFT_SEQUENCE=CAAAGAAATTTCCATAATGCAGC
PRIMER_RIGHT_SEQUENCE=TCAGGATTCTTCACCAAACACTT
PRIMER_LEFT=341,23
PRIMER_RIGHT=928,23
PRIMER_LEFT_TM=59.986
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=34.783
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=588
PRIMER_PAIR_PENALTY_1=0.0738
PRIMER_LEFT_1_PENALTY=0.042918
PRIMER_RIGHT_1_PENALTY=0.030877
PRIMER_LEFT_1_SEQUENCE=TGTATCTGCTGTGCATTCACTCT
PRIMER_RIGHT_1_SEQUENCE=CCTACCTTCACCAAAGCTATCCT
PRIMER_LEFT_1=1783,23
PRIMER_RIGHT_1=2086,23
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=304
PRIMER_PAIR_PENALTY_2=0.0744
PRIMER_LEFT_2_PENALTY=0.026007
PRIMER_RIGHT_2_PENALTY=0.048359
PRIMER_LEFT_2_SEQUENCE=AAGTGTTTGGTGAAGAATCCTGA
PRIMER_RIGHT_2_SEQUENCE=AAGTTTTGCTGCCTTCTTTTCTT
PRIMER_LEFT_2=906,23
PRIMER_RIGHT_2=1609,23
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=59.952
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=34.783
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=704
PRIMER_PAIR_PENALTY_3=0.0833
PRIMER_LEFT_3_PENALTY=0.057286
PRIMER_RIGHT_3_PENALTY=0.026007
PRIMER_LEFT_3_SEQUENCE=TTTTATGATTCCCACAAATCCAC
PRIMER_RIGHT_3_SEQUENCE=TCAGGATTCTTCACCAAACACTT
PRIMER_LEFT_3=827,23
PRIMER_RIGHT_3=928,23
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=34.783
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=102
PRIMER_PAIR_PENALTY_4=0.1056
PRIMER_LEFT_4_PENALTY=0.057286
PRIMER_RIGHT_4_PENALTY=0.048359
PRIMER_LEFT_4_SEQUENCE=TTTTATGATTCCCACAAATCCAC
PRIMER_RIGHT_4_SEQUENCE=AAGTTTTGCTGCCTTCTTTTCTT
PRIMER_LEFT_4=827,23
PRIMER_RIGHT_4=1609,23
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.952
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=34.783
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=783
PRIMER_PAIR_PENALTY_5=0.1277
PRIMER_LEFT_5_PENALTY=0.096788
PRIMER_RIGHT_5_PENALTY=0.030877
PRIMER_LEFT_5_SEQUENCE=TGAACATAATAGCACGATGTTGG
PRIMER_RIGHT_5_SEQUENCE=CCTACCTTCACCAAAGCTATCCT
PRIMER_LEFT_5=1190,23
PRIMER_RIGHT_5=2086,23
PRIMER_LEFT_5_TM=59.903
PRIMER_RIGHT_5_TM=60.031
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=7.8000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=897
PRIMER_PAIR_PENALTY_6=0.1291
PRIMER_LEFT_6_PENALTY=0.042918
PRIMER_RIGHT_6_PENALTY=0.086226
PRIMER_LEFT_6_SEQUENCE=TGTATCTGCTGTGCATTCACTCT
PRIMER_RIGHT_6_SEQUENCE=ACCCTACCTTCACCAAAGCTATC
PRIMER_LEFT_6=1783,23
PRIMER_RIGHT_6=2088,23
PRIMER_LEFT_6_TM=59.957
PRIMER_RIGHT_6_TM=59.914
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_6_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=306
PRIMER_PAIR_PENALTY_7=0.1346
PRIMER_LEFT_7_PENALTY=0.048359
PRIMER_RIGHT_7_PENALTY=0.086226
PRIMER_LEFT_7_SEQUENCE=AAGAAAAGAAGGCAGCAAAACTT
PRIMER_RIGHT_7_SEQUENCE=ACCCTACCTTCACCAAAGCTATC
PRIMER_LEFT_7=1587,23
PRIMER_RIGHT_7=2088,23
PRIMER_LEFT_7_TM=59.952
PRIMER_RIGHT_7_TM=59.914
PRIMER_LEFT_7_GC_PERCENT=34.783
PRIMER_RIGHT_7_GC_PERCENT=47.826
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_7_END_STABILITY=6.7000
PRIMER_RIGHT_7_END_STABILITY=5.6000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=502
PRIMER_PAIR_PENALTY_8=0.1387
PRIMER_LEFT_8_PENALTY=0.004943
PRIMER_RIGHT_8_PENALTY=0.133805
PRIMER_LEFT_8_SEQUENCE=CAGACCTCTGGATTGTTATGGAG
PRIMER_RIGHT_8_SEQUENCE=AGCATAAGGAGGTTTTCCTTCAG
PRIMER_LEFT_8=415,23
PRIMER_RIGHT_8=803,23
PRIMER_LEFT_8_TM=60.005
PRIMER_RIGHT_8_TM=60.134
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.0000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=389
PRIMER_PAIR_PENALTY_9=0.1451
PRIMER_LEFT_9_PENALTY=0.096788
PRIMER_RIGHT_9_PENALTY=0.048359
PRIMER_LEFT_9_SEQUENCE=TGAACATAATAGCACGATGTTGG
PRIMER_RIGHT_9_SEQUENCE=AAGTTTTGCTGCCTTCTTTTCTT
PRIMER_LEFT_9=1190,23
PRIMER_RIGHT_9=1609,23
PRIMER_LEFT_9_TM=59.903
PRIMER_RIGHT_9_TM=59.952
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=34.783
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_9_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=420
PRIMER_PAIR_PENALTY_10=0.1474
PRIMER_LEFT_10_PENALTY=0.013634
PRIMER_RIGHT_10_PENALTY=0.133805
PRIMER_LEFT_10_SEQUENCE=CAAAGAAATTTCCATAATGCAGC
PRIMER_RIGHT_10_SEQUENCE=AGCATAAGGAGGTTTTCCTTCAG
PRIMER_LEFT_10=341,23
PRIMER_RIGHT_10=803,23
PRIMER_LEFT_10_TM=59.986
PRIMER_RIGHT_10_TM=60.134
PRIMER_LEFT_10_GC_PERCENT=34.783
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=8.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, L1MA10 3'-end of L1 repeat (subfamily L1MA10) - a consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=9.7000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=463
PRIMER_PAIR_PENALTY_11=0.1481
PRIMER_LEFT_11_PENALTY=0.122120
PRIMER_RIGHT_11_PENALTY=0.026007
PRIMER_LEFT_11_SEQUENCE=ACAGACCTCTGGATTGTTATGGA
PRIMER_RIGHT_11_SEQUENCE=TCAGGATTCTTCACCAAACACTT
PRIMER_LEFT_11=414,23
PRIMER_RIGHT_11=928,23
PRIMER_LEFT_11_TM=59.878
PRIMER_RIGHT_11_TM=60.026
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=515
PRIMER_PAIR_PENALTY_12=0.1570
PRIMER_LEFT_12_PENALTY=0.108608
PRIMER_RIGHT_12_PENALTY=0.048359
PRIMER_LEFT_12_SEQUENCE=TTTATCAAGAATGCCAAACCTGT
PRIMER_RIGHT_12_SEQUENCE=AAGTTTTGCTGCCTTCTTTTCTT
PRIMER_LEFT_12=966,23
PRIMER_RIGHT_12=1609,23
PRIMER_LEFT_12_TM=59.891
PRIMER_RIGHT_12_TM=59.952
PRIMER_LEFT_12_GC_PERCENT=34.783
PRIMER_RIGHT_12_GC_PERCENT=34.783
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=3.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, LTR11 LTR from human HTLV-I related endogenous retroviral sequence (HRES-1/1)
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_12_MISPRIMING_SCORE=23.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=644
PRIMER_PAIR_PENALTY_13=0.1593
PRIMER_LEFT_13_PENALTY=0.133335
PRIMER_RIGHT_13_PENALTY=0.026007
PRIMER_LEFT_13_SEQUENCE=ATAGAAATGGCTGAAGGAAAACC
PRIMER_RIGHT_13_SEQUENCE=TCAGGATTCTTCACCAAACACTT
PRIMER_LEFT_13=771,23
PRIMER_RIGHT_13=928,23
PRIMER_LEFT_13_TM=59.867
PRIMER_RIGHT_13_TM=60.026
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=39.130
PRIMER_LEFT_13_SELF_ANY=2.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=6.7000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=158
PRIMER_PAIR_PENALTY_14=0.1650
PRIMER_LEFT_14_PENALTY=0.026007
PRIMER_RIGHT_14_PENALTY=0.138962
PRIMER_LEFT_14_SEQUENCE=AAGTGTTTGGTGAAGAATCCTGA
PRIMER_RIGHT_14_SEQUENCE=AGTGCTTTTAACCGCATCTGTAG
PRIMER_LEFT_14=906,23
PRIMER_RIGHT_14=1501,23
PRIMER_LEFT_14_TM=60.026
PRIMER_RIGHT_14_TM=59.861
PRIMER_LEFT_14_GC_PERCENT=39.130
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=2.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=596
PRIMER_PAIR_PENALTY_15=0.1703
PRIMER_LEFT_15_PENALTY=0.144318
PRIMER_RIGHT_15_PENALTY=0.026007
PRIMER_LEFT_15_SEQUENCE=CTCCTCAATACAGAAGGACATGC
PRIMER_RIGHT_15_SEQUENCE=TCAGGATTCTTCACCAAACACTT
PRIMER_LEFT_15=594,23
PRIMER_RIGHT_15=928,23
PRIMER_LEFT_15_TM=60.144
PRIMER_RIGHT_15_TM=60.026
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.4000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=335
PRIMER_PAIR_PENALTY_16=0.1714
PRIMER_LEFT_16_PENALTY=0.032476
PRIMER_RIGHT_16_PENALTY=0.138962
PRIMER_LEFT_16_SEQUENCE=AGCTAAAAGACATGACGAACAGC
PRIMER_RIGHT_16_SEQUENCE=AGTGCTTTTAACCGCATCTGTAG
PRIMER_LEFT_16=1031,23
PRIMER_RIGHT_16=1501,23
PRIMER_LEFT_16_TM=59.968
PRIMER_RIGHT_16_TM=59.861
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1PA2 3'-end of L1 repeat (subfamily L1PA2) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MIR mammalian-wide interspersed repeat (MIR) - a consensus
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_16_END_STABILITY=7.9000
PRIMER_RIGHT_16_END_STABILITY=5.7000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=471
PRIMER_PAIR_PENALTY_17=0.1718
PRIMER_LEFT_17_PENALTY=0.123491
PRIMER_RIGHT_17_PENALTY=0.048359
PRIMER_LEFT_17_SEQUENCE=CCATGGTGATAAACAGTGAGGAT
PRIMER_RIGHT_17_SEQUENCE=AAGTTTTGCTGCCTTCTTTTCTT
PRIMER_LEFT_17=1228,23
PRIMER_RIGHT_17=1609,23
PRIMER_LEFT_17_TM=60.123
PRIMER_RIGHT_17_TM=59.952
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=34.783
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.8000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=382
PRIMER_PAIR_PENALTY_18=0.1830
PRIMER_LEFT_18_PENALTY=0.096788
PRIMER_RIGHT_18_PENALTY=0.086226
PRIMER_LEFT_18_SEQUENCE=TGAACATAATAGCACGATGTTGG
PRIMER_RIGHT_18_SEQUENCE=ACCCTACCTTCACCAAAGCTATC
PRIMER_LEFT_18=1190,23
PRIMER_RIGHT_18=2088,23
PRIMER_LEFT_18_TM=59.903
PRIMER_RIGHT_18_TM=59.914
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=5.6000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=899
PRIMER_PAIR_PENALTY_19=0.1846
PRIMER_LEFT_19_PENALTY=0.004943
PRIMER_RIGHT_19_PENALTY=0.179693
PRIMER_LEFT_19_SEQUENCE=CAGACCTCTGGATTGTTATGGAG
PRIMER_RIGHT_19_SEQUENCE=ATTACAGTATTGCGTTTTGCCAT
PRIMER_LEFT_19=415,23
PRIMER_RIGHT_19=682,23
PRIMER_LEFT_19_TM=60.005
PRIMER_RIGHT_19_TM=59.820
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=34.783
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=9.6000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=268
=