PRIMER_SEQUENCE_ID=NM_007181
SEQUENCE=GTCTTTATTTCAGTCCCGGATCCGCGGGCGCAGGCCCAGCTCAGGCCCCCAGGGATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGGGTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGATGGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGGCACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCTGTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGTCTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGAGCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGGCTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGCCCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAGCTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGCCTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAAGAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAATCGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTGAGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCTGGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGACCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGTCAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACTTCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTGAGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCATCCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGACAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTGTTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACCTGCTCCTGGGGGCAGAGGAAGGCATCTTCATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCTCTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCCCACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTCACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTGCCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCCGTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGCCGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGTGAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTGGGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGTTGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGATCCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGAGTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTCTGCTTGGCTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCGCCTCACTGCAACCTCCTCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCCTGTAGTGGTGGAGACACGCCCAGTGGATGATCCTACTGCTCCCAGCAACCTCTACATCCAGGAATGAGTCCCTAGGGGGGTGTCAGGAACTAGTCCTTGCACCCCCTCCCCCATAGACACACTAGTGGTCATGGCATGTCCTCATCTCCCAATAAACATGACTTTAGCCTCTGCAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 30936, GC content failed 555, low tm 7525, high tm 16701, high any compl 13, high end compl 29, high repeat similarity 63, long poly-x seq 84, ok 5966
PRIMER_RIGHT_EXPLAIN=considered 30925, GC content failed 395, low tm 7756, high tm 16512, high any compl 4, high end compl 5, high repeat similarity 19, long poly-x seq 97, ok 6137
PRIMER_PAIR_EXPLAIN=considered 472, unacceptable product size 427, high end compl 3, ok 42
PRIMER_PAIR_PENALTY=0.1350
PRIMER_LEFT_PENALTY=0.130037
PRIMER_RIGHT_PENALTY=0.004943
PRIMER_LEFT_SEQUENCE=CCCACCTGTATTCTCATAGCATC
PRIMER_RIGHT_SEQUENCE=CTCATTCCTGGATGTAGAGGTTG
PRIMER_LEFT=1732,23
PRIMER_RIGHT=2618,23
PRIMER_LEFT_TM=59.870
PRIMER_RIGHT_TM=60.005
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=20.00, L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=887
PRIMER_PAIR_PENALTY_1=0.1711
PRIMER_LEFT_1_PENALTY=0.122120
PRIMER_RIGHT_1_PENALTY=0.048962
PRIMER_LEFT_1_SEQUENCE=GAAGATACACAGGGACATCAAGG
PRIMER_RIGHT_1_SEQUENCE=GGTCGTTTCTTGGGACTCTTAGT
PRIMER_LEFT_1=449,23
PRIMER_RIGHT_1=841,23
PRIMER_LEFT_1_TM=59.878
PRIMER_RIGHT_1_TM=60.049
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=393
PRIMER_PAIR_PENALTY_2=0.1751
PRIMER_LEFT_2_PENALTY=0.170187
PRIMER_RIGHT_2_PENALTY=0.004943
PRIMER_LEFT_2_SEQUENCE=CTGAGAGACCCTACCCTCACTTT
PRIMER_RIGHT_2_SEQUENCE=CTCATTCCTGGATGTAGAGGTTG
PRIMER_LEFT_2=2406,23
PRIMER_RIGHT_2=2618,23
PRIMER_LEFT_2_TM=60.170
PRIMER_RIGHT_2_TM=60.005
PRIMER_LEFT_2_GC_PERCENT=52.174
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=11.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=20.00, SVA Composite retroposon
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.2138
PRIMER_LEFT_3_PENALTY=0.208880
PRIMER_RIGHT_3_PENALTY=0.004943
PRIMER_LEFT_3_SEQUENCE=GACCCAGGTAGAGGAAGATATGG
PRIMER_RIGHT_3_SEQUENCE=CTCATTCCTGGATGTAGAGGTTG
PRIMER_LEFT_3=2192,23
PRIMER_RIGHT_3=2618,23
PRIMER_LEFT_3_TM=60.209
PRIMER_RIGHT_3_TM=60.005
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=20.00, Tigger2 Autonomous DNA transposon
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=427
PRIMER_PAIR_PENALTY_4=0.3057
PRIMER_LEFT_4_PENALTY=0.175636
PRIMER_RIGHT_4_PENALTY=0.130037
PRIMER_LEFT_4_SEQUENCE=TCTCGTAAAGTTGTTCAATGGCT
PRIMER_RIGHT_4_SEQUENCE=GGATGCTATGAGAATACAGGTGG
PRIMER_LEFT_4=1511,23
PRIMER_RIGHT_4=1755,23
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
PRIMER_PAIR_PENALTY_5=0.3389
PRIMER_LEFT_5_PENALTY=0.130037
PRIMER_RIGHT_5_PENALTY=0.208880
PRIMER_LEFT_5_SEQUENCE=CCCACCTGTATTCTCATAGCATC
PRIMER_RIGHT_5_SEQUENCE=CCATATCTTCCTCTACCTGGGTC
PRIMER_LEFT_5=1732,23
PRIMER_RIGHT_5=2214,23
PRIMER_LEFT_5_TM=59.870
PRIMER_RIGHT_5_TM=60.209
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=52.174
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=9.1000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=483
PRIMER_PAIR_PENALTY_6=0.3517
PRIMER_LEFT_6_PENALTY=0.346766
PRIMER_RIGHT_6_PENALTY=0.004943
PRIMER_LEFT_6_SEQUENCE=CATCAACAACGTTCTCATGTCTC
PRIMER_RIGHT_6_SEQUENCE=CTCATTCCTGGATGTAGAGGTTG
PRIMER_LEFT_6=1694,23
PRIMER_RIGHT_6=2618,23
PRIMER_LEFT_6_TM=59.653
PRIMER_RIGHT_6_TM=60.005
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_6_END_STABILITY=6.1000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=925
PRIMER_PAIR_PENALTY_7=0.3845
PRIMER_LEFT_7_PENALTY=0.175636
PRIMER_RIGHT_7_PENALTY=0.208880
PRIMER_LEFT_7_SEQUENCE=TCTCGTAAAGTTGTTCAATGGCT
PRIMER_RIGHT_7_SEQUENCE=CCATATCTTCCTCTACCTGGGTC
PRIMER_LEFT_7=1511,23
PRIMER_RIGHT_7=2214,23
PRIMER_LEFT_7_TM=60.176
PRIMER_RIGHT_7_TM=60.209
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=52.174
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=9.1000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=704
PRIMER_PAIR_PENALTY_8=0.4032
PRIMER_LEFT_8_PENALTY=0.122120
PRIMER_RIGHT_8_PENALTY=0.281096
PRIMER_LEFT_8_SEQUENCE=GAAGATACACAGGGACATCAAGG
PRIMER_RIGHT_8_SEQUENCE=TCGTTTCTTGGGACTCTTAGTCA
PRIMER_LEFT_8=449,23
PRIMER_RIGHT_8=839,23
PRIMER_LEFT_8_TM=59.878
PRIMER_RIGHT_8_TM=60.281
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=3.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.5000
PRIMER_RIGHT_8_END_STABILITY=6.4000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=391
PRIMER_PAIR_PENALTY_9=0.4111
PRIMER_LEFT_9_PENALTY=0.281096
PRIMER_RIGHT_9_PENALTY=0.130037
PRIMER_LEFT_9_SEQUENCE=TGACTAAGAGTCCCAAGAAACGA
PRIMER_RIGHT_9_SEQUENCE=GGATGCTATGAGAATACAGGTGG
PRIMER_LEFT_9=817,23
PRIMER_RIGHT_9=1755,23
PRIMER_LEFT_9_TM=60.281
PRIMER_RIGHT_9_TM=59.870
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=8.4000
PRIMER_RIGHT_9_END_STABILITY=9.4000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=939
PRIMER_PAIR_PENALTY_10=0.4320
PRIMER_LEFT_10_PENALTY=0.130037
PRIMER_RIGHT_10_PENALTY=0.301915
PRIMER_LEFT_10_SEQUENCE=CCCACCTGTATTCTCATAGCATC
PRIMER_RIGHT_10_SEQUENCE=TTGCAGAGGCTAAAGTCATGTTT
PRIMER_LEFT_10=1732,23
PRIMER_RIGHT_10=2726,23
PRIMER_LEFT_10_TM=59.870
PRIMER_RIGHT_10_TM=60.302
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=11.00, MSTc consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=8.1000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=995
PRIMER_PAIR_PENALTY_11=0.4320
PRIMER_LEFT_11_PENALTY=0.130037
PRIMER_RIGHT_11_PENALTY=0.301915
PRIMER_LEFT_11_SEQUENCE=CCCACCTGTATTCTCATAGCATC
PRIMER_RIGHT_11_SEQUENCE=TTTGCAGAGGCTAAAGTCATGTT
PRIMER_LEFT_11=1732,23
PRIMER_RIGHT_11=2727,23
PRIMER_LEFT_11_TM=59.870
PRIMER_RIGHT_11_TM=60.302
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=11.00, LTR6 LTR from  human SSAV-related endogenous retroviral LTR-like element
PRIMER_PAIR_11_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_11_END_STABILITY=8.1000
PRIMER_RIGHT_11_END_STABILITY=6.6000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=996
PRIMER_PAIR_PENALTY_12=0.4320
PRIMER_LEFT_12_PENALTY=0.130037
PRIMER_RIGHT_12_PENALTY=0.301915
PRIMER_LEFT_12_SEQUENCE=CCCACCTGTATTCTCATAGCATC
PRIMER_RIGHT_12_SEQUENCE=TTTTGCAGAGGCTAAAGTCATGT
PRIMER_LEFT_12=1732,23
PRIMER_RIGHT_12=2728,23
PRIMER_LEFT_12_TM=59.870
PRIMER_RIGHT_12_TM=60.302
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=39.130
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=8.1000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=997
PRIMER_PAIR_PENALTY_13=0.4457
PRIMER_LEFT_13_PENALTY=0.122120
PRIMER_RIGHT_13_PENALTY=0.323594
PRIMER_LEFT_13_SEQUENCE=GAAGATACACAGGGACATCAAGG
PRIMER_RIGHT_13_SEQUENCE=TGTCACACAGCTCATTGTATCCT
PRIMER_LEFT_13=449,23
PRIMER_RIGHT_13=645,23
PRIMER_LEFT_13_TM=59.878
PRIMER_RIGHT_13_TM=59.676
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=3.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, L1PA15 3'-end of L1 repeat (subfamily L1PA15) - a consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=7.8000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=197
PRIMER_PAIR_PENALTY_14=0.4643
PRIMER_LEFT_14_PENALTY=0.415358
PRIMER_RIGHT_14_PENALTY=0.048962
PRIMER_LEFT_14_SEQUENCE=AGTCTTTAAGGCTCGAGACAAGG
PRIMER_RIGHT_14_SEQUENCE=GGTCGTTTCTTGGGACTCTTAGT
PRIMER_LEFT_14=143,23
PRIMER_RIGHT_14=841,23
PRIMER_LEFT_14_TM=60.415
PRIMER_RIGHT_14_TM=60.049
PRIMER_LEFT_14_GC_PERCENT=47.826
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=3.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=3.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.5000
PRIMER_RIGHT_14_END_STABILITY=5.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=699
PRIMER_PAIR_PENALTY_15=0.4776
PRIMER_LEFT_15_PENALTY=0.472654
PRIMER_RIGHT_15_PENALTY=0.004943
PRIMER_LEFT_15_SEQUENCE=TGTATTCTCATAGCATCCTTGGC
PRIMER_RIGHT_15_SEQUENCE=CTCATTCCTGGATGTAGAGGTTG
PRIMER_LEFT_15=1738,23
PRIMER_RIGHT_15=2618,23
PRIMER_LEFT_15_TM=60.473
PRIMER_RIGHT_15_TM=60.005
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1a (MLT1a subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=10.0000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=881
PRIMER_PAIR_PENALTY_16=0.5069
PRIMER_LEFT_16_PENALTY=0.376837
PRIMER_RIGHT_16_PENALTY=0.130037
PRIMER_LEFT_16_SEQUENCE=TTCTTGACAAACTGAAGAATCCC
PRIMER_RIGHT_16_SEQUENCE=GGATGCTATGAGAATACAGGTGG
PRIMER_LEFT_16=913,23
PRIMER_RIGHT_16=1755,23
PRIMER_LEFT_16_TM=59.623
PRIMER_RIGHT_16_TM=59.870
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=9.3000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_16=843
PRIMER_PAIR_PENALTY_17=0.5108
PRIMER_LEFT_17_PENALTY=0.208880
PRIMER_RIGHT_17_PENALTY=0.301915
PRIMER_LEFT_17_SEQUENCE=GACCCAGGTAGAGGAAGATATGG
PRIMER_RIGHT_17_SEQUENCE=TTTGCAGAGGCTAAAGTCATGTT
PRIMER_LEFT_17=2192,23
PRIMER_RIGHT_17=2727,23
PRIMER_LEFT_17_TM=60.209
PRIMER_RIGHT_17_TM=60.302
PRIMER_LEFT_17_GC_PERCENT=52.174
PRIMER_RIGHT_17_GC_PERCENT=39.130
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=11.00, LTR6 LTR from  human SSAV-related endogenous retroviral LTR-like element
PRIMER_PAIR_17_MISPRIMING_SCORE=20.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=7.4000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=536
PRIMER_PAIR_PENALTY_18=0.5108
PRIMER_LEFT_18_PENALTY=0.208880
PRIMER_RIGHT_18_PENALTY=0.301915
PRIMER_LEFT_18_SEQUENCE=GACCCAGGTAGAGGAAGATATGG
PRIMER_RIGHT_18_SEQUENCE=TTGCAGAGGCTAAAGTCATGTTT
PRIMER_LEFT_18=2192,23
PRIMER_RIGHT_18=2726,23
PRIMER_LEFT_18_TM=60.209
PRIMER_RIGHT_18_TM=60.302
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MSTc consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.4000
PRIMER_RIGHT_18_END_STABILITY=7.0000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=535
PRIMER_PAIR_PENALTY_19=0.5108
PRIMER_LEFT_19_PENALTY=0.208880
PRIMER_RIGHT_19_PENALTY=0.301915
PRIMER_LEFT_19_SEQUENCE=GACCCAGGTAGAGGAAGATATGG
PRIMER_RIGHT_19_SEQUENCE=TTTTGCAGAGGCTAAAGTCATGT
PRIMER_LEFT_19=2192,23
PRIMER_RIGHT_19=2728,23
PRIMER_LEFT_19_TM=60.209
PRIMER_RIGHT_19_TM=60.302
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MER11A a consensus
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, L1MC2 3'-end of L1 repeat (subfamily L1MC2) - a consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.4000
PRIMER_RIGHT_19_END_STABILITY=6.6000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=537
=