PRIMER_SEQUENCE_ID=NM_016151
SEQUENCE=GAGACCGGGACGAGACCGGGGCTGTGGTGCGGAGAGAGGCTGAGACGGAGAAGAGGAGAGGCAGAGAGGGCGCGGGGACCGTCAGCAGCACCTTAGCTACAATCGTTCAGCTATTCTCGGAAGAGAGAAGGGAGAGGGAGGAGGCCGGGGCGGGAGTGGGGGCTGTCACCCTCGGACCCCGGCGTGAGAGGGGCCGTGCGGCCGGACGTCCTCGGGGTGGGCCCCCAGTCGGTGGCCGAAGACCTACAGCTCAGGCCCCTGGGTCCCAAATTTCCAGGCTTTGCCCCTCCTCCTTTCTCAGATACCCGGGTAACAGTCCTCATAGTCCAGATATCCGGGACTCGGGTCCCAACCTCTCTAAACCTGGGTCTCTGTTTCATAGAATTTCAAATATCAGGTTCAGGCCCCTGCGTGCACCAGTATCCGGGGTTCATTCCCCGGGCGTTCAAATATCGGATTCAGTCTCCATCCCGTTCAGATATTCGGGGTTCAGACCCCACAATCAGAAATCCGGAATTCGGCAGCTGTCGCCCTCGACGAGGGGGAGGACTGGACCGCGAGGTCAGATTAGGTTGTCACCCCCTCCCCTCCAGGGGAGGCTTCCCGGGCCCGCCCCTCAGGAAGGGCGAAAGCCGAGGAAGAGGTGGCAAGGGGAAAGGTCTCCTTGCCCCTCTCCCTGCTTGGCAGAGCCGCTGGAGGACCCCAGGCGGAAGCGGAGGCGCTGGGGCACCATAGTGACCCCTACCAGGCCAGGCCCCACTCTCAGGGCCCCCAGGGGCCACCATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACCCAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGATGTCCGGAATAGTGAGGTGGTGGCCATCAAGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGGCAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGGGGCTGTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTTCTAGAAGTGCACAAGAAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGCCTGGCATATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCAGAGCCAGGGTTAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAACTCCTTCGTGGGCACCCCATACTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGGCAGTACGATGGCAAAGTGGACGTCTGGTCCTTGGGGATAACCTGCATCGAGCTGGCTGAACGGAAACCACCGCTCTTTAACATGAATGCGATGAGTGCCTTATACCACATTGCACAGAACGAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTTTGTCGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTGAAGCACCGCTTTGTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGATGCCGTGCGGGAGCTGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCACCCAACGGCCCTGGTGCCGAGGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCACCGGGCCGGGACTCTGACCAGCCTCGAGAGTAGCCACTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCCGTCAACAGCCTAGCAGATGCCTCAGACAACGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGGCCCTGAAGCCCGGGAGATGGCCATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCCATTATCCACCGGCTGCCGGGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGCCCTCTCCAGCCGCCTGCAGCCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGCCGTAACCGAGACCACTTTGCCACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAGCAGGACTCTGCGCTGCGGGAGCAGCTGAGCGGCTATAAGCGGATGCGACGACAGCACCAGAAGCAGCTGCTGGCCCTGGAGTCACGGCTGAGGGGTGAACGGGAGGAGCACAGTGCACGGCTGCAGCGGGAGCTTGAGGCGCAGCGGGCTGGCTTTGGGGCAGAGGCAGAAAAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAGGAGGCACGAGCTGCCCAGGCCGAGGAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAGCTGGCTGCCCTGCTGGAGGCACAGAAGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTCCAGGAGAACCCCAGCACTCCCAAGCGGGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTCCAGCAGTGCCAGGCGGAGGAGGAAGCAGGGCTGCTGCGGCGGCAGCGCCAGTACTTTGAGCTGCAGTGTCGCCAGTACAAGCGCAAGATGTTGCTGGCTCGGCACAGCCTGGACCAGGACCTGCTGCGGGAGGACCTGAACAAGAAGCAGACCCAGAAGGACTTGGAGTGTGCACTGCTGCTTCGGCAGCACGAGGCCACGCGGGAGCTGGAGCTGCGGCAGCTCCAGGCCGTGCAGCGCACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAGCTGGGCAACCAGCTGGAGTACAACAAGCGGCGTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTTCGCCAGCAGCCCAAGAGCCTCAAAGTACGTGCAGGCCAGCGCCCCCCGGGCCTTCCACTCCCCATTCCTGGGGCTCTGGGCCCACCCAACACAGGCACCCCTATAGAACAGCAGCCCTGCTCACCTGGCCAGGAGGCAGTCCTGGACCAAAGAATGCTTGGCGAGGAGGAGGAAGCAGTTGGAGAGAGAAGGATTCTGGGAAAGGAAGGGGCCACTTTGGAGCCCAAGCAGCAGAGGATTCTGGGGGAAGAATCAGGAGCCCCTAGTCCCAGTCCACAAAAACATGGGAGCCTGGTTGATGAGGAAGTTTGGGGTCTGCCTGAGGAGATAGAGGAGCTTAGGGTGCCCTCCCTTGTACCCCAGGAGAGGAGCATTGTTGGCCAGGAGGAGGCTGGGACATGGAGCTTGTGGGGGAAGGAGGATGAGAGTCTTCTGGATGAGGAGTTTGAGCTTGGCTGGGTCCAGGGCCCAGCACTGACTCCCGTCCCTGAGGAGGAGGAAGAAGAGGAAGAGGGGGCTCCGATTGGGACCCCTAGGGATCCTGGAGATGGTTGTCCTTCCCCCGACATCCCTCCTGAACCCCCTCCAACACACCTGAGGCCCTGCCCTGCCAGCCAGCTCCCTGGACTCCTGTCCCATGGCCTCCTGGCCGGCCTCTCCTTTGCAGTGGGGTCCTCCTCTGGCCTCCTGCCCCTCCTGCTGCTGCTGCTGCTTCCATTGCTGGCAGCCCAGGGTGGGGGTGGCCTGCAGGCAGCGCTGCTGGCCCTTGAGGTGGGGCTGGTGGGTCTGGGGGCCTCCTACCTGCTCCTTTGTACAGCCCTGCACCTGCCCTCCAGTCTTTTCCTACTCCTGGCCCAGGGTACCGCACTGGGGGCCGTCCTGGGCCTGAGCTGGCGCCGAGGCCTCATGGGTGTTCCCCTGGGCCTTGGAGCTGCCTGGCTCTTAGCTTGGCCAGGCCTAGCTCTACCTCTGGTGGCTATGGCAGCGGGGGGCAGATGGGTGCGGCAGCAGGGCCCCCGGGTGCGCCGGGGCATATCTCGACTCTGGTTGCGGGTTCTGCTGCGCCTGTCACCCATGGCCTTCCGGGCCCTGCAGGGCTGTGGGGCTGTGGGGGACCGGGGTCTGTTTGCACTGTACCCCAAAACCAACAAGGATGGCTTCCGCAGCCGCCTGCCCGTCCCTGGGCCCCGGCGGCGTAATCCCCGCACCACCCAACACCCATTAGCTCTGTTGGCAAGGGTCTGGGTCCTGTGCAAGGGCTGGAACTGGCGTCTGGCACGGGCCAGCCAGGGTTTAGCATCCCACTTGCCCCCGTGGGCCATCCACACACTGGCCAGCTGGGGCCTGCTTCGGGGTGAACGGCCCACCCGAATCCCCCGGCTACTACCACGCAGCCAGCGCCAGCTAGGGCCCCCTGCCTCCCGCCAGCCACTGCCAGGGACTCTAGCCGGGCGGAGGTCACGCACCCGCCAGTCCCGGGCCCTGCCCCCCTGGAGGTAGCTGACTCCAGCCCTTCCAGCCCAAATCTAGAGCATTGAGCACTTTATCTCCCACGACTCAGTGAAGTTTCTCCAGTCCCTAGTCCTCTCTTTTCACCCACCTTCCTCAGTTTGCTCACTTACCCCAGGCCCAGCCCTTCGGACCTCTAGACAGGCAGCCTCCTCAGCTGTGGAGTCCAGCAGTCACTCTGTGTTCTCCTGGCGCTCCTCCCCTAAGTTATTGCTGTTCGCCCGCTGTGTGTGCTCATCCTCACCCTCATTGACTCAGGCCTGGGGCCAGGGGTGGTGGAGGGTGGGAAGAGTCATGTTTTTTTTCTCCTCTTTGATTTTGTTTTTCTGTCTCCCTTCCAACCTGTCCCCTTCCCCCCACCAAAAAAAGAAAAAGACAAACACAAATAAAATATCTGAGCGGAACTGTGAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 57336, GC content failed 2626, low tm 11531, high tm 32857, high any compl 1, high end compl 11, high repeat similarity 24, long poly-x seq 75, ok 10211
PRIMER_RIGHT_EXPLAIN=considered 57039, GC content failed 2670, low tm 11696, high tm 32344, high any compl 2, high end compl 13, high repeat similarity 10, long poly-x seq 120, ok 10184
PRIMER_PAIR_EXPLAIN=considered 10606, unacceptable product size 10540, high end compl 13, ok 53
PRIMER_PAIR_PENALTY=0.0635
PRIMER_LEFT_PENALTY=0.032614
PRIMER_RIGHT_PENALTY=0.030877
PRIMER_LEFT_SEQUENCE=ACGACTCAGTGAAGTTTCTCCAG
PRIMER_RIGHT_SEQUENCE=ACAGCAATAACTTAGGGGAGGAG
PRIMER_LEFT=4543,23
PRIMER_RIGHT=4716,23
PRIMER_LEFT_TM=59.967
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.1045
PRIMER_LEFT_1_PENALTY=0.073619
PRIMER_RIGHT_1_PENALTY=0.030877
PRIMER_LEFT_1_SEQUENCE=CCAAATCTAGAGCATTGAGCACT
PRIMER_RIGHT_1_SEQUENCE=ACAGCAATAACTTAGGGGAGGAG
PRIMER_LEFT_1=4511,23
PRIMER_RIGHT_1=4716,23
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MER3 Nonautonomous DNA transposon
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.1735
PRIMER_LEFT_2_PENALTY=0.030877
PRIMER_RIGHT_2_PENALTY=0.142611
PRIMER_LEFT_2_SEQUENCE=CTCCTCCCCTAAGTTATTGCTGT
PRIMER_RIGHT_2_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_2=4694,23
PRIMER_RIGHT_2=4909,23
PRIMER_LEFT_2_TM=60.031
PRIMER_RIGHT_2_TM=60.143
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=39.130
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.1735
PRIMER_LEFT_3_PENALTY=0.030877
PRIMER_RIGHT_3_PENALTY=0.142611
PRIMER_LEFT_3_SEQUENCE=CTCCTCCCCTAAGTTATTGCTGT
PRIMER_RIGHT_3_SEQUENCE=TTTCACAGTTCCGCTCAGATATT
PRIMER_LEFT_3=4694,23
PRIMER_RIGHT_3=4911,23
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.143
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.1752
PRIMER_LEFT_4_PENALTY=0.032614
PRIMER_RIGHT_4_PENALTY=0.142611
PRIMER_LEFT_4_SEQUENCE=ACGACTCAGTGAAGTTTCTCCAG
PRIMER_RIGHT_4_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_4=4543,23
PRIMER_RIGHT_4=4909,23
PRIMER_LEFT_4_TM=59.967
PRIMER_RIGHT_4_TM=60.143
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=367
PRIMER_PAIR_PENALTY_5=0.1752
PRIMER_LEFT_5_PENALTY=0.032614
PRIMER_RIGHT_5_PENALTY=0.142611
PRIMER_LEFT_5_SEQUENCE=ACGACTCAGTGAAGTTTCTCCAG
PRIMER_RIGHT_5_SEQUENCE=TTTCACAGTTCCGCTCAGATATT
PRIMER_LEFT_5=4543,23
PRIMER_RIGHT_5=4911,23
PRIMER_LEFT_5_TM=59.967
PRIMER_RIGHT_5_TM=60.143
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=39.130
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=5.8000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=369
PRIMER_PAIR_PENALTY_6=0.2162
PRIMER_LEFT_6_PENALTY=0.073619
PRIMER_RIGHT_6_PENALTY=0.142611
PRIMER_LEFT_6_SEQUENCE=CCAAATCTAGAGCATTGAGCACT
PRIMER_RIGHT_6_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_6=4511,23
PRIMER_RIGHT_6=4909,23
PRIMER_LEFT_6_TM=59.926
PRIMER_RIGHT_6_TM=60.143
PRIMER_LEFT_6_GC_PERCENT=43.478
PRIMER_RIGHT_6_GC_PERCENT=39.130
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, MER3 Nonautonomous DNA transposon
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.9000
PRIMER_RIGHT_6_END_STABILITY=7.2000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=399
PRIMER_PAIR_PENALTY_7=0.2162
PRIMER_LEFT_7_PENALTY=0.073619
PRIMER_RIGHT_7_PENALTY=0.142611
PRIMER_LEFT_7_SEQUENCE=CCAAATCTAGAGCATTGAGCACT
PRIMER_RIGHT_7_SEQUENCE=TTTCACAGTTCCGCTCAGATATT
PRIMER_LEFT_7=4511,23
PRIMER_RIGHT_7=4911,23
PRIMER_LEFT_7_TM=59.926
PRIMER_RIGHT_7_TM=60.143
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=39.130
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MER3 Nonautonomous DNA transposon
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=5.8000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=401
PRIMER_PAIR_PENALTY_8=0.2468
PRIMER_LEFT_8_PENALTY=0.104238
PRIMER_RIGHT_8_PENALTY=0.142611
PRIMER_LEFT_8_SEQUENCE=TCTCCAGTCCCTAGTCCTCTCTT
PRIMER_RIGHT_8_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_8=4559,23
PRIMER_RIGHT_8=4909,23
PRIMER_LEFT_8_TM=59.896
PRIMER_RIGHT_8_TM=60.143
PRIMER_LEFT_8_GC_PERCENT=52.174
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.7000
PRIMER_RIGHT_8_END_STABILITY=7.2000
PRIMER_PAIR_8_COMPL_ANY=3.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=351
PRIMER_PAIR_PENALTY_9=0.2468
PRIMER_LEFT_9_PENALTY=0.104238
PRIMER_RIGHT_9_PENALTY=0.142611
PRIMER_LEFT_9_SEQUENCE=TCTCCAGTCCCTAGTCCTCTCTT
PRIMER_RIGHT_9_SEQUENCE=TTTCACAGTTCCGCTCAGATATT
PRIMER_LEFT_9=4559,23
PRIMER_RIGHT_9=4911,23
PRIMER_LEFT_9_TM=59.896
PRIMER_RIGHT_9_TM=60.143
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=5.8000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=353
PRIMER_PAIR_PENALTY_10=0.2468
PRIMER_LEFT_10_PENALTY=0.104238
PRIMER_RIGHT_10_PENALTY=0.142611
PRIMER_LEFT_10_SEQUENCE=TTCTCCAGTCCCTAGTCCTCTCT
PRIMER_RIGHT_10_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_10=4558,23
PRIMER_RIGHT_10=4909,23
PRIMER_LEFT_10_TM=59.896
PRIMER_RIGHT_10_TM=60.143
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=39.130
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=7.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=352
PRIMER_PAIR_PENALTY_11=0.2468
PRIMER_LEFT_11_PENALTY=0.104238
PRIMER_RIGHT_11_PENALTY=0.142611
PRIMER_LEFT_11_SEQUENCE=TTCTCCAGTCCCTAGTCCTCTCT
PRIMER_RIGHT_11_SEQUENCE=TTTCACAGTTCCGCTCAGATATT
PRIMER_LEFT_11=4558,23
PRIMER_RIGHT_11=4911,23
PRIMER_LEFT_11_TM=59.896
PRIMER_RIGHT_11_TM=60.143
PRIMER_LEFT_11_GC_PERCENT=52.174
PRIMER_RIGHT_11_GC_PERCENT=39.130
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MER21B a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=5.8000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=354
PRIMER_PAIR_PENALTY_12=0.2567
PRIMER_LEFT_12_PENALTY=0.225815
PRIMER_RIGHT_12_PENALTY=0.030877
PRIMER_LEFT_12_SEQUENCE=CCAGTCCCTAGTCCTCTCTTTTC
PRIMER_RIGHT_12_SEQUENCE=ACAGCAATAACTTAGGGGAGGAG
PRIMER_LEFT_12=4562,23
PRIMER_RIGHT_12=4716,23
PRIMER_LEFT_12_TM=59.774
PRIMER_RIGHT_12_TM=60.031
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, SVA Composite retroposon
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=7.3000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=155
PRIMER_PAIR_PENALTY_13=0.2567
PRIMER_LEFT_13_PENALTY=0.225815
PRIMER_RIGHT_13_PENALTY=0.030877
PRIMER_LEFT_13_SEQUENCE=TCCAGTCCCTAGTCCTCTCTTTT
PRIMER_RIGHT_13_SEQUENCE=ACAGCAATAACTTAGGGGAGGAG
PRIMER_LEFT_13=4561,23
PRIMER_RIGHT_13=4716,23
PRIMER_LEFT_13_TM=59.774
PRIMER_RIGHT_13_TM=60.031
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=4.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=0.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=11.00, reverse MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=7.3000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_13=156
PRIMER_PAIR_PENALTY_14=0.2601
PRIMER_LEFT_14_PENALTY=0.091943
PRIMER_RIGHT_14_PENALTY=0.168205
PRIMER_LEFT_14_SEQUENCE=AAGCAGTTGGAGAGAGAAGGATT
PRIMER_RIGHT_14_SEQUENCE=TCATCCAGAAGACTCTCATCCTC
PRIMER_LEFT_14=3163,23
PRIMER_RIGHT_14=3430,23
PRIMER_LEFT_14_TM=59.908
PRIMER_RIGHT_14_TM=59.832
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_14_MISPRIMING_SCORE=21.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_14_END_STABILITY=8.1000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=268
PRIMER_PAIR_PENALTY_15=0.3459
PRIMER_LEFT_15_PENALTY=0.203244
PRIMER_RIGHT_15_PENALTY=0.142611
PRIMER_LEFT_15_SEQUENCE=CAGTGAAGTTTCTCCAGTCCCTA
PRIMER_RIGHT_15_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_15=4549,23
PRIMER_RIGHT_15=4909,23
PRIMER_LEFT_15_TM=59.797
PRIMER_RIGHT_15_TM=60.143
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=21.00, reverse MER42c MER42 repetitive sequence: subfamily MER42c - a consensus
PRIMER_LEFT_15_END_STABILITY=8.7000
PRIMER_RIGHT_15_END_STABILITY=7.2000
PRIMER_PAIR_15_COMPL_ANY=4.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=361
PRIMER_PAIR_PENALTY_16=0.3459
PRIMER_LEFT_16_PENALTY=0.203244
PRIMER_RIGHT_16_PENALTY=0.142611
PRIMER_LEFT_16_SEQUENCE=CAGTGAAGTTTCTCCAGTCCCTA
PRIMER_RIGHT_16_SEQUENCE=TTTCACAGTTCCGCTCAGATATT
PRIMER_LEFT_16=4549,23
PRIMER_RIGHT_16=4911,23
PRIMER_LEFT_16_TM=59.797
PRIMER_RIGHT_16_TM=60.143
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=8.7000
PRIMER_RIGHT_16_END_STABILITY=5.8000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=363
PRIMER_PAIR_PENALTY_17=0.3586
PRIMER_LEFT_17_PENALTY=0.088543
PRIMER_RIGHT_17_PENALTY=0.270039
PRIMER_LEFT_17_SEQUENCE=ACATGATCCATAGGGATGTGAAG
PRIMER_RIGHT_17_SEQUENCE=AATGTGGTATAAGGCACTCATCG
PRIMER_LEFT_17=1219,23
PRIMER_RIGHT_17=1493,23
PRIMER_LEFT_17_TM=60.089
PRIMER_RIGHT_17_TM=60.270
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=8.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=8.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=275
PRIMER_PAIR_PENALTY_18=0.3667
PRIMER_LEFT_18_PENALTY=0.150712
PRIMER_RIGHT_18_PENALTY=0.215994
PRIMER_LEFT_18_SEQUENCE=TCTAAACCTGGGTCTCTGTTTCA
PRIMER_RIGHT_18_SEQUENCE=AATATCTGAACGGGATGGAGACT
PRIMER_LEFT_18=356,23
PRIMER_RIGHT_18=482,23
PRIMER_LEFT_18_TM=60.151
PRIMER_RIGHT_18_TM=60.216
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=11.00, MER4C a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MER4C a consensus
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=6.1000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=127
PRIMER_PAIR_PENALTY_19=0.3684
PRIMER_LEFT_19_PENALTY=0.225815
PRIMER_RIGHT_19_PENALTY=0.142611
PRIMER_LEFT_19_SEQUENCE=CCAGTCCCTAGTCCTCTCTTTTC
PRIMER_RIGHT_19_SEQUENCE=TCACAGTTCCGCTCAGATATTTT
PRIMER_LEFT_19=4562,23
PRIMER_RIGHT_19=4909,23
PRIMER_LEFT_19_TM=59.774
PRIMER_RIGHT_19_TM=60.143
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, SVA Composite retroposon
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.3000
PRIMER_RIGHT_19_END_STABILITY=7.2000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=348
=