PRIMER_SEQUENCE_ID=NM_017433
SEQUENCE=AGGGAGCGCCCGTAGCCAGCGCCCCCGTAAAGAAATAAGGAGGCGCCCGGCTTCCCGCCCAGGCCCAGGGCCCCAGTGCAGCCTGGACACAGTATTCAAAGTTTTGGCGTCTGAGCATTGTTATGCGTTATTTTCAAGCTTTGCTAACGGTTTTTAAACCTTTGAGATGTTTCCATTAATTGGAAAAACAATCATCTTTGATAACTTTCCTGATCCTTCTGATACATGGGAAATCACTGAGACAATTGGCAAAGGAACTTATGGGAAAGTTTTTAAAGTATTGAATAAGAAAAATGGCCAAAAAGCAGCAGTCAAAATTCTTGATCCAATTCACGATATTGACGAAGAGATTGAAGCAGAATATAACATCTTAAAAGCACTTTCTGACCACCCTAATGTGGTCAGATTCTATGGGATATACTTTAAGAAGGATAAAGTAAATGGAGACAAGCTGTGGTTGGTTCTTGAGCTCTGCAGTGGAGGATCAGTGACTGACCTTGTGAAAGGATTTCTGAAGAGGGGTGAAAGAATGAGTGAGCCTCTAATTGCCTATATTTTACATGAAGCACTAATGGGACTTCAACATTTGCATAACAACAAAACTATCCACAGAGATGTGAAAGGCAATAACATTCTATTGACCACGGAAGGTGGAGTGAAACTAGTAGATTTTGGTGTGTCTGCACAGCTCACCAGTACCCGGCACCGTCGGAACACATCCGTAGGAACACCGTTTTGGATGGCTCCTGAGGTGATTGCATGTGAACAGCAATTGGATACCACTTATGACGCCAGATGTGACACTTGGTCCCTGGGTATCACGGCCATTGAGCTGGGTGATGGAGATCCTCCACTAGCTGACCTTCATCCCATGAGAGCACTCTTCAAAATACCAAGGAATCCACCCCCAAAACTAAGGCAGCCTGAGCTATGGTCAGCAGAATTCAATGACTTCATAAGCAAGTGCTTGACTAAAGATTATGAAAAGCGTCCAACAGTGTCAGAACTTTTACAGCATAAATTCATTACTCAAATTGAGGGCAAAGATGTGATGCTACAAAAACAACTAACGGAATTCATTGGCATCCATCAATGCATGGGAGGCACAGAAAAGGCCAGACGTGAACGTATTCACACGAAGAAAGGGAACTTCAACCGACCTCTAATATCCAATCTGAAGGATGTAGATGATTTAGCAACCCTAGAAATTTTGGATGAGAATACAGTCTCAGAGCAACTTGAGAAGTGTTATTCCAGAGATCAGATCTACGTCTATGTGGGAGACATACTCATTGCTCTTAACCCTTTTCAGAGTCTGGGTCTTTACTCCACAAAGCATTCCAAACTATATATTGGATCAAAGAGAACTGCCAGTCCTCCTCACATTTTTGCAATGGCTGACTTAGGATATCAATCTATAATAACATATAATTCAGATCAGTGCATTGTTATTTCTGGAGAAAGTGGTGCTGGAAAGACTGAAAATGCTCATCTTTTAGTTCAGCAGCTGACAGTGCTTGGAAAGGCTAATAACAGAACCTTGCAAGAGAAGATTTTACAAGTGAACAATTTGGTAGAAGCCTTTGGCAATGCCTGCACTATTATAAATGACAATTCTAGCAGATTTGGAAAATACTTAGAAATGAAATTCACCTCTTCTGGAGCGGTAGTGGGAGCACAGATTTCTGAATATCTCCTGGAAAAATCCCGAGTTATCCACCAAGCTATTGGAGAAAAAAATTTTCATATTTTTTACTACATTTATGCTGGTTTGGCTGAAAAGAAGAAACTAGCCCATTACAAACTGCCTGAAAATAAGCCTCCCAGGTACCTACAAAATGACCACCTCAGAACAGTACAAGACATCATGAATAATAGTTTCTATAAATCCCAGTATGAATTAATTGAGCAATGTTTCAAAGTCATAGGTTTTACAATGGAGCAACTTGGTAGTATATACAGCATACTCGCTGCAATCTTGAATGTTGGCAACATTGAATTTTCTTCTGTGGCAACTGAACACCAGATTGACAAGAGCCACATTTCTAATCATACAGCCCTGGAGAACTGTGTTTCTTTGCTTTGCATTCGGGCAGATGAGCTACAAGAAGCTCTCACCTCCCACTGTGTGGTCACTAGAGGAGAAACAATTATACGACCCAATACTGTAGAAAAAGCTACCGATGTCAGGGATGCCATGGCTAAAACTTTATATGGACGTCTCTTTAGTTGGATAGTCAATTGCATTAACAGTTTGTTGAAGCATGACTCATCACCAAGTGGGAATGGTGATGAGCTGAGCATTGGCATTCTTGATATATTTGGTTTTGAAAATTTCAAAAAAAATTCCTTCGAGCAGCTGTGCATTAACATTGCAAATGAACAAATTCAGTATTATTATAATCAACATGTGTTTGCATGGGAACAGAATGAATACCTAAATGAAGATGTGGATGCTAGAGTTATTGAATATGAGGATAACTGGCCCCTCTTAGATATGTTTCTGCAAAAGCCAATGGGTTTACTTTCCCTACTTGATGAAGAAAGTAGATTTCCCAAGGCCACTGACCAGACTCTTGTAGAAAAATTTGAAGGTAACCTGAAATCACAATACTTCTGGAGACCCAAAAGAATGGAACTTAGTTTTGGAATTCACCATTATGCAGGAAAGGTCCTCTATAATGCAAGTGGATTCTTAGCCAAAAACAGAGACACTCTTCCTACTGACATTGTGCTACTTTTGAGGTCATCCGACAACAGTGTAATTAGGCAACTAGTCAACCACCCTCTGACCAAAACAGGTAATCTGCCACATTCTAAAACTAAAAATGTTATAAACTATCAAATGAGGACTTCAGAAAAATTAATCAACCTGGCAAAGGGCGACACTGGAGAAGCCACACGTCATGCCAGAGAGACAACCAACATGAAAACACAAACGGTTGCATCATATTTTAGATATTCCCTGATGGATTTGTTGTCTAAAATGGTGGTGGGCCAACCTCATTTTGTCCGTTGCATCAAACCAAATAGTGAGCGTCAGGCAAGAAAATATGACAAAGAGAAAGTTCTGCTACAGCTTCGGTACACAGGAATTCTGGAAACAGCAAGAATTCGAAGACTAGGATTCTCCCATCGGATACTTTTTGCTAACTTTATAAAGCGGTACTACCTTCTCTGCTACAAGTCGAGCGAGGAGCCCCGCATGAGCCCTGACACCTGTGCCACCATTTTGGAAAAAGCTGGTCTCGATAACTGGGCTCTTGGAAAAACAAAAGTGTTCCTTAAGTATTATCACGTGGAGCAGTTAAATCTAATGCGAAAGGAAGCTATTGACAAGCTTATTTTGATTCAAGCTTGTGTCAGAGCATTCTTGTGTTCAAGAAGATACCAAAAAATACAGGAGAAAAGGAAAGAAAGCGCTATAATAATACAGTCAGCTGCAAGAGGACACCTTGTCGGGAAACAAAGAAAAGAAATTGTTGACATGAAAAACACAGCAGTAACAACCATTCAAACTTCTGATCAGGAATTCGACTACAAGAAAAACTTTGAAAATACAAGGGAATCTTTCGTGAAGAAACAAGCAGAAAATGCAATCTCTGCTAATGAAAGATTCATTTCAGCTCCAAATAATAAAGGAAGTGTATCTGTAGTGAAGACTTCCACTTTCAAACCTGAAGAGGAAACCACCAATGCTGTGGAGAGTAACAACAGAGTGTATCAGACTCCAAAAAAAATGAATAATGTGTATGAGGAAGAGGTTAAGCAAGAATTTTACCTTGTAGGGCCAGAAGTAAGCCCCAAACAGAAGTTTGTCAAAGACCTGGAAGAGAACAGCAATCTAAGGAAAGTGGAGAAAGAGGAAGCTATGATCCAGAGTTACTATCAGAGGTACACAGAGGAGAGGAATTGTGAAGAGTCAAAAGCAGCATATCTAGAAAGGAAGGCCATATCAGAAAGGCCAAGCTACCCAGTGCCTTGGTTAGCTGAAAATGAGACTTCCTTTAAAAAAACTTTGGAACCTACACTTAGCCAAAGGTCAATTTATCAAAATGCAAACAGCATGGAAAAAGAAAAGAAGACATCTGTAGTTACCCAGAGTGCACCGATATGCAGCCAGGAGGAAGGCAGAGGCCGTCTGAGGCATGAGACAGTCAAAGAGAGGCAAGTTGAACCAGTGACACAGGCCCAGGAGGAAGAAGATAAAGCAGCGGTATTCATTCAGAGCAAATACCGGGGTTACAAGAGAAGGCAGCAGTTGAGGAAGGACAAGATGTCTTCTTTTAAGCATCAGAAGATTGTCACAACACCAACAGAAGTAGCAAGAAACACTCATAATTTGTATTCCTATCCCACAAAACATGAGGAAATCAATAACATCAAGAAGAAGGATAACAAAGACTCGAAAGCAACTTCAGAAAGAGAAGCATGTGGTTTGGCAATTTTTTCAAAACAGATATCAAAGTTATCTGAAGAATATTTCATTCTGCAGAAAAAATTGAATGAAATGATTTTGTCACAGCAACTGAAGTCACTTTATCTGGGTGTCTCGCACCATAAGCCAATTAATAGACGAGTTTCTTCTCAGCAGTGCCTCTCAGGTGTCTGTAAAGGAGAGGAGCCAAAAATATTGAGACCCCCAAGACGACCCCGGAAACCCAAAACATTAAATAACCCTGAAGACTCCACATACTATTATCTACTTCATAAGTCAATCCAAGAAGAAAAACGAAGACCAAGGAAAGACAGTCAGGGAAAATTATTAGATTTGGAAGATTTCTATTATAAGGAATTTTTGCCCAGTCGTTCTGGACCAAAGGAACATAGCCCTAGTTTAAGAGAACGAAGACCACAGCAAGAACTCCAGAATCAATGTATTAAGGCTAATGAAAGGTGCTGGGCGGCGGAGAGCCCCGAGAAGGAGGAGGAGAGAGAGCCAGCAGCCAACCCCTACGACTTCAGGAGGCTCCTGCGCAAAACCTCCCAGCGCCGGCGCCTCGTCCAGCAGTCCTAACCGTTCAACGAGGCAGTCACCGCCGTCGGAAGGCGCTGGAGCCTGCGGGGCAGCAGGGGCCAAGCAGGCACTCTGGGGCTGGCACCAGCAGGCACTGAAGCTGCGGCCCTGATCTCCGCAGAGGCTGCCTGCTGCGCTCGGCCCTCAAGTGCCCGGGCCGGCCTTCGTGCTCCGAAACAAGAGACCTGGGAGCCCTCGGGAAACCTCCCCCGACGCTCTCTCTCGGAACTCCCGCACCCTCCTTTCTCACCAGCCCGCCAGTTGTGGCAACCCTGTCCTTGTTCCCCTAATCTATCACTTTGTTCTTTTTTTTTGTGACTCCTGTGGACTCCACTGCGCCTGGGATCTCGCCAACCCCTCTCTCATTTGGGGTGACTGAATTCACAGATTTTTTTTTTTATTGGAAACGGCTTTTCTTGGCCAACAGAACACTTGCTAGCGGTTGAATCTTAGAGAAAAAAGCCCGGGAGGGGTGGGGAGAATTTCGAAGATGTATTTCATCTCAAGCTTGCTCTTTCTCTTCCTTTGGTTATTAAGGTCACTAAATAAAGGAAGTGCCTTGGAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 64457, GC content failed 2306, low tm 33824, high tm 13443, high any compl 8, high end compl 45, high repeat similarity 64, long poly-x seq 209, ok 14558
PRIMER_RIGHT_EXPLAIN=considered 64318, GC content failed 2011, low tm 34316, high tm 12994, high any compl 3, high end compl 14, high repeat similarity 15, long poly-x seq 231, ok 14734
PRIMER_PAIR_EXPLAIN=considered 1150, unacceptable product size 1077, high any compl 1, high end compl 5, ok 67
PRIMER_PAIR_PENALTY=0.0157
PRIMER_LEFT_PENALTY=0.013634
PRIMER_RIGHT_PENALTY=0.002035
PRIMER_LEFT_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_SEQUENCE=GTTTCTTCACGAAAGATTCCCTT
PRIMER_LEFT=3159,23
PRIMER_RIGHT=3583,23
PRIMER_LEFT_TM=59.986
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=39.130
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=425
PRIMER_PAIR_PENALTY_1=0.0232
PRIMER_LEFT_1_PENALTY=0.013634
PRIMER_RIGHT_1_PENALTY=0.009608
PRIMER_LEFT_1_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_1_SEQUENCE=TCCTGATCAGAAGTTTGAATGGT
PRIMER_LEFT_1=3159,23
PRIMER_RIGHT_1=3530,23
PRIMER_LEFT_1_TM=59.986
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=39.130
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=11.00, LTR9 LTR from human endogenous retrovirus-like sequence (HUERS-P3)
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=372
PRIMER_PAIR_PENALTY_2=0.0503
PRIMER_LEFT_2_PENALTY=0.013634
PRIMER_RIGHT_2_PENALTY=0.036623
PRIMER_LEFT_2_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_2_SEQUENCE=GTCCTCTTGCAGCTGACTGTATT
PRIMER_LEFT_2=3159,23
PRIMER_RIGHT_2=3451,23
PRIMER_LEFT_2_TM=59.986
PRIMER_RIGHT_2_TM=59.963
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT2A1 Interspersed repeat MLT2A1- a consensus
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.0571
PRIMER_LEFT_3_PENALTY=0.013634
PRIMER_RIGHT_3_PENALTY=0.043423
PRIMER_LEFT_3_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_3_SEQUENCE=TTTGGCTAAGTGTAGGTTCCAAA
PRIMER_LEFT_3=3159,23
PRIMER_RIGHT_3=4039,23
PRIMER_LEFT_3_TM=59.986
PRIMER_RIGHT_3_TM=60.043
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=39.130
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=10.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=881
PRIMER_PAIR_PENALTY_4=0.0628
PRIMER_LEFT_4_PENALTY=0.031944
PRIMER_RIGHT_4_PENALTY=0.030877
PRIMER_LEFT_4_SEQUENCE=TGAAATGATTTTGTCACAGCAAC
PRIMER_RIGHT_4_SEQUENCE=GTCTTTCCTTGGTCTTCGTTTTT
PRIMER_LEFT_4=4506,23
PRIMER_RIGHT_4=4749,23
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=34.783
PRIMER_RIGHT_4_GC_PERCENT=39.130
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=244
PRIMER_PAIR_PENALTY_5=0.0639
PRIMER_LEFT_5_PENALTY=0.013634
PRIMER_RIGHT_5_PENALTY=0.050278
PRIMER_LEFT_5_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_5_SEQUENCE=AACTTCTGTTTGGGGCTTACTTC
PRIMER_LEFT_5=3159,23
PRIMER_RIGHT_5=3815,23
PRIMER_LEFT_5_TM=59.986
PRIMER_RIGHT_5_TM=60.050
PRIMER_LEFT_5_GC_PERCENT=47.826
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=8.2000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=657
PRIMER_PAIR_PENALTY_6=0.0651
PRIMER_LEFT_6_PENALTY=0.016658
PRIMER_RIGHT_6_PENALTY=0.048411
PRIMER_LEFT_6_SEQUENCE=AAGATTATGAAAAGCGTCCAACA
PRIMER_RIGHT_6_SEQUENCE=CCTTTCTTCGTGTGAATACGTTC
PRIMER_LEFT_6=974,23
PRIMER_RIGHT_6=1145,23
PRIMER_LEFT_6_TM=60.017
PRIMER_RIGHT_6_TM=60.048
PRIMER_LEFT_6_GC_PERCENT=34.783
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=8.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=172
PRIMER_PAIR_PENALTY_7=0.0657
PRIMER_LEFT_7_PENALTY=0.059210
PRIMER_RIGHT_7_PENALTY=0.006468
PRIMER_LEFT_7_SEQUENCE=ATCTTTCGTGAAGAAACAAGCAG
PRIMER_RIGHT_7_SEQUENCE=AGTGACTTCAGTTGCTGTGACAA
PRIMER_LEFT_7=3567,23
PRIMER_RIGHT_7=4538,23
PRIMER_LEFT_7_TM=59.941
PRIMER_RIGHT_7_TM=60.006
PRIMER_LEFT_7_GC_PERCENT=39.130
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=7.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_7_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=8.2000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=972
PRIMER_PAIR_PENALTY_8=0.0663
PRIMER_LEFT_8_PENALTY=0.013634
PRIMER_RIGHT_8_PENALTY=0.052685
PRIMER_LEFT_8_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_8_SEQUENCE=ACCTTTGGCTAAGTGTAGGTTCC
PRIMER_LEFT_8=3159,23
PRIMER_RIGHT_8=4042,23
PRIMER_LEFT_8_TM=59.986
PRIMER_RIGHT_8_TM=59.947
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=7.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=11.00, MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=884
PRIMER_PAIR_PENALTY_9=0.0668
PRIMER_LEFT_9_PENALTY=0.048411
PRIMER_RIGHT_9_PENALTY=0.018372
PRIMER_LEFT_9_SEQUENCE=GAACGTATTCACACGAAGAAAGG
PRIMER_RIGHT_9_SEQUENCE=GCAAAGCAAAGAAACACAGTTCT
PRIMER_LEFT_9=1123,23
PRIMER_RIGHT_9=2085,23
PRIMER_LEFT_9_TM=60.048
PRIMER_RIGHT_9_TM=59.982
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=8.5000
PRIMER_RIGHT_9_END_STABILITY=6.4000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=963
PRIMER_PAIR_PENALTY_10=0.0707
PRIMER_LEFT_10_PENALTY=0.064245
PRIMER_RIGHT_10_PENALTY=0.006468
PRIMER_LEFT_10_SEQUENCE=CTTCAGAAAGAGAAGCATGTGGT
PRIMER_RIGHT_10_SEQUENCE=AGTGACTTCAGTTGCTGTGACAA
PRIMER_LEFT_10=4415,23
PRIMER_RIGHT_10=4538,23
PRIMER_LEFT_10_TM=59.936
PRIMER_RIGHT_10_TM=60.006
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=3.00
PRIMER_LEFT_10_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.6000
PRIMER_RIGHT_10_END_STABILITY=6.7000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_10=124
PRIMER_PAIR_PENALTY_11=0.0740
PRIMER_LEFT_11_PENALTY=0.067516
PRIMER_RIGHT_11_PENALTY=0.006468
PRIMER_LEFT_11_SEQUENCE=TGTGAAGAGTCAAAAGCAGCATA
PRIMER_RIGHT_11_SEQUENCE=AGTGACTTCAGTTGCTGTGACAA
PRIMER_LEFT_11=3913,23
PRIMER_RIGHT_11=4538,23
PRIMER_LEFT_11_TM=60.068
PRIMER_RIGHT_11_TM=60.006
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=3.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.4000
PRIMER_RIGHT_11_END_STABILITY=6.7000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=626
PRIMER_PAIR_PENALTY_12=0.0769
PRIMER_LEFT_12_PENALTY=0.048411
PRIMER_RIGHT_12_PENALTY=0.028502
PRIMER_LEFT_12_SEQUENCE=GAACGTATTCACACGAAGAAAGG
PRIMER_RIGHT_12_SEQUENCE=AGCAAAGAAACACAGTTCTCCAG
PRIMER_LEFT_12=1123,23
PRIMER_RIGHT_12=2081,23
PRIMER_LEFT_12_TM=60.048
PRIMER_RIGHT_12_TM=59.971
PRIMER_LEFT_12_GC_PERCENT=43.478
PRIMER_RIGHT_12_GC_PERCENT=43.478
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=8.5000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=959
PRIMER_PAIR_PENALTY_13=0.0817
PRIMER_LEFT_13_PENALTY=0.031944
PRIMER_RIGHT_13_PENALTY=0.049761
PRIMER_LEFT_13_SEQUENCE=TGAAATGATTTTGTCACAGCAAC
PRIMER_RIGHT_13_SEQUENCE=CTCTAAGATTCAACCGCTAGCAA
PRIMER_LEFT_13=4506,23
PRIMER_RIGHT_13=5471,23
PRIMER_LEFT_13_TM=60.032
PRIMER_RIGHT_13_TM=60.050
PRIMER_LEFT_13_GC_PERCENT=34.783
PRIMER_RIGHT_13_GC_PERCENT=43.478
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_13_END_STABILITY=8.2000
PRIMER_RIGHT_13_END_STABILITY=8.5000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_13=966
PRIMER_PAIR_PENALTY_14=0.0951
PRIMER_LEFT_14_PENALTY=0.064245
PRIMER_RIGHT_14_PENALTY=0.030877
PRIMER_LEFT_14_SEQUENCE=CTTCAGAAAGAGAAGCATGTGGT
PRIMER_RIGHT_14_SEQUENCE=GTCTTTCCTTGGTCTTCGTTTTT
PRIMER_LEFT_14=4415,23
PRIMER_RIGHT_14=4749,23
PRIMER_LEFT_14_TM=59.936
PRIMER_RIGHT_14_TM=60.031
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=39.130
PRIMER_LEFT_14_SELF_ANY=5.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=12.00, MER25 Older LINE1 5' end region - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_14_MISPRIMING_SCORE=23.00, reverse MER4C a consensus
PRIMER_LEFT_14_END_STABILITY=7.6000
PRIMER_RIGHT_14_END_STABILITY=7.6000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=335
PRIMER_PAIR_PENALTY_15=0.0952
PRIMER_LEFT_15_PENALTY=0.013634
PRIMER_RIGHT_15_PENALTY=0.081604
PRIMER_LEFT_15_SEQUENCE=AAAGCGGTACTACCTTCTCTGCT
PRIMER_RIGHT_15_SEQUENCE=TTTGAATGGTTGTTACTGCTGTG
PRIMER_LEFT_15=3159,23
PRIMER_RIGHT_15=3517,23
PRIMER_LEFT_15_TM=59.986
PRIMER_RIGHT_15_TM=60.082
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=39.130
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=4.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_15_MISPRIMING_SCORE=20.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=8.2000
PRIMER_RIGHT_15_END_STABILITY=6.7000
PRIMER_PAIR_15_COMPL_ANY=7.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=359
PRIMER_PAIR_PENALTY_16=0.0984
PRIMER_LEFT_16_PENALTY=0.067516
PRIMER_RIGHT_16_PENALTY=0.030877
PRIMER_LEFT_16_SEQUENCE=TGTGAAGAGTCAAAAGCAGCATA
PRIMER_RIGHT_16_SEQUENCE=GTCTTTCCTTGGTCTTCGTTTTT
PRIMER_LEFT_16=3913,23
PRIMER_RIGHT_16=4749,23
PRIMER_LEFT_16_TM=60.068
PRIMER_RIGHT_16_TM=60.031
PRIMER_LEFT_16_GC_PERCENT=39.130
PRIMER_RIGHT_16_GC_PERCENT=39.130
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_16_END_STABILITY=7.4000
PRIMER_RIGHT_16_END_STABILITY=7.6000
PRIMER_PAIR_16_COMPL_ANY=5.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=837
PRIMER_PAIR_PENALTY_17=0.1022
PRIMER_LEFT_17_PENALTY=0.016658
PRIMER_RIGHT_17_PENALTY=0.085505
PRIMER_LEFT_17_SEQUENCE=AAGATTATGAAAAGCGTCCAACA
PRIMER_RIGHT_17_SEQUENCE=TCTGAGGTGGTCATTTTGTAGGT
PRIMER_LEFT_17=974,23
PRIMER_RIGHT_17=1851,23
PRIMER_LEFT_17_TM=60.017
PRIMER_RIGHT_17_TM=59.914
PRIMER_LEFT_17_GC_PERCENT=34.783
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.0000
PRIMER_RIGHT_17_END_STABILITY=6.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=878
PRIMER_PAIR_PENALTY_18=0.1026
PRIMER_LEFT_18_PENALTY=0.059210
PRIMER_RIGHT_18_PENALTY=0.043423
PRIMER_LEFT_18_SEQUENCE=ATCTTTCGTGAAGAAACAAGCAG
PRIMER_RIGHT_18_SEQUENCE=TTTGGCTAAGTGTAGGTTCCAAA
PRIMER_LEFT_18=3567,23
PRIMER_RIGHT_18=4039,23
PRIMER_LEFT_18_TM=59.941
PRIMER_RIGHT_18_TM=60.043
PRIMER_LEFT_18_GC_PERCENT=39.130
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=10.00, THE1BR THE-1B Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=8.2000
PRIMER_RIGHT_18_END_STABILITY=8.8000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=473
PRIMER_PAIR_PENALTY_19=0.1039
PRIMER_LEFT_19_PENALTY=0.085505
PRIMER_RIGHT_19_PENALTY=0.018372
PRIMER_LEFT_19_SEQUENCE=ACCTACAAAATGACCACCTCAGA
PRIMER_RIGHT_19_SEQUENCE=GCAAAGCAAAGAAACACAGTTCT
PRIMER_LEFT_19=1829,23
PRIMER_RIGHT_19=2085,23
PRIMER_LEFT_19_TM=59.914
PRIMER_RIGHT_19_TM=59.982
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=39.130
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1f (MLT1f subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.4000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=257
=