PRIMER_SEQUENCE_ID=NM_020168
SEQUENCE=GTCCCCCTGCTGGGGACAGCAGCTGGGTCCAGCAGAGAGCTGGCTGGGGGCTTGACAGGAGTTTATCCTAACTGATGCTGGGTGGGCGCAGGCCCTGAGATGGGCGTCAGGGAGAGGGGACGCCAGACACACGGCCTGAGCAGGGTGAGGAGCAGCTGCCAGCCCCGGCAGGCCCAGGCCCTGTCTGGGTGCACAGGACAGAGACACAGCAAGATGCCCAGGGAGTGCCGCTTCCTGGGCTAGAGACAAGCACCAGCCTGCAGTGGAGAACGCAGGACCCCGCTGCCCAGAAGGAGCAGCCACGGCCTGCGGAGGACTGGCCCAGCAAGGTCCCAGGTCTTCCCTCTCCTCAGCGCCTAAGAGAGAGGCCCAGTGCGGGTGAGGAGTCGCGAGGAAGAGGCGGAAGGCGCCGGAAGGACCATGTTCCGCAAGAAAAAGAAGAAACGCCCTGAGATCTCAGCGCCACAGAACTTCCAGCACCGTGTCCACACCTCCTTCGACCCCAAAGAAGGCAAGTTTGTGGGCCTCCCCCCACAATGGCAGAACATCCTGGACACACTGCGGCGCCCCAAGCCCGTGGTGGACCCTTCGCGAATCACACGGGTGCAGCTCCAGCCCATGAAGACAGTGGTGCGGGGCAGCGCGATGCCTGTGGATGGCTACATCTCGGGGCTGCTCAACGACATCCAGAAGTTGTCAGTCATCAGCTCCAACACCCTGCGTGGCCGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCTGGGGGATGAGCACTGGGCCACCGACCCAGACATGTACCTCCAGAGCCCCCAGTCTGAGCGCACTGACCCCCACGGCCTCTACCTCAGCTGCAACGGGGGCACACCAGCAGGCCACAAGCAGATGCCGTGGCCCGAGCCACAGAGCCCACGGGTCCTGCCCAATGGGCTGGCTGCAAAGGCACAGTCCCTGGGCCCCGCCGAGTTTCAGGGTGCCTCGCAGCGCTGTCTGCAGCTGGGTGCCTGCCTGCAGAGCTCCCCACCAGGAGCCTCGCCCCCCACGGGCACCAATAGGCATGGAATGAAGGCTGCCAAGCATGGCTCTGAGGAGGCCCGGCCACAGTCCTGCCTGGTGGGCTCAGCCACAGGCAGGCCAGGTGGGGAAGGCAGCCCTAGCCCTAAGACCCGGGAGAGCAGCCTGAAGCGCAGGCTATTCCGAAGCATGTTCCTGTCCACTGCTGCCACAGCCCCTCCAAGCAGCAGCAAGCCAGGCCCTCCACCACAGAGCAAGCCCAACTCCTCTTTCCGACCGCCGCAGAAAGACAACCCCCCAAGCCTGGTGGCCAAGGCCCAGTCCTTGCCCTCGGACCAGCCGGTGGGGACCTTCAGCCCTCTGACCACTTCGGATACCAGCAGCCCCCAGAAGTCCCTCCGCACAGCCCCGGCCACAGGCCAGCTTCCAGGCCGGTCTTCCCCAGCGGGATCCCCCCGCACCTGGCACGCCCAGATCAGCACCAGCAACCTGTACCTGCCCCAGGACCCCACGGTTGCCAAGGGTGCCCTGGCTGGTGAGGACACAGGTGTTGTGACACATGAGCAGTTCAAGGCTGCGCTCAGGATGGTGGTGGACCAGGGTGACCCCCGGCTGCTGCTGGACAGCTACGTGAAGATTGGCGAGGGCTCCACCGGCATCGTCTGCTTGGCCCGGGAGAAGCACTCGGGCCGCCAGGTGGCCGTCAAGATGATGGACCTCAGGAAGCAGCAGCGCAGGGAGCTGCTCTTCAACGAGGTGGTGATCATGCGGGACTACCAGCACTTCAACGTGGTGGAGATGTACAAGAGCTACCTGGTGGGCGAGGAGCTGTGGGTGCTCATGGAGTTCCTGCAGGGAGGAGCCCTCACAGACATCGTCTCCCAAGTCAGGCTGAATGAGGAGCAGATTGCCACTGTGTGTGAGGCTGTGCTGCAGGCCCTGGCCTACCTGCATGCTCAGGGTGTCATCCACCGGGACATCAAGAGTGACTCCATCCTGCTGACCCTCGATGGCAGGGTGAAGCTCTCGGACTTCGGATTCTGTGCTCAGATCAGCAAAGACGTCCCTAAGAGGAAGTCCCTGGTGGGAACCCCCTACTGGATGGCTCCTGAAGTGATCTCCAGGTCTTTGTATGCCACTGAGGTGGATATCTGGTCTCTGGGCATCATGGTGATTGAGATGGTAGATGGGGAGCCACCGTACTTCAGTGACTCCCCAGTGCAAGCCATGAAGAGGCTCCGGGACAGCCCCCCACCCAAGCTGAAAAACTCTCACAAGGTCTCCCCAGTGCTGCGAGACTTCCTGGAGCGGATGCTGGTGCGGGACCCCCAAGAGAGAGCCACAGCCCAGGAGCTCCTAGACCACCCCTTCCTGCTGCAGACAGGGCTACCTGAGTGCCTGGTGCCCCTGATCCAGCTCTACCGAAAGCAGACCTCCACCTGCTGAGCCCACCCCAAGTATGCCTGCCACCTACGCCCACAGGCAGGGCACACTGGGCAGCCAGCCTGCCGGCAGGACTTGCCTGCCTCCTCCTCTCAGTATTCTCTCCAAAGATTGAAATGTGAAGCCCCAGCCCCACCCTCTGCCCTTCAGCCTACTGGGCCAGGCCGGACCTGCCCCCTCAGTGTCTCTCCCTCCCGAGTCCCCAGATGGAGACCCCTTTCTACAGGATGACCCCTTGATATTTGCACAGGGATATTTCTAAGAAACGCAGAGGCCAGCGTTCCTGGCCTCTGCAGCCAACACAGTAGAAAAGGCTGCTGTGGTTTTTTAAAGGCAGTTGTCCACTAGTGTCCTAGGCCACTGCAGAGGGCAGACTGCTGGTCTCCACAGATACCTGCTGTTCTCAGCTCCAGCTTCAAACCTCGAGTCTCGAGAGGGCCACGGGGTGGTTTTTATGACCGGAATCCCGCTTCCTCCCTCACGTCTGATGTCCTGAAGGTGCAGTCCCACCTGTACAGCCCCTCCCCGCCCAGAACTGTGAATGGCCTGCTCCAGGCCATGGCTGGGGGCAGGGAGTGAGGGGACAATTTCTGAGTGAAAGAGAAAGAATGGGGTCGGTGGTGAAGGTGCTCTCACTTTACAGAATGGAGAGAACATCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGGAGGAAAGCCACCTTGACAGCCCAGGTCCCTCCAGGTCACCCACAGCCAGTTTCAGGAAGGCTGCCCCTCTCTCCCACTAAGTTCTGGCCTGAAGGGACCTGCTTTCTTGGCCTGGCTTCCACCTCTCCACTCCTGTGTCTACCTGGCCAGTGGAGTGGTCCATGCTAAGTCTAACACTCCTGGGAGCTCAGGAGGCTTCTGAGCTTCTCCTGTACTGTGCATCGTGAGGGCCAGAGACAGGAATGTAAGGATTGGCAACTGTGTTACCTTTCAAGTTTATCTCAATAACCAGGTCATCAGGGACCCATTGTTCTCTTCAGAACCCTATCTGGGAGAGAAGGCGAACCACCTCCGGGTTTCCATCATGTCAAGGTCACAGGCATCCATGTGTGCAAACCATCTGCCCCAGCTGCCTCCACAGACTGCTGTCTCCTTGTCCTCCTCGGCCCTGCCCCACTTCAGGGCTGCTGTGAGATGGAATTCCAGGAAAGAACTTCAGGTGTCTGGACCCTTTCTATCTAGATAATATTTTTAGATTCTTCTGCTCCCTAGTGACCTACCTGGGGGCAAAGAAATTGCAAGGACTTTTTTTTAAGGGTCAGAGTTTTCAAAACAAAAGCATCTTCCCTAGAAATTTTTGTGAATTGTTTGCACTTGTGCCTGTTTTAAATTAAATTGAGTGTTCAAAGCCCAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 44386, GC content failed 1076, low tm 10222, high tm 24584, high any compl 7, high end compl 26, high repeat similarity 34, long poly-x seq 149, ok 8288
PRIMER_RIGHT_EXPLAIN=considered 44261, GC content failed 1182, low tm 10439, high tm 24084, high any compl 2, high end compl 4, high repeat similarity 16, long poly-x seq 166, ok 8368
PRIMER_PAIR_EXPLAIN=considered 973, unacceptable product size 917, high end compl 6, ok 50
PRIMER_PAIR_PENALTY=0.0708
PRIMER_LEFT_PENALTY=0.018344
PRIMER_RIGHT_PENALTY=0.052460
PRIMER_LEFT_SEQUENCE=GTGATCTCCAGGTCTTTGTATGC
PRIMER_RIGHT_SEQUENCE=ACACGATGTTCTCTCCATTCTGT
PRIMER_LEFT=2133,23
PRIMER_RIGHT=3124,23
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=992
PRIMER_PAIR_PENALTY_1=0.0708
PRIMER_LEFT_1_PENALTY=0.018344
PRIMER_RIGHT_1_PENALTY=0.052460
PRIMER_LEFT_1_SEQUENCE=GTGATCTCCAGGTCTTTGTATGC
PRIMER_RIGHT_1_SEQUENCE=ACACACGATGTTCTCTCCATTCT
PRIMER_LEFT_1=2133,23
PRIMER_RIGHT_1=3126,23
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=994
PRIMER_PAIR_PENALTY_2=0.1968
PRIMER_LEFT_2_PENALTY=0.018344
PRIMER_RIGHT_2_PENALTY=0.178426
PRIMER_LEFT_2_SEQUENCE=GTGATCTCCAGGTCTTTGTATGC
PRIMER_RIGHT_2_SEQUENCE=CCATTCTGTAAAGTGAGAGCACC
PRIMER_LEFT_2=2133,23
PRIMER_RIGHT_2=3110,23
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.178
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=978
PRIMER_PAIR_PENALTY_3=0.2073
PRIMER_LEFT_3_PENALTY=0.018344
PRIMER_RIGHT_3_PENALTY=0.188921
PRIMER_LEFT_3_SEQUENCE=GTGATCTCCAGGTCTTTGTATGC
PRIMER_RIGHT_3_SEQUENCE=GAACAGCAGGTATCTGTGGAGAC
PRIMER_LEFT_3=2133,23
PRIMER_RIGHT_3=2865,23
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=60.189
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=733
PRIMER_PAIR_PENALTY_4=0.2126
PRIMER_LEFT_4_PENALTY=0.160134
PRIMER_RIGHT_4_PENALTY=0.052460
PRIMER_LEFT_4_SEQUENCE=TGAAGTGATCTCCAGGTCTTTGT
PRIMER_RIGHT_4_SEQUENCE=ACACACGATGTTCTCTCCATTCT
PRIMER_LEFT_4=2129,23
PRIMER_RIGHT_4=3126,23
PRIMER_LEFT_4_TM=60.160
PRIMER_RIGHT_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=998
PRIMER_PAIR_PENALTY_5=0.2126
PRIMER_LEFT_5_PENALTY=0.160134
PRIMER_RIGHT_5_PENALTY=0.052460
PRIMER_LEFT_5_SEQUENCE=TGAAGTGATCTCCAGGTCTTTGT
PRIMER_RIGHT_5_SEQUENCE=ACACGATGTTCTCTCCATTCTGT
PRIMER_LEFT_5=2129,23
PRIMER_RIGHT_5=3124,23
PRIMER_LEFT_5_TM=60.160
PRIMER_RIGHT_5_TM=60.052
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=6.4000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=996
PRIMER_PAIR_PENALTY_6=0.2313
PRIMER_LEFT_6_PENALTY=0.178873
PRIMER_RIGHT_6_PENALTY=0.052460
PRIMER_LEFT_6_SEQUENCE=AGACTGCTGGTCTCCACAGATAC
PRIMER_RIGHT_6_SEQUENCE=ACACACGATGTTCTCTCCATTCT
PRIMER_LEFT_6=2834,23
PRIMER_RIGHT_6=3126,23
PRIMER_LEFT_6_TM=59.821
PRIMER_RIGHT_6_TM=60.052
PRIMER_LEFT_6_GC_PERCENT=52.174
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, reverse MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.3000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=293
PRIMER_PAIR_PENALTY_7=0.2313
PRIMER_LEFT_7_PENALTY=0.178873
PRIMER_RIGHT_7_PENALTY=0.052460
PRIMER_LEFT_7_SEQUENCE=AGACTGCTGGTCTCCACAGATAC
PRIMER_RIGHT_7_SEQUENCE=ACACGATGTTCTCTCCATTCTGT
PRIMER_LEFT_7=2834,23
PRIMER_RIGHT_7=3124,23
PRIMER_LEFT_7_TM=59.821
PRIMER_RIGHT_7_TM=60.052
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=12.00, reverse MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.3000
PRIMER_RIGHT_7_END_STABILITY=6.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=291
PRIMER_PAIR_PENALTY_8=0.2347
PRIMER_LEFT_8_PENALTY=0.052460
PRIMER_RIGHT_8_PENALTY=0.182211
PRIMER_LEFT_8_SEQUENCE=AGAATGGAGAGAACATCGTGTGT
PRIMER_RIGHT_8_SEQUENCE=AACACAGTTGCCAATCCTTACAT
PRIMER_LEFT_8=3104,23
PRIMER_RIGHT_8=3441,23
PRIMER_LEFT_8_TM=60.052
PRIMER_RIGHT_8_TM=59.818
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=39.130
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=2.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER4C a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.4000
PRIMER_RIGHT_8_END_STABILITY=5.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=338
PRIMER_PAIR_PENALTY_9=0.2414
PRIMER_LEFT_9_PENALTY=0.188921
PRIMER_RIGHT_9_PENALTY=0.052460
PRIMER_LEFT_9_SEQUENCE=GTCTCCACAGATACCTGCTGTTC
PRIMER_RIGHT_9_SEQUENCE=ACACACGATGTTCTCTCCATTCT
PRIMER_LEFT_9=2843,23
PRIMER_RIGHT_9=3126,23
PRIMER_LEFT_9_TM=60.189
PRIMER_RIGHT_9_TM=60.052
PRIMER_LEFT_9_GC_PERCENT=52.174
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_9_MISPRIMING_SCORE=20.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=6.7000
PRIMER_RIGHT_9_END_STABILITY=6.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=284
PRIMER_PAIR_PENALTY_10=0.2414
PRIMER_LEFT_10_PENALTY=0.188921
PRIMER_RIGHT_10_PENALTY=0.052460
PRIMER_LEFT_10_SEQUENCE=GTCTCCACAGATACCTGCTGTTC
PRIMER_RIGHT_10_SEQUENCE=ACACGATGTTCTCTCCATTCTGT
PRIMER_LEFT_10=2843,23
PRIMER_RIGHT_10=3124,23
PRIMER_LEFT_10_TM=60.189
PRIMER_RIGHT_10_TM=60.052
PRIMER_LEFT_10_GC_PERCENT=52.174
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_10_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_10_END_STABILITY=6.7000
PRIMER_RIGHT_10_END_STABILITY=6.4000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_10=282
PRIMER_PAIR_PENALTY_11=0.2729
PRIMER_LEFT_11_PENALTY=0.052460
PRIMER_RIGHT_11_PENALTY=0.220453
PRIMER_LEFT_11_SEQUENCE=AGAATGGAGAGAACATCGTGTGT
PRIMER_RIGHT_11_SEQUENCE=GTAGACACAGGAGTGGAGAGGTG
PRIMER_LEFT_11=3104,23
PRIMER_RIGHT_11=3318,23
PRIMER_LEFT_11_TM=60.052
PRIMER_RIGHT_11_TM=60.220
PRIMER_LEFT_11_GC_PERCENT=43.478
PRIMER_RIGHT_11_GC_PERCENT=56.522
PRIMER_LEFT_11_SELF_ANY=4.00
PRIMER_RIGHT_11_SELF_ANY=3.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_11=215
PRIMER_PAIR_PENALTY_12=0.2843
PRIMER_LEFT_12_PENALTY=0.018344
PRIMER_RIGHT_12_PENALTY=0.265933
PRIMER_LEFT_12_SEQUENCE=GTGATCTCCAGGTCTTTGTATGC
PRIMER_RIGHT_12_SEQUENCE=GACACTAGTGGACAACTGCCTTT
PRIMER_LEFT_12=2133,23
PRIMER_RIGHT_12=2814,23
PRIMER_LEFT_12_TM=60.018
PRIMER_RIGHT_12_TM=59.734
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=8.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse Tigger2 Autonomous DNA transposon
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_12_END_STABILITY=7.4000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=682
PRIMER_PAIR_PENALTY_13=0.3136
PRIMER_LEFT_13_PENALTY=0.178873
PRIMER_RIGHT_13_PENALTY=0.134681
PRIMER_LEFT_13_SEQUENCE=AGACTGCTGGTCTCCACAGATAC
PRIMER_RIGHT_13_SEQUENCE=ACACACACACACGATGTTCTCTC
PRIMER_LEFT_13=2834,23
PRIMER_RIGHT_13=3132,23
PRIMER_LEFT_13_TM=59.821
PRIMER_RIGHT_13_TM=60.135
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MER41 Medium reiteration sequence MER41
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.3000
PRIMER_RIGHT_13_END_STABILITY=6.4000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=299
PRIMER_PAIR_PENALTY_14=0.3236
PRIMER_LEFT_14_PENALTY=0.188921
PRIMER_RIGHT_14_PENALTY=0.134681
PRIMER_LEFT_14_SEQUENCE=GTCTCCACAGATACCTGCTGTTC
PRIMER_RIGHT_14_SEQUENCE=ACACACACACACGATGTTCTCTC
PRIMER_LEFT_14=2843,23
PRIMER_RIGHT_14=3132,23
PRIMER_LEFT_14_TM=60.189
PRIMER_RIGHT_14_TM=60.135
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MER42a MER42 repetitive sequence: subfamily MER42a - a consensus
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=6.4000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=290
PRIMER_PAIR_PENALTY_15=0.3346
PRIMER_LEFT_15_PENALTY=0.018344
PRIMER_RIGHT_15_PENALTY=0.316302
PRIMER_LEFT_15_SEQUENCE=GTGATCTCCAGGTCTTTGTATGC
PRIMER_RIGHT_15_SEQUENCE=GAGAACAGCAGGTATCTGTGGAG
PRIMER_LEFT_15=2133,23
PRIMER_RIGHT_15=2867,23
PRIMER_LEFT_15_TM=60.018
PRIMER_RIGHT_15_TM=60.316
PRIMER_LEFT_15_GC_PERCENT=47.826
PRIMER_RIGHT_15_GC_PERCENT=52.174
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=735
PRIMER_PAIR_PENALTY_16=0.3386
PRIMER_LEFT_16_PENALTY=0.160134
PRIMER_RIGHT_16_PENALTY=0.178426
PRIMER_LEFT_16_SEQUENCE=TGAAGTGATCTCCAGGTCTTTGT
PRIMER_RIGHT_16_SEQUENCE=CCATTCTGTAAAGTGAGAGCACC
PRIMER_LEFT_16=2129,23
PRIMER_RIGHT_16=3110,23
PRIMER_LEFT_16_TM=60.160
PRIMER_RIGHT_16_TM=60.178
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=23.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_LEFT_16_END_STABILITY=7.0000
PRIMER_RIGHT_16_END_STABILITY=9.4000
PRIMER_PAIR_16_COMPL_ANY=6.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=982
PRIMER_PAIR_PENALTY_17=0.3400
PRIMER_LEFT_17_PENALTY=0.287569
PRIMER_RIGHT_17_PENALTY=0.052460
PRIMER_LEFT_17_SEQUENCE=CTGAAGTGATCTCCAGGTCTTTG
PRIMER_RIGHT_17_SEQUENCE=ACACACGATGTTCTCTCCATTCT
PRIMER_LEFT_17=2128,23
PRIMER_RIGHT_17=3126,23
PRIMER_LEFT_17_TM=60.288
PRIMER_RIGHT_17_TM=60.052
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=43.478
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, reverse L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, L1MD2 3'-end of L1 repeat (subfamily L1MD2) - a consensus sequence
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=6.6000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=999
PRIMER_PAIR_PENALTY_18=0.3400
PRIMER_LEFT_18_PENALTY=0.287569
PRIMER_RIGHT_18_PENALTY=0.052460
PRIMER_LEFT_18_SEQUENCE=CTGAAGTGATCTCCAGGTCTTTG
PRIMER_RIGHT_18_SEQUENCE=ACACGATGTTCTCTCCATTCTGT
PRIMER_LEFT_18=2128,23
PRIMER_RIGHT_18=3124,23
PRIMER_LEFT_18_TM=60.288
PRIMER_RIGHT_18_TM=60.052
PRIMER_LEFT_18_GC_PERCENT=47.826
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=1.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, reverse L1PA11 3'-end of L1 repeat (subfamily L1PA11) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_18_END_STABILITY=7.3000
PRIMER_RIGHT_18_END_STABILITY=6.4000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=997
PRIMER_PAIR_PENALTY_19=0.3491
PRIMER_LEFT_19_PENALTY=0.160134
PRIMER_RIGHT_19_PENALTY=0.188921
PRIMER_LEFT_19_SEQUENCE=TGAAGTGATCTCCAGGTCTTTGT
PRIMER_RIGHT_19_SEQUENCE=GAACAGCAGGTATCTGTGGAGAC
PRIMER_LEFT_19=2129,23
PRIMER_RIGHT_19=2865,23
PRIMER_LEFT_19_TM=60.160
PRIMER_RIGHT_19_TM=60.189
PRIMER_LEFT_19_GC_PERCENT=43.478
PRIMER_RIGHT_19_GC_PERCENT=52.174
PRIMER_LEFT_19_SELF_ANY=4.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=2.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, TAR1 Human telomere associated repeat sequence, complete sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=737
=