PRIMER_SEQUENCE_ID=NM_033507
SEQUENCE=CCTTTCAGTGCAGAAGCCCTGGGCTGCCAGCCTCAGGCAGCTCTCCATCCAAGCAGCCGTTGCTGCCACAGGCGGGCCTTACGCTCCAAGGCTACAGCATGTGCTAGGCCTCAGCAGGCAGGAGCATCTCTGCCTCCCAAAGCATCTACCTCTTAGCCCCTCGGAGAGATGGCGATGGATGTCACAAGGAGCCAGGCCCAGACAGCCTTGACTCTGGTAGAGCAGATCCTGGCAGAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGTGGCACTAACTTCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGCGTGAAGACCAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAGATGCTCTTCGACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAACACAAGAAGCTGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGATAAGGGCATCCTTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAATGTCGTGGGGCTTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTGGCAATGGTGAATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGCGAGGTCGGCATGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAATGTGGAGCTGGTGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTCGGGGACTCCGGCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAGAGCTCTGCAAACCCCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGCGAGCTGGTGCGGCTTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAGGCCTCCGAGCAGCTGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAGAGCGACACGGGCGACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCCTCGACCACCGACTGCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCGCACATGTGCTCGGCGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAGGACGTAATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTCAAGGAGCGGTTCCATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATCGAGTCGGAGGAGGGCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAGGCCTGTATGCTGGGCCAGTGAGAGCAGTGGCCGCAAGCGCAGGGAGGATGCCACAGCCCCACAGCACCCAGGCTCCATGGGGAAGTGCTCCCCACACGTGCTCGCAGCCTGGCGGGGCAGGAGGCCTGGCCTTGTCAGGACCCAGGCCGCCTGCCATACCGCTGGGGAACAGAGCGGGCCTCTTCCCTCAGTTTTTCGGTGGGACAGCCCCAGGGCCCTAACGGGGGTGCGGCAGGAGCAGGAACAGAGACTCTGGAAGCCCCCCACCTTTCTCGCTGGAATCAATTTCCCAGAAGGGAGTTGCTCACTCAGGACTTTGATGCATTTCCACACTGTCAGAGCTGTTGGCCTCGCCTGGGCCCAGGCTCTGGGAAGGGGTGCCCTCTGGATCCTGCTGTGGCCTCACTTCCCTGGGAACTCATCCTGTGTGGGGAGGCAGCTCCAACAGCTTGACCAGACCTAGACCTGGGCCAAAAGGGCAGCCAGGGGCTGCTCATCACCCAGTCCTGGCCATTTTCTTGCCTGAGGCTCAAGAGGCCCAGGGAGCAATGGGAGGGGGCTCCATGGAGGAGGTGTCCCAAGCTTTGAATACCCCCAGAGACCTTTTCTCTCCCATACCATCACTGAGTGGCTTGTGATTCTGGGATGGACCCTCGCAGCAGGTGCAAGAGACAGAGCCCCCAAGCCTCTGCCCCAAGGGGCCCACAAAGGGGAGAAGGGCCAGCCCTACATCTTCAGCTCCCATAGCGCTGGCTCAGGAAGAAACCCCAAGCAGCATTCAGCACACCCCAAGGGACAACCCCATCATATGACATGCCACCCTCTCCATGCCCAACCTAAGATTGTGTGGGTTTTTTAATTAAAAATGTTAAAAGTTTTAAACATGAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 27951, GC content failed 640, low tm 5479, high tm 16732, high any compl 9, high end compl 36, high repeat similarity 64, long poly-x seq 21, ok 4970
PRIMER_RIGHT_EXPLAIN=considered 27819, GC content failed 933, low tm 5556, high tm 16248, high any compl 4, high end compl 16, high repeat similarity 22, long poly-x seq 39, ok 5001
PRIMER_PAIR_EXPLAIN=considered 1676, unacceptable product size 1622, high end compl 9, ok 45
PRIMER_PAIR_PENALTY=0.5458
PRIMER_LEFT_PENALTY=0.349674
PRIMER_RIGHT_PENALTY=0.196132
PRIMER_LEFT_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_SEQUENCE=GATGGTATGGGAGAGAAAAGGTC
PRIMER_LEFT=1990,23
PRIMER_RIGHT=2169,23
PRIMER_LEFT_TM=60.350
PRIMER_RIGHT_TM=60.196
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_PAIR_MISPRIMING_SCORE=21.00, reverse MER21B a consensus
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.7082
PRIMER_LEFT_1_PENALTY=0.349674
PRIMER_RIGHT_1_PENALTY=0.358533
PRIMER_LEFT_1_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_1_SEQUENCE=CAGTGATGGTATGGGAGAGAAAA
PRIMER_LEFT_1=1990,23
PRIMER_RIGHT_1=2173,23
PRIMER_LEFT_1_TM=60.350
PRIMER_RIGHT_1_TM=60.359
PRIMER_LEFT_1_GC_PERCENT=52.174
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, reverse MER4C a consensus
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.9833
PRIMER_LEFT_2_PENALTY=0.922638
PRIMER_RIGHT_2_PENALTY=0.060618
PRIMER_LEFT_2_SEQUENCE=AAAGCATCTACCTCTTAGCCCCT
PRIMER_RIGHT_2_SEQUENCE=ATGTAGTCGAAGAGCATCTCAGC
PRIMER_LEFT_2=138,23
PRIMER_RIGHT_2=547,23
PRIMER_LEFT_2_TM=60.923
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, reverse MER34 a consensus
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=410
PRIMER_PAIR_PENALTY_3=1.0406
PRIMER_LEFT_3_PENALTY=0.349674
PRIMER_RIGHT_3_PENALTY=0.690888
PRIMER_LEFT_3_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_3_SEQUENCE=AGAGAAAAGGTCTCTGGGGGTAT
PRIMER_LEFT_3=1990,23
PRIMER_RIGHT_3=2158,23
PRIMER_LEFT_3_TM=60.350
PRIMER_RIGHT_3_TM=60.691
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=1.0450
PRIMER_LEFT_4_PENALTY=0.984430
PRIMER_RIGHT_4_PENALTY=0.060618
PRIMER_LEFT_4_SEQUENCE=CAGACAGCCTTGACTCTGGTAGA
PRIMER_RIGHT_4_SEQUENCE=ATGTAGTCGAAGAGCATCTCAGC
PRIMER_LEFT_4=198,23
PRIMER_RIGHT_4=547,23
PRIMER_LEFT_4_TM=60.984
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_4_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=350
PRIMER_PAIR_PENALTY_5=1.1419
PRIMER_LEFT_5_PENALTY=1.081328
PRIMER_RIGHT_5_PENALTY=0.060618
PRIMER_LEFT_5_SEQUENCE=ACAGCCTTGACTCTGGTAGAGC
PRIMER_RIGHT_5_SEQUENCE=ATGTAGTCGAAGAGCATCTCAGC
PRIMER_LEFT_5=201,22
PRIMER_RIGHT_5=547,23
PRIMER_LEFT_5_TM=60.081
PRIMER_RIGHT_5_TM=60.061
PRIMER_LEFT_5_GC_PERCENT=54.545
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, MSTc consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=8.2000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=347
PRIMER_PAIR_PENALTY_6=1.1753
PRIMER_LEFT_6_PENALTY=1.114715
PRIMER_RIGHT_6_PENALTY=0.060618
PRIMER_LEFT_6_SEQUENCE=AAAGCATCTACCTCTTAGCCCC
PRIMER_RIGHT_6_SEQUENCE=ATGTAGTCGAAGAGCATCTCAGC
PRIMER_LEFT_6=138,22
PRIMER_RIGHT_6=547,23
PRIMER_LEFT_6_TM=60.115
PRIMER_RIGHT_6_TM=60.061
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=47.826
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_6_MISPRIMING_SCORE=22.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_6_END_STABILITY=12.4000
PRIMER_RIGHT_6_END_STABILITY=8.2000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=410
PRIMER_PAIR_PENALTY_7=1.2100
PRIMER_LEFT_7_PENALTY=0.349674
PRIMER_RIGHT_7_PENALTY=0.860355
PRIMER_LEFT_7_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_7_SEQUENCE=TCAGTGATGGTATGGGAGAGAAA
PRIMER_LEFT_7=1990,23
PRIMER_RIGHT_7=2174,23
PRIMER_LEFT_7_TM=60.350
PRIMER_RIGHT_7_TM=60.860
PRIMER_LEFT_7_GC_PERCENT=52.174
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_7_END_STABILITY=5.4000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=185
PRIMER_PAIR_PENALTY_8=1.2253
PRIMER_LEFT_8_PENALTY=1.029148
PRIMER_RIGHT_8_PENALTY=0.196132
PRIMER_LEFT_8_SEQUENCE=CACTCAGGACTTTGATGCATTTC
PRIMER_RIGHT_8_SEQUENCE=GATGGTATGGGAGAGAAAAGGTC
PRIMER_LEFT_8=1853,23
PRIMER_RIGHT_8=2169,23
PRIMER_LEFT_8_TM=61.029
PRIMER_RIGHT_8_TM=60.196
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=2.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_8_END_STABILITY=6.9000
PRIMER_RIGHT_8_END_STABILITY=7.6000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=317
PRIMER_PAIR_PENALTY_9=1.2253
PRIMER_LEFT_9_PENALTY=1.029148
PRIMER_RIGHT_9_PENALTY=0.196132
PRIMER_LEFT_9_SEQUENCE=TCACTCAGGACTTTGATGCATTT
PRIMER_RIGHT_9_SEQUENCE=GATGGTATGGGAGAGAAAAGGTC
PRIMER_LEFT_9=1852,23
PRIMER_RIGHT_9=2169,23
PRIMER_LEFT_9_TM=61.029
PRIMER_RIGHT_9_TM=60.196
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=47.826
PRIMER_LEFT_9_SELF_ANY=6.00
PRIMER_RIGHT_9_SELF_ANY=2.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=1.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse MER21B a consensus
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_9_END_STABILITY=7.2000
PRIMER_RIGHT_9_END_STABILITY=7.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=318
PRIMER_PAIR_PENALTY_10=1.2785
PRIMER_LEFT_10_PENALTY=1.217855
PRIMER_RIGHT_10_PENALTY=0.060618
PRIMER_LEFT_10_SEQUENCE=AAGCATCTACCTCTTAGCCCCT
PRIMER_RIGHT_10_SEQUENCE=ATGTAGTCGAAGAGCATCTCAGC
PRIMER_LEFT_10=139,22
PRIMER_RIGHT_10=547,23
PRIMER_LEFT_10_TM=59.782
PRIMER_RIGHT_10_TM=60.061
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=3.00
PRIMER_RIGHT_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, LTR3 LTR from human DNA related to mouse mammary tumor virus (MMTV) 3' LTR
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_10_MISPRIMING_SCORE=23.00, reverse L1MA9 3'-end of L1 repeat (subfamily L1MA9) - a consensus sequence
PRIMER_LEFT_10_END_STABILITY=10.9000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=409
PRIMER_PAIR_PENALTY_11=1.3026
PRIMER_LEFT_11_PENALTY=1.242013
PRIMER_RIGHT_11_PENALTY=0.060618
PRIMER_LEFT_11_SEQUENCE=AGCATCTACCTCTTAGCCCCTC
PRIMER_RIGHT_11_SEQUENCE=ATGTAGTCGAAGAGCATCTCAGC
PRIMER_LEFT_11=140,22
PRIMER_RIGHT_11=547,23
PRIMER_LEFT_11_TM=60.242
PRIMER_RIGHT_11_TM=60.061
PRIMER_LEFT_11_GC_PERCENT=54.545
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MER21B a consensus
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, reverse L1MA5 3'-end of L1 repeat (subfamily L1MA5) - a consensus sequence
PRIMER_LEFT_11_END_STABILITY=9.4000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=408
PRIMER_PAIR_PENALTY_12=1.3602
PRIMER_LEFT_12_PENALTY=0.349674
PRIMER_RIGHT_12_PENALTY=1.010545
PRIMER_LEFT_12_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_12_SEQUENCE=CACTCAGTGATGGTATGGGAGA
PRIMER_LEFT_12=1990,23
PRIMER_RIGHT_12=2177,22
PRIMER_LEFT_12_TM=60.350
PRIMER_RIGHT_12_TM=59.989
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_12_END_STABILITY=5.4000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=188
PRIMER_PAIR_PENALTY_13=1.3606
PRIMER_LEFT_13_PENALTY=0.165047
PRIMER_RIGHT_13_PENALTY=1.195574
PRIMER_LEFT_13_SEQUENCE=CCACGATGATCTCCTGCTACTAC
PRIMER_RIGHT_13_SEQUENCE=AGCATACAGGCCTTCTTACAGG
PRIMER_LEFT_13=793,23
PRIMER_RIGHT_13=1558,22
PRIMER_LEFT_13_TM=60.165
PRIMER_RIGHT_13_TM=59.804
PRIMER_LEFT_13_GC_PERCENT=52.174
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=5.1000
PRIMER_RIGHT_13_END_STABILITY=7.9000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=766
PRIMER_PAIR_PENALTY_14=1.3648
PRIMER_LEFT_14_PENALTY=0.349674
PRIMER_RIGHT_14_PENALTY=1.015139
PRIMER_LEFT_14_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_14_SEQUENCE=GAGAAAAGGTCTCTGGGGGTATT
PRIMER_LEFT_14=1990,23
PRIMER_RIGHT_14=2157,23
PRIMER_LEFT_14_TM=60.350
PRIMER_RIGHT_14_TM=61.015
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=5.4000
PRIMER_RIGHT_14_END_STABILITY=5.6000
PRIMER_PAIR_14_COMPL_ANY=6.00
PRIMER_PAIR_14_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_14=168
PRIMER_PAIR_PENALTY_15=1.3648
PRIMER_LEFT_15_PENALTY=0.349674
PRIMER_RIGHT_15_PENALTY=1.015139
PRIMER_LEFT_15_SEQUENCE=CAACAGCTTGACCAGACCTAGAC
PRIMER_RIGHT_15_SEQUENCE=AGAAAAGGTCTCTGGGGGTATTC
PRIMER_LEFT_15=1990,23
PRIMER_RIGHT_15=2156,23
PRIMER_LEFT_15_TM=60.350
PRIMER_RIGHT_15_TM=61.015
PRIMER_LEFT_15_GC_PERCENT=52.174
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=3.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_LEFT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=5.4000
PRIMER_RIGHT_15_END_STABILITY=5.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=167
PRIMER_PAIR_PENALTY_16=1.3788
PRIMER_LEFT_16_PENALTY=1.029148
PRIMER_RIGHT_16_PENALTY=0.349674
PRIMER_LEFT_16_SEQUENCE=CACTCAGGACTTTGATGCATTTC
PRIMER_RIGHT_16_SEQUENCE=GTCTAGGTCTGGTCAAGCTGTTG
PRIMER_LEFT_16=1853,23
PRIMER_RIGHT_16=2012,23
PRIMER_LEFT_16_TM=61.029
PRIMER_RIGHT_16_TM=60.350
PRIMER_LEFT_16_GC_PERCENT=43.478
PRIMER_RIGHT_16_GC_PERCENT=52.174
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=3.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=6.9000
PRIMER_RIGHT_16_END_STABILITY=7.0000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=160
PRIMER_PAIR_PENALTY_17=1.3788
PRIMER_LEFT_17_PENALTY=1.029148
PRIMER_RIGHT_17_PENALTY=0.349674
PRIMER_LEFT_17_SEQUENCE=TCACTCAGGACTTTGATGCATTT
PRIMER_RIGHT_17_SEQUENCE=GTCTAGGTCTGGTCAAGCTGTTG
PRIMER_LEFT_17=1852,23
PRIMER_RIGHT_17=2012,23
PRIMER_LEFT_17_TM=61.029
PRIMER_RIGHT_17_TM=60.350
PRIMER_LEFT_17_GC_PERCENT=39.130
PRIMER_RIGHT_17_GC_PERCENT=52.174
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=2.00
PRIMER_RIGHT_17_SELF_END=3.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=22.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_17_END_STABILITY=7.2000
PRIMER_RIGHT_17_END_STABILITY=7.0000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_17=161
PRIMER_PAIR_PENALTY_18=1.3877
PRIMER_LEFT_18_PENALTY=1.029148
PRIMER_RIGHT_18_PENALTY=0.358533
PRIMER_LEFT_18_SEQUENCE=CACTCAGGACTTTGATGCATTTC
PRIMER_RIGHT_18_SEQUENCE=CAGTGATGGTATGGGAGAGAAAA
PRIMER_LEFT_18=1853,23
PRIMER_RIGHT_18=2173,23
PRIMER_LEFT_18_TM=61.029
PRIMER_RIGHT_18_TM=60.359
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=7.3000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_18=321
PRIMER_PAIR_PENALTY_19=1.3877
PRIMER_LEFT_19_PENALTY=1.029148
PRIMER_RIGHT_19_PENALTY=0.358533
PRIMER_LEFT_19_SEQUENCE=TCACTCAGGACTTTGATGCATTT
PRIMER_RIGHT_19_SEQUENCE=CAGTGATGGTATGGGAGAGAAAA
PRIMER_LEFT_19=1852,23
PRIMER_RIGHT_19=2173,23
PRIMER_LEFT_19_TM=61.029
PRIMER_RIGHT_19_TM=60.359
PRIMER_LEFT_19_GC_PERCENT=39.130
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, L1ME3a 3'-end of L1 repeat (subfamily L1ME3a) - a consensus sequence
PRIMER_RIGHT_19_MISPRIMING_SCORE=12.00, reverse MER4C a consensus
PRIMER_PAIR_19_MISPRIMING_SCORE=22.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_LEFT_19_END_STABILITY=7.2000
PRIMER_RIGHT_19_END_STABILITY=7.3000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_19=322
=