PRIMER_SEQUENCE_ID=NM_145260
SEQUENCE=GCTCGGCCTCTCCCCGGCACGTTCTCAGCTGCTCCTGGTTCAGACCCAGCGAGGGAGCCGCGAGCGAGGCTCACCGTCCCCGGCGTGCAGGATCCGGGGCTGCTGAGCGCTCGCTCCCGCGTGTCCGGCGCTTGGAGTCCCCGCGGCAGGAGAGGAGTCGGGACACTAGAGCTCCAGGGGCGCCTGTGGGCTCCAGGGCCTCCGGCTTCCCCAGTCCCCTTCAGCTAAAGCCCCAGAGACGTGCTCAGCCCCAGGACCTCTGCGGAACAAGATCCGGATTGAGAAGCCACTGCAACTACCGAAATGGGCAGCAAAACCTTGCCGGCGCCGGTGCCTATCCACCCTTCCCTGCAGCTCACCAACTACTCCTTCCTTCAGGCAGTGAACGGCCTGCCCACAGTGCCTTCGGACCATCTGCCCAACCTGTATGGTTTCAGCGCGTTGCACGCTGTGCACCTGCATCAGTGGACGCTGGGCTACCCGGCCATGCACTTGCCGCGCTCTTCTTTCTCCAAAGTGCCGGGCACGGTGTCCAGCTTGGTGGATGCGCGCTTCCAGCTGCCCGCCTTTCCCTGGTTCCCTCATGTCATTCAACCCAAGCCCGAGATCACCGCTGGAGGCAGCGTTCCAGCGCTCAAGACCAAGCCGCGCTTTGATTTTGCCAACCTGGCCTTGGCAGCAACGCAAGAAGATCCGGCCAAGCTCGGTCGCGGGGAGGGCCCAGGCTCCCCTGCAGGTGGGCTGGGTGCCCTCCTCGACGTGACCAAGCTGTCTCCAGAAAAGAAGCCCACAAGGGGACGTCTGCCTTCCAAGACCAAGAAGGAATTCGTCTGCAAGTTCTGTGGCCGCCACTTCACCAAGTCCTACAACCTACTTATCCATGAGCGGACGCACACCGACGAGCGGCCCTACACCTGTGACATCTGCCACAAAGCCTTCCGGAGGCAAGACCACCTGCGAGACCACAGATATATTCACTCCAAAGAGAAGCCCTTCAAGTGTCAAGAGTGTGGGAAAGGATTCTGCCAGTCCAGGACTCTCGCTGTCCACAAGACGCTACACTCACAGGTGAAGGAGCTCAAAACCTCCAAGATCAAATGCTAAATAGAACCTGTGGGTCACAAGGACCCTAGGCCCAGCGGCCCTCTCCTCCATAGGGACCAGAAGCCTGACTCTGGCGGGCAGCGGGAGAGGCGCTCGCTCGGGACCTTCCACCTCTCCAACATTGTCCCCTGGGTCCCTGGCGCACGCGGCACTTCAGAGCCCCGCCCGGGGCCGCGACCCCGACCGTCCCTGCTGCTCCCCTAGGACGCGGCTAAACTCTTGGCCAAAAGGCGGTACTCACGTGGCGGAAGGGAAACTGCATTAAAAAAACGAAAGCTCCGCGGAGCCGCAGCGGCGCCTCTCCCAGAAGTATTACTTTTTCTATTGTTATTTTATACGTTTTCTTTTTTATTTTTGTCTTTGACCATATAAGCTTGTAACTCTGACTGCGGAGAGTGAGTGGAGAGAGGAGAGGCAACGAAGTCCTTAGCACTGCAGCTCCCACCTCCTCCTCCCGCCCCTGCCCCCACCCCCGGAGCCTGCAAAAGTGTAATCCCTGTATCTGCTTCAGCCTCCTGCCCTAGGGACCGCGGGGCAGCCCCCTCCCAGCTCTTGCAGCTCAGCTGGCGGCCGTGGGCTCCGGGACTAGGAGAGCGGGACGGGGACCTCGGGCCTGGGGTCTGCAGGGGGCCCGGCCTCCGCTGCCGCGACACTGCGAGGGAGCAGCCAGCCAAGAGCCGGGCGTTATATTGCGATTGGCACTTTATGCTGACCATCGGTAACGGACATTTATCACTGGAGTTTTTTTTTTTTTTTAACTATTAAATAAACGGTTATTTTACAGGCAAAAAAAAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 21549, GC content failed 1971, low tm 4265, high tm 11862, high any compl 1, high end compl 25, high repeat similarity 21, long poly-x seq 36, ok 3368
PRIMER_RIGHT_EXPLAIN=considered 21316, GC content failed 2278, low tm 4420, high tm 11180, high any compl 1, high end compl 3, high repeat similarity 5, long poly-x seq 67, ok 3362
PRIMER_PAIR_EXPLAIN=considered 294, unacceptable product size 243, high end compl 7, ok 44
PRIMER_PAIR_PENALTY=0.1352
PRIMER_LEFT_PENALTY=0.012719
PRIMER_RIGHT_PENALTY=0.122456
PRIMER_LEFT_SEQUENCE=CTCAAAACCTCCAAGATCAAATG
PRIMER_RIGHT_SEQUENCE=TCCAGTGATAAATGTCCGTTACC
PRIMER_LEFT=1077,23
PRIMER_RIGHT=1843,23
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=39.130
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=767
PRIMER_PAIR_PENALTY_1=0.1640
PRIMER_LEFT_1_PENALTY=0.163841
PRIMER_RIGHT_1_PENALTY=0.000148
PRIMER_LEFT_1_SEQUENCE=AGCTCACCAACTACTCCTTCCTT
PRIMER_RIGHT_1_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_1=352,23
PRIMER_RIGHT_1=975,23
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=624
PRIMER_PAIR_PENALTY_2=0.2034
PRIMER_LEFT_2_PENALTY=0.203244
PRIMER_RIGHT_2_PENALTY=0.000148
PRIMER_LEFT_2_SEQUENCE=CTCACCAACTACTCCTTCCTTCA
PRIMER_RIGHT_2_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_2=354,23
PRIMER_RIGHT_2=975,23
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=622
PRIMER_PAIR_PENALTY_3=0.2034
PRIMER_LEFT_3_PENALTY=0.203244
PRIMER_RIGHT_3_PENALTY=0.000148
PRIMER_LEFT_3_SEQUENCE=TCACCAACTACTCCTTCCTTCAG
PRIMER_RIGHT_3_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_3=355,23
PRIMER_RIGHT_3=975,23
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_RIGHT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=621
PRIMER_PAIR_PENALTY_4=0.3167
PRIMER_LEFT_4_PENALTY=0.316570
PRIMER_RIGHT_4_PENALTY=0.000148
PRIMER_LEFT_4_SEQUENCE=GATTGAGAAGCCACTGCAACTAC
PRIMER_RIGHT_4_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_4=276,23
PRIMER_RIGHT_4=975,23
PRIMER_LEFT_4_TM=60.317
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_4_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=700
PRIMER_PAIR_PENALTY_5=0.4950
PRIMER_LEFT_5_PENALTY=0.012719
PRIMER_RIGHT_5_PENALTY=0.482233
PRIMER_LEFT_5_SEQUENCE=CTCAAAACCTCCAAGATCAAATG
PRIMER_RIGHT_5_SEQUENCE=ACTCTCCGCAGTCAGAGTTACAA
PRIMER_LEFT_5=1077,23
PRIMER_RIGHT_5=1500,23
PRIMER_LEFT_5_TM=59.987
PRIMER_RIGHT_5_TM=60.482
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=47.826
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MER44A Nonautonomous DNA transposon
PRIMER_PAIR_5_MISPRIMING_SCORE=22.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_5_END_STABILITY=7.2000
PRIMER_RIGHT_5_END_STABILITY=6.0000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=424
PRIMER_PAIR_PENALTY_6=0.4966
PRIMER_LEFT_6_PENALTY=0.012719
PRIMER_RIGHT_6_PENALTY=0.483834
PRIMER_LEFT_6_SEQUENCE=CTCAAAACCTCCAAGATCAAATG
PRIMER_RIGHT_6_SEQUENCE=GTGATAAATGTCCGTTACCGATG
PRIMER_LEFT_6=1077,23
PRIMER_RIGHT_6=1839,23
PRIMER_LEFT_6_TM=59.987
PRIMER_RIGHT_6_TM=60.484
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=7.2000
PRIMER_RIGHT_6_END_STABILITY=8.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=763
PRIMER_PAIR_PENALTY_7=0.5228
PRIMER_LEFT_7_PENALTY=0.400326
PRIMER_RIGHT_7_PENALTY=0.122456
PRIMER_LEFT_7_SEQUENCE=CCTGCAAAAGTGTAATCCCTGTA
PRIMER_RIGHT_7_SEQUENCE=TCCAGTGATAAATGTCCGTTACC
PRIMER_LEFT_7=1582,23
PRIMER_RIGHT_7=1843,23
PRIMER_LEFT_7_TM=60.400
PRIMER_RIGHT_7_TM=60.122
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=3.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_7_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=5.7000
PRIMER_RIGHT_7_END_STABILITY=7.2000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=262
PRIMER_PAIR_PENALTY_8=0.5705
PRIMER_LEFT_8_PENALTY=0.163841
PRIMER_RIGHT_8_PENALTY=0.406645
PRIMER_LEFT_8_SEQUENCE=AGCTCACCAACTACTCCTTCCTT
PRIMER_RIGHT_8_SEQUENCE=ATATATCTGTGGTCTCGCAGGTG
PRIMER_LEFT_8=352,23
PRIMER_RIGHT_8=973,23
PRIMER_LEFT_8_TM=59.836
PRIMER_RIGHT_8_TM=60.407
PRIMER_LEFT_8_GC_PERCENT=47.826
PRIMER_RIGHT_8_GC_PERCENT=47.826
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_8_MISPRIMING_SCORE=21.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_8_END_STABILITY=8.2000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_8=622
PRIMER_PAIR_PENALTY_9=0.5886
PRIMER_LEFT_9_PENALTY=0.012719
PRIMER_RIGHT_9_PENALTY=0.575907
PRIMER_LEFT_9_SEQUENCE=CTCAAAACCTCCAAGATCAAATG
PRIMER_RIGHT_9_SEQUENCE=AAAACTCCAGTGATAAATGTCCG
PRIMER_LEFT_9=1077,23
PRIMER_RIGHT_9=1848,23
PRIMER_LEFT_9_TM=59.987
PRIMER_RIGHT_9_TM=59.424
PRIMER_LEFT_9_GC_PERCENT=39.130
PRIMER_RIGHT_9_GC_PERCENT=39.130
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_9_END_STABILITY=7.2000
PRIMER_RIGHT_9_END_STABILITY=9.6000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=772
PRIMER_PAIR_PENALTY_10=0.6099
PRIMER_LEFT_10_PENALTY=0.203244
PRIMER_RIGHT_10_PENALTY=0.406645
PRIMER_LEFT_10_SEQUENCE=TCACCAACTACTCCTTCCTTCAG
PRIMER_RIGHT_10_SEQUENCE=ATATATCTGTGGTCTCGCAGGTG
PRIMER_LEFT_10=355,23
PRIMER_RIGHT_10=973,23
PRIMER_LEFT_10_TM=59.797
PRIMER_RIGHT_10_TM=60.407
PRIMER_LEFT_10_GC_PERCENT=47.826
PRIMER_RIGHT_10_GC_PERCENT=47.826
PRIMER_LEFT_10_SELF_ANY=2.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=2.00
PRIMER_RIGHT_10_SELF_END=2.00
PRIMER_LEFT_10_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.0000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=619
PRIMER_PAIR_PENALTY_11=0.6099
PRIMER_LEFT_11_PENALTY=0.203244
PRIMER_RIGHT_11_PENALTY=0.406645
PRIMER_LEFT_11_SEQUENCE=CTCACCAACTACTCCTTCCTTCA
PRIMER_RIGHT_11_SEQUENCE=ATATATCTGTGGTCTCGCAGGTG
PRIMER_LEFT_11=354,23
PRIMER_RIGHT_11=973,23
PRIMER_LEFT_11_TM=59.797
PRIMER_RIGHT_11_TM=60.407
PRIMER_LEFT_11_GC_PERCENT=47.826
PRIMER_RIGHT_11_GC_PERCENT=47.826
PRIMER_LEFT_11_SELF_ANY=2.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=1.00
PRIMER_RIGHT_11_SELF_END=2.00
PRIMER_LEFT_11_MISPRIMING_SCORE=11.00, MARINER1 Human mariner
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_11_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=7.9000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=620
PRIMER_PAIR_PENALTY_12=0.6176
PRIMER_LEFT_12_PENALTY=0.012719
PRIMER_RIGHT_12_PENALTY=0.604859
PRIMER_LEFT_12_SEQUENCE=CTCAAAACCTCCAAGATCAAATG
PRIMER_RIGHT_12_SEQUENCE=CTCTCCGCAGTCAGAGTTACAAG
PRIMER_LEFT_12=1077,23
PRIMER_RIGHT_12=1499,23
PRIMER_LEFT_12_TM=59.987
PRIMER_RIGHT_12_TM=60.605
PRIMER_LEFT_12_GC_PERCENT=39.130
PRIMER_RIGHT_12_GC_PERCENT=52.174
PRIMER_LEFT_12_SELF_ANY=4.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_12_MISPRIMING_SCORE=11.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=7.2000
PRIMER_RIGHT_12_END_STABILITY=6.7000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=423
PRIMER_PAIR_PENALTY_13=0.6314
PRIMER_LEFT_13_PENALTY=0.631237
PRIMER_RIGHT_13_PENALTY=0.000148
PRIMER_LEFT_13_SEQUENCE=AAGAAGGAATTCGTCTGCAAGTT
PRIMER_RIGHT_13_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_13=816,23
PRIMER_RIGHT_13=975,23
PRIMER_LEFT_13_TM=60.631
PRIMER_RIGHT_13_TM=60.000
PRIMER_LEFT_13_GC_PERCENT=39.130
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=6.00
PRIMER_LEFT_13_SELF_END=3.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_13_MISPRIMING_SCORE=24.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=9.7000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=160
PRIMER_PAIR_PENALTY_14=0.6489
PRIMER_LEFT_14_PENALTY=0.648733
PRIMER_RIGHT_14_PENALTY=0.000148
PRIMER_LEFT_14_SEQUENCE=GCTCACCAACTACTCCTTCCTTC
PRIMER_RIGHT_14_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_14=353,23
PRIMER_RIGHT_14=975,23
PRIMER_LEFT_14_TM=60.649
PRIMER_RIGHT_14_TM=60.000
PRIMER_LEFT_14_GC_PERCENT=52.174
PRIMER_RIGHT_14_GC_PERCENT=47.826
PRIMER_LEFT_14_SELF_ANY=2.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT1R MLT1-Mammalian LTR retrotransposon internal sequence - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=8.2000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_14=623
PRIMER_PAIR_PENALTY_15=0.7101
PRIMER_LEFT_15_PENALTY=0.012719
PRIMER_RIGHT_15_PENALTY=0.697346
PRIMER_LEFT_15_SEQUENCE=CTCAAAACCTCCAAGATCAAATG
PRIMER_RIGHT_15_SEQUENCE=GCTGAAGCAGATACAGGGATTAC
PRIMER_LEFT_15=1077,23
PRIMER_RIGHT_15=1615,23
PRIMER_LEFT_15_TM=59.987
PRIMER_RIGHT_15_TM=59.303
PRIMER_LEFT_15_GC_PERCENT=39.130
PRIMER_RIGHT_15_GC_PERCENT=47.826
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_RIGHT_15_MISPRIMING_SCORE=12.00, MER31 a consensus
PRIMER_PAIR_15_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=7.2000
PRIMER_RIGHT_15_END_STABILITY=5.6000
PRIMER_PAIR_15_COMPL_ANY=3.00
PRIMER_PAIR_15_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_15=539
PRIMER_PAIR_PENALTY_16=0.7220
PRIMER_LEFT_16_PENALTY=0.163841
PRIMER_RIGHT_16_PENALTY=0.558207
PRIMER_LEFT_16_SEQUENCE=AGCTCACCAACTACTCCTTCCTT
PRIMER_RIGHT_16_SEQUENCE=CTTGTGACCCACAGGTTCTATTT
PRIMER_LEFT_16=352,23
PRIMER_RIGHT_16=1124,23
PRIMER_LEFT_16_TM=59.836
PRIMER_RIGHT_16_TM=59.442
PRIMER_LEFT_16_GC_PERCENT=47.826
PRIMER_RIGHT_16_GC_PERCENT=43.478
PRIMER_LEFT_16_SELF_ANY=4.00
PRIMER_RIGHT_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_16_MISPRIMING_SCORE=20.00, MER4C a consensus
PRIMER_LEFT_16_END_STABILITY=8.2000
PRIMER_RIGHT_16_END_STABILITY=6.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=773
PRIMER_PAIR_PENALTY_17=0.7256
PRIMER_LEFT_17_PENALTY=0.725435
PRIMER_RIGHT_17_PENALTY=0.000148
PRIMER_LEFT_17_SEQUENCE=AACCTACTTATCCATGAGCGGAC
PRIMER_RIGHT_17_SEQUENCE=GAATATATCTGTGGTCTCGCAGG
PRIMER_LEFT_17=867,23
PRIMER_RIGHT_17=975,23
PRIMER_LEFT_17_TM=60.725
PRIMER_RIGHT_17_TM=60.000
PRIMER_LEFT_17_GC_PERCENT=47.826
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=4.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=1.00
PRIMER_RIGHT_17_SELF_END=1.00
PRIMER_LEFT_17_MISPRIMING_SCORE=11.00, L1MA2 3'-end of L1 repeat (subfamily L1MA2) - a consensus sequence
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=9.6000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=109
PRIMER_PAIR_PENALTY_18=0.7273
PRIMER_LEFT_18_PENALTY=0.604859
PRIMER_RIGHT_18_PENALTY=0.122456
PRIMER_LEFT_18_SEQUENCE=CTTGTAACTCTGACTGCGGAGAG
PRIMER_RIGHT_18_SEQUENCE=TCCAGTGATAAATGTCCGTTACC
PRIMER_LEFT_18=1477,23
PRIMER_RIGHT_18=1843,23
PRIMER_LEFT_18_TM=60.605
PRIMER_RIGHT_18_TM=60.122
PRIMER_LEFT_18_GC_PERCENT=52.174
PRIMER_RIGHT_18_GC_PERCENT=43.478
PRIMER_LEFT_18_SELF_ANY=4.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, reverse L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_PAIR_18_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=7.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_18=367
PRIMER_PAIR_PENALTY_19=0.7615
PRIMER_LEFT_19_PENALTY=0.203244
PRIMER_RIGHT_19_PENALTY=0.558207
PRIMER_LEFT_19_SEQUENCE=TCACCAACTACTCCTTCCTTCAG
PRIMER_RIGHT_19_SEQUENCE=CTTGTGACCCACAGGTTCTATTT
PRIMER_LEFT_19=355,23
PRIMER_RIGHT_19=1124,23
PRIMER_LEFT_19_TM=59.797
PRIMER_RIGHT_19_TM=59.442
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=43.478
PRIMER_LEFT_19_SELF_ANY=2.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_MISPRIMING_SCORE=11.00, MER28 Nonautonomous DNA transposon
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_19_MISPRIMING_SCORE=21.00, reverse MER21 a consensus
PRIMER_LEFT_19_END_STABILITY=7.0000
PRIMER_RIGHT_19_END_STABILITY=6.2000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=770
=