PRIMER_SEQUENCE_ID=NM_153827
SEQUENCE=GGCTGGCTCCGGGGAGATAGCGCCTGTCAGTCGGTGGGTCGGTCCTCGCGCCGGCCCTCCCCCTCCCCGGTCTCCGGGGGAGGCGCGGTGGAGTCCGCCCCCGGGGTTCTCCGATGGGGGAGAAGCGGCGACGGCGGCAGTGGAGTAACCGAGCCGGAGCGTGAGCGGCCCCGGTGCCCCGTTCCCCACGGAGGCCATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCCGCCCTGCGGGACCCTGCTGGGATCTTTGAGCTTGTGGAGGTGGTCGGCAATGGAACCTACGGACAGGTGTACAAGGGTCGGCATGTCAAGACGGGGCAGCTGGCTGCCATCAAGGTCATGGATGTCACGGAGGACGAGGAGGAAGAGATCAAACAGGAGATCAACATGCTGAAAAAGTACTCTCACCACCGCAACATCGCCACCTACTACGGAGCCTTCATCAAGAAGAGCCCCCCGGGAAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGTGGTGCTGGTTCAGTGACTGACCTGGTAAAGAACACAAAAGGCAACGCCCTGAAGGAGGACTGTATCGCCTATATCTGCAGGGAGATCCTCAGGGGTCTGGCCCATCTCCATGCCCACAAGGTGATCCATCGAGACATCAAGGGGCAGAATGTGCTGCTGACAGAGAATGCTGAGGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACCGTGGGCAGACGGAACACTTTCATTGGGACTCCCTACTGGATGGCTCCAGAGGTCATCGCCTGTGATGAGAACCCTGATGCCACCTATGATTACAGGAGTGATATTTGGTCTCTAGGAATCACAGCCATCGAGATGGCAGAGGGAGCCCCCCCTCTGTGTGACATGCACCCCATGCGAGCCCTCTTCCTCATTCCTCGGAACCCTCCGCCCAGGCTCAAGTCCAAGAAGTGGTCTAAGAAGTTCATTGACTTCATTGACACATGTCTCATCAAGACTTACCTGAGCCGCCCACCCACGGAGCAGCTACTGAAGTTTCCCTTCATCCGGGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTTAAGGACCACATTGACCGATCCCGGAAGAAGCGGGGTGAGAAAGAGGAGACAGAATATGAGTACAGCGGCAGCGAGGAGGAAGATGACAGCCATGGAGAGGAAGGAGAGCCAAGCTCCATCATGAACGTGCCTGGAGAGTCGACTCTACGCCGGGAGTTTCTCCGGCTCCAGCAGGAAAATAAGAGCAACTCAGAGGCTTTAAAACAGCAGCAGCAGCTGCAGCAGCAGCAGCAGCGAGACCCCGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGGCGCATAGAGGAGCAGAAGGAGGAGCGGCGCCGCGTGGAGGAGCAACAGCGGCGGGAGCGGGAGCAGCGGAAGCTGCAGGAGAAGGAGCAGCAGCGGCGGCTGGAGGACATGCAGGCTCTGCGGCGGGAGGAGGAGCGGCGGCAGGCGGAGCGCGAGCAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAACGTCTCCAGAGGCAGCTGCAGCAGGAGCATGCCTACCTCAAGTCCCTGCAGCAGCAGCAACAGCAGCAGCAGCTTCAGAAACAGCAGCAGCAGCAGCTCCTGCCTGGGGACAGGAAGCCCCTGTACCATTATGGTCGGGGCATGAATCCCGCTGACAAACCAGCCTGGGCCCGAGAGGTAGAAGAGAGAACAAGGATGAACAAGCAGCAGAACTCTCCCTTGGCCAAGAGCAAGCCAGGCAGCACGGGGCCTGAGCCCCCCATCCCCCAGGCCTCCCCAGGGCCCCCAGGACCCCTTTCCCAGACTCCTCCTATGCAGAGGCCGGTGGAGCCCCAGGAGGGACCGCACAAGAGCCTGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCAGCCCACCCGAAACCTGGCTGCCTTCCCAGCCTCCCATGACCCCGACCCTGCCATCCCCGCACCCACTGCCACGCCCAGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGACCTGGCCCCAGCCCGAATCCCCCAGCCTGGGTCCGCCCAGATAACGAGGCCCCACCCAAGGTGCCTCAGAGGACCTCATCTATCGCCACTGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCCCAGGCAGTCCGTGCCAGACCTCGCAGCAACTCCGCCTGGCAAATCTATCTGCAAAGGCGGGCAGAGCGGGGCACCCCAAAGCCTCCAGGGCCCCCTGCTCAGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCCCGACCTCAGGAGGAGCGACCCTGGCTGGGAACGCTCGGACAGCGTCCTTCCAGCCTCTCACGGGCACCTCCCCCAGGCTGGCTCACTGGAGCGGAACCGCGTGGGAGTCTCCTCCAAACCGGACAGCTCCCCTGTGCTCTCCCCTGGGAATAAAGCCAAGCCCGACGACCACCGCTCACGGCCAGGCCGGCCCGCAGACTTTGTGTTGCTGAAAGAGCGGACTCTGGACGAGGCCCCTCGGCCTCCCAAGAAGGCCATGGACTACTCGTCGTCCAGCGAGGAGGTGGAAAGCAGTGAGGACGACGAGGAGGAAGGCGAAGGCGGGCCAGCAGAGGGGAGCAGAGATACCCCTGGGGGCCGCAGCGATGGGGATACAGACAGCGTCAGCACCATGGTGGTCCACGACGTCGAGGAGATCACCGGGACCCAGCCCCCATACGGGGGCGGCACCATGGTGGTCCAGCGCACCCCTGAAGAGGAGCGGAACCTGCTGCATGCTGACAGCAATGGGTACACAAACCTGCCTGACGTGGTCCAGCCCAGCCACTCACCCACCGAGAACAGCAAAGGCCAAAGCCCACCCTCGAAGGATGGGAGTGGTGACTACCAGTCTCGTGGGCTGGTAAAGGCCCCTGGCAAGAGCTCGTTCACGATGTTTGTGGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACAGCATCCCCATCACAGCCCTAGTGGGTGGAGAGGGCACTCGGCTCGACCAGCTGCAGTACGACGTGAGGAAGGGTTCTGTGGTCAACGTGAATCCCACCAACACCCGGGCCCACAGTGAGACCCCTGAGATCCGGAAGTACAAGAAGCGATTCAACTCCGAGATCCTCTGTGCAGCCCTTTGGGGGGTCAACCTGCTGGTGGGCACGGAGAACGGGCTGATGTTGCTGGACCGAAGTGGGCAGGGCAAGGTGTATGGACTCATTGGGCGGCGACGCTTCCAGCAGATGGATGTGCTGGAGGGGCTCAACCTGCTCATCACCATCTCAGGGAAAAGGAACAAACTGCGGGTGTATTACCTGTCCTGGCTCCGGAACAAGATTCTGCACAATGACCCAGAAGTGGAGAAGAAGCAGGGCTGGACCACCGTGGGGGACATGGAGGGCTGCGGGCACTACCGTGTTGTGAAATACGAGCGGATTAAGTTCCTGGTCATCGCCCTCAAGAGCTCCGTGGAGGTGTATGCCTGGGCCCCCAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCCGACCTCCCCCACCGCCCTCTGCTGGTCGACCTGACAGTAGAGGAGGGGCAGCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCATGCTGTGGATGTCGACTCGGGGAACAGCTATGACATCTACATCCCTGTGCACATCCAGAGCCAGATCACGCCCCATGCCATCATCTTCCTCCCCAACACCGACGGCATGGAGATGCTGCTGTGCTACGAGGACGAGGGTGTCTACGTCAACACGTACGGGCGCATCATTAAGGATGTGGTGCTGCAGTGGGGGGAGATGCCTACTTCTGTGGCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGAAAGCCATTGAGATCCGCTCTGTGGAGACGGGCCACCTCGACGGGGTCTTCATGCACAAACGAGCTCAGAGGCTCAAGTTCCTGTGTGAGCGGAATGACAAGGTGTTTTTTGCCTCAGTCCGCTCTGGGGGCAGCAGCCAAGTTTACTTCATGACTCTGAACCGTAACTGCATCATGAACTGGTGACGGGGCCCTGGGCTGGGGCTGTCCCACACTGGACCCAGCTCTCCCCCTGCAGCCAGGCTTCCCGGGCCGCCCCTCTTTCCCCTCCCTGGGCTTTTGCTTTTACTGGTTTGATTTCACTGGAGCCTGCTGGGAACGTGACCTCTGACCCCTGATGCTTTCGTGATCACGTGACCATCCTCTTCCCCAACATGTCCTCTTCCCAAAACTGTGCCTGTCCCCAGCTTCTGGGGAGGGACACAGCTTCCCCTTCCCAGGAATTGAGTGGGCCTAGCCCCTCCCCCCTTTTCTCCATTTGAGAGGAGAGTGCTTGGGGCTTGAACCCCTTACCCCACTGCTGCTGACTGGGCAGGGCCCTGGACCCCTTTATTTGCACGTCAGGGGAGCCGGCTCCCCCCTTGAATGTACCAGACCCTGGGGGGGGTCACTGGGCCCTAGATTTTTGGGGGGTCACCAGCCACTCCAGGGGCAGGGACCATTTCTTCATTTTCTGAAAGCACTTTAATGATTCCCCTTCCCCCAAACTCCAGGGAATGGAGGGGGGACCCCGCCAGCCAAAACATTCCCCCCATTCCCGACCCCCCTCTCCTCTTCTAGCCCATGCCCTTCCCCGGTGGAGGGAGGGAGCAGGGAGCCCTCACTCTCCACGCCCCTTGCTTGCATCTGTATATAGTGTGAGCAGCAAGTAACCCTTCTCCTCCCTCCCCCCTCACCCCTCCTCAATGTAGTGGCCTTGGATATCCTGTTTGTTAATAAAGACAATTCAACCAGCTCCCACCAAAAAAAAAAAAAAAAAA
PRIMER_OPT_SIZE=23
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=26
PRIMER_MISPRIMING_LIBRARY=Human_rep_in_fasta.txt
PRIMER_NUM_RETURN=20
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-999
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 56489, GC content failed 2575, low tm 12234, high tm 31237, high any compl 1, high end compl 40, high repeat similarity 49, long poly-x seq 283, ok 10070
PRIMER_RIGHT_EXPLAIN=considered 56309, GC content failed 2163, low tm 12577, high tm 31033, high end compl 6, high repeat similarity 17, long poly-x seq 305, ok 10208
PRIMER_PAIR_EXPLAIN=considered 4438, unacceptable product size 4365, high end compl 9, ok 64
PRIMER_PAIR_PENALTY=0.0820
PRIMER_LEFT_PENALTY=0.030114
PRIMER_RIGHT_PENALTY=0.051910
PRIMER_LEFT_SEQUENCE=CACTACCGTGTTGTGAAATACGA
PRIMER_RIGHT_SEQUENCE=GTCACCAGTTCATGATGCAGTTA
PRIMER_LEFT=3547,23
PRIMER_RIGHT=4195,23
PRIMER_LEFT_TM=59.970
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=649
PRIMER_PAIR_PENALTY_1=0.0820
PRIMER_LEFT_1_PENALTY=0.030114
PRIMER_RIGHT_1_PENALTY=0.051910
PRIMER_LEFT_1_SEQUENCE=CACTACCGTGTTGTGAAATACGA
PRIMER_RIGHT_1_SEQUENCE=TCACCAGTTCATGATGCAGTTAC
PRIMER_LEFT_1=3547,23
PRIMER_RIGHT_1=4194,23
PRIMER_LEFT_1_TM=59.970
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_1_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_1_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=648
PRIMER_PAIR_PENALTY_2=0.0884
PRIMER_LEFT_2_PENALTY=0.036444
PRIMER_RIGHT_2_PENALTY=0.051910
PRIMER_LEFT_2_SEQUENCE=TGTGAAATACGAGCGGATTAAGT
PRIMER_RIGHT_2_SEQUENCE=TCACCAGTTCATGATGCAGTTAC
PRIMER_LEFT_2=3558,23
PRIMER_RIGHT_2=4194,23
PRIMER_LEFT_2_TM=60.036
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=39.130
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_2_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_2_MISPRIMING_SCORE=24.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=637
PRIMER_PAIR_PENALTY_3=0.0884
PRIMER_LEFT_3_PENALTY=0.036444
PRIMER_RIGHT_3_PENALTY=0.051910
PRIMER_LEFT_3_SEQUENCE=TGTGAAATACGAGCGGATTAAGT
PRIMER_RIGHT_3_SEQUENCE=GTCACCAGTTCATGATGCAGTTA
PRIMER_LEFT_3=3558,23
PRIMER_RIGHT_3=4195,23
PRIMER_LEFT_3_TM=60.036
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=39.130
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_3_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_3_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=638
PRIMER_PAIR_PENALTY_4=0.0927
PRIMER_LEFT_4_PENALTY=0.028502
PRIMER_RIGHT_4_PENALTY=0.064245
PRIMER_LEFT_4_SEQUENCE=GAACCTACGGACAGGTGTACAAG
PRIMER_RIGHT_4_SEQUENCE=TAGACCACTTCTTGGACTTGAGC
PRIMER_LEFT_4=296,23
PRIMER_RIGHT_4=973,23
PRIMER_LEFT_4_TM=59.971
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_4_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_4_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=678
PRIMER_PAIR_PENALTY_5=0.1048
PRIMER_LEFT_5_PENALTY=0.030114
PRIMER_RIGHT_5_PENALTY=0.074695
PRIMER_LEFT_5_SEQUENCE=CACTACCGTGTTGTGAAATACGA
PRIMER_RIGHT_5_SEQUENCE=ATGCAGTTACGGTTCAGAGTCAT
PRIMER_LEFT_5=3547,23
PRIMER_RIGHT_5=4181,23
PRIMER_LEFT_5_TM=59.970
PRIMER_RIGHT_5_TM=60.075
PRIMER_LEFT_5_GC_PERCENT=43.478
PRIMER_RIGHT_5_GC_PERCENT=43.478
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_5_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_5_MISPRIMING_SCORE=21.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_5_END_STABILITY=7.4000
PRIMER_RIGHT_5_END_STABILITY=6.3000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=635
PRIMER_PAIR_PENALTY_6=0.1111
PRIMER_LEFT_6_PENALTY=0.036444
PRIMER_RIGHT_6_PENALTY=0.074695
PRIMER_LEFT_6_SEQUENCE=TGTGAAATACGAGCGGATTAAGT
PRIMER_RIGHT_6_SEQUENCE=ATGCAGTTACGGTTCAGAGTCAT
PRIMER_LEFT_6=3558,23
PRIMER_RIGHT_6=4181,23
PRIMER_LEFT_6_TM=60.036
PRIMER_RIGHT_6_TM=60.075
PRIMER_LEFT_6_GC_PERCENT=39.130
PRIMER_RIGHT_6_GC_PERCENT=43.478
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=4.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_6_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_6_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_6_END_STABILITY=5.7000
PRIMER_RIGHT_6_END_STABILITY=6.3000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_6=624
PRIMER_PAIR_PENALTY_7=0.1602
PRIMER_LEFT_7_PENALTY=0.051910
PRIMER_RIGHT_7_PENALTY=0.108280
PRIMER_LEFT_7_SEQUENCE=GTAACTGCATCATGAACTGGTGA
PRIMER_RIGHT_7_SEQUENCE=AGGATATCCAAGGCCACTACATT
PRIMER_LEFT_7=4172,23
PRIMER_RIGHT_7=4935,23
PRIMER_LEFT_7_TM=60.052
PRIMER_RIGHT_7_TM=60.108
PRIMER_LEFT_7_GC_PERCENT=43.478
PRIMER_RIGHT_7_GC_PERCENT=43.478
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=8.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_7_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_PAIR_7_MISPRIMING_SCORE=21.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_7_END_STABILITY=7.9000
PRIMER_RIGHT_7_END_STABILITY=6.6000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=764
PRIMER_PAIR_PENALTY_8=0.1602
PRIMER_LEFT_8_PENALTY=0.051910
PRIMER_RIGHT_8_PENALTY=0.108280
PRIMER_LEFT_8_SEQUENCE=TAACTGCATCATGAACTGGTGAC
PRIMER_RIGHT_8_SEQUENCE=AGGATATCCAAGGCCACTACATT
PRIMER_LEFT_8=4173,23
PRIMER_RIGHT_8=4935,23
PRIMER_LEFT_8_TM=60.052
PRIMER_RIGHT_8_TM=60.108
PRIMER_LEFT_8_GC_PERCENT=43.478
PRIMER_RIGHT_8_GC_PERCENT=43.478
PRIMER_LEFT_8_SELF_ANY=6.00
PRIMER_RIGHT_8_SELF_ANY=8.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=1.00
PRIMER_LEFT_8_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_8_MISPRIMING_SCORE=12.00, MER1A Nonautonomous DNA transposon
PRIMER_PAIR_8_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_8_END_STABILITY=6.1000
PRIMER_RIGHT_8_END_STABILITY=6.6000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_8=763
PRIMER_PAIR_PENALTY_9=0.1735
PRIMER_LEFT_9_PENALTY=0.064245
PRIMER_RIGHT_9_PENALTY=0.109273
PRIMER_LEFT_9_SEQUENCE=AGAGAACAAGGATGAACAAGCAG
PRIMER_RIGHT_9_SEQUENCE=GCTCTTTCAGCAACACAAAGTCT
PRIMER_LEFT_9=1769,23
PRIMER_RIGHT_9=2617,23
PRIMER_LEFT_9_TM=59.936
PRIMER_RIGHT_9_TM=60.109
PRIMER_LEFT_9_GC_PERCENT=43.478
PRIMER_RIGHT_9_GC_PERCENT=43.478
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_LEFT_9_SELF_END=1.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_9_MISPRIMING_SCORE=12.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_9_MISPRIMING_SCORE=22.00, reverse HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=6.1000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_9=849
PRIMER_PAIR_PENALTY_10=0.1914
PRIMER_LEFT_10_PENALTY=0.030114
PRIMER_RIGHT_10_PENALTY=0.161311
PRIMER_LEFT_10_SEQUENCE=CACTACCGTGTTGTGAAATACGA
PRIMER_RIGHT_10_SEQUENCE=GTCATGAAGTAAACTTGGCTGCT
PRIMER_LEFT_10=3547,23
PRIMER_RIGHT_10=4163,23
PRIMER_LEFT_10_TM=59.970
PRIMER_RIGHT_10_TM=59.839
PRIMER_LEFT_10_GC_PERCENT=43.478
PRIMER_RIGHT_10_GC_PERCENT=43.478
PRIMER_LEFT_10_SELF_ANY=6.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_MISPRIMING_SCORE=10.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_10_MISPRIMING_SCORE=12.00, LTR10 Human DNA sequence 5'-flanking minisatellite PMS32
PRIMER_PAIR_10_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_10_END_STABILITY=7.4000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=3.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=617
PRIMER_PAIR_PENALTY_11=0.1978
PRIMER_LEFT_11_PENALTY=0.036444
PRIMER_RIGHT_11_PENALTY=0.161311
PRIMER_LEFT_11_SEQUENCE=TGTGAAATACGAGCGGATTAAGT
PRIMER_RIGHT_11_SEQUENCE=GTCATGAAGTAAACTTGGCTGCT
PRIMER_LEFT_11=3558,23
PRIMER_RIGHT_11=4163,23
PRIMER_LEFT_11_TM=60.036
PRIMER_RIGHT_11_TM=59.839
PRIMER_LEFT_11_GC_PERCENT=39.130
PRIMER_RIGHT_11_GC_PERCENT=43.478
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=6.00
PRIMER_LEFT_11_SELF_END=2.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_11_MISPRIMING_SCORE=12.00, LTR10 Human DNA sequence 5'-flanking minisatellite PMS32
PRIMER_PAIR_11_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_11_END_STABILITY=5.7000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_11=606
PRIMER_PAIR_PENALTY_12=0.2332
PRIMER_LEFT_12_PENALTY=0.028502
PRIMER_RIGHT_12_PENALTY=0.204708
PRIMER_LEFT_12_SEQUENCE=GAACCTACGGACAGGTGTACAAG
PRIMER_RIGHT_12_SEQUENCE=CGGTGGTGAGAGTACTTTTTCAG
PRIMER_LEFT_12=296,23
PRIMER_RIGHT_12=434,23
PRIMER_LEFT_12_TM=59.971
PRIMER_RIGHT_12_TM=60.205
PRIMER_LEFT_12_GC_PERCENT=52.174
PRIMER_RIGHT_12_GC_PERCENT=47.826
PRIMER_LEFT_12_SELF_ANY=6.00
PRIMER_RIGHT_12_SELF_ANY=6.00
PRIMER_LEFT_12_SELF_END=2.00
PRIMER_RIGHT_12_SELF_END=1.00
PRIMER_LEFT_12_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_12_MISPRIMING_SCORE=12.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_12_MISPRIMING_SCORE=21.00, L1 Human L1 interspersed repetitive sequence - full length copy
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=7.0000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=139
PRIMER_PAIR_PENALTY_13=0.2690
PRIMER_LEFT_13_PENALTY=0.204708
PRIMER_RIGHT_13_PENALTY=0.064245
PRIMER_LEFT_13_SEQUENCE=CTGAAAAAGTACTCTCACCACCG
PRIMER_RIGHT_13_SEQUENCE=TAGACCACTTCTTGGACTTGAGC
PRIMER_LEFT_13=412,23
PRIMER_RIGHT_13=973,23
PRIMER_LEFT_13_TM=60.205
PRIMER_RIGHT_13_TM=59.936
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=47.826
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_RIGHT_13_MISPRIMING_SCORE=12.00, MER34 a consensus
PRIMER_PAIR_13_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_13_END_STABILITY=9.9000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=6.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=562
PRIMER_PAIR_PENALTY_14=0.2695
PRIMER_LEFT_14_PENALTY=0.051910
PRIMER_RIGHT_14_PENALTY=0.217616
PRIMER_LEFT_14_SEQUENCE=GTAACTGCATCATGAACTGGTGA
PRIMER_RIGHT_14_SEQUENCE=GATATCCAAGGCCACTACATTGA
PRIMER_LEFT_14=4172,23
PRIMER_RIGHT_14=4933,23
PRIMER_LEFT_14_TM=60.052
PRIMER_RIGHT_14_TM=60.218
PRIMER_LEFT_14_GC_PERCENT=43.478
PRIMER_RIGHT_14_GC_PERCENT=43.478
PRIMER_LEFT_14_SELF_ANY=6.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_14_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_14_MISPRIMING_SCORE=22.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_14_END_STABILITY=7.9000
PRIMER_RIGHT_14_END_STABILITY=6.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=762
PRIMER_PAIR_PENALTY_15=0.2695
PRIMER_LEFT_15_PENALTY=0.051910
PRIMER_RIGHT_15_PENALTY=0.217616
PRIMER_LEFT_15_SEQUENCE=TAACTGCATCATGAACTGGTGAC
PRIMER_RIGHT_15_SEQUENCE=GATATCCAAGGCCACTACATTGA
PRIMER_LEFT_15=4173,23
PRIMER_RIGHT_15=4933,23
PRIMER_LEFT_15_TM=60.052
PRIMER_RIGHT_15_TM=60.218
PRIMER_LEFT_15_GC_PERCENT=43.478
PRIMER_RIGHT_15_GC_PERCENT=43.478
PRIMER_LEFT_15_SELF_ANY=6.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=1.00
PRIMER_LEFT_15_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_15_MISPRIMING_SCORE=11.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_15_MISPRIMING_SCORE=23.00, reverse MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_15_END_STABILITY=6.1000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=761
PRIMER_PAIR_PENALTY_16=0.3112
PRIMER_LEFT_16_PENALTY=0.028502
PRIMER_RIGHT_16_PENALTY=0.282673
PRIMER_LEFT_16_SEQUENCE=GAACCTACGGACAGGTGTACAAG
PRIMER_RIGHT_16_SEQUENCE=TGAGGATCTCCCTGCAGATATAG
PRIMER_LEFT_16=296,23
PRIMER_RIGHT_16=610,23
PRIMER_LEFT_16_TM=59.971
PRIMER_RIGHT_16_TM=59.717
PRIMER_LEFT_16_GC_PERCENT=52.174
PRIMER_RIGHT_16_GC_PERCENT=47.826
PRIMER_LEFT_16_SELF_ANY=6.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=2.00
PRIMER_RIGHT_16_SELF_END=2.00
PRIMER_LEFT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_16_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_16_MISPRIMING_SCORE=24.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=4.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=315
PRIMER_PAIR_PENALTY_17=0.3219
PRIMER_LEFT_17_PENALTY=0.051910
PRIMER_RIGHT_17_PENALTY=0.269941
PRIMER_LEFT_17_SEQUENCE=TAACTGCATCATGAACTGGTGAC
PRIMER_RIGHT_17_SEQUENCE=GGGTTACTTGCTGCTCACACTAT
PRIMER_LEFT_17=4173,23
PRIMER_RIGHT_17=4883,23
PRIMER_LEFT_17_TM=60.052
PRIMER_RIGHT_17_TM=59.730
PRIMER_LEFT_17_GC_PERCENT=43.478
PRIMER_RIGHT_17_GC_PERCENT=47.826
PRIMER_LEFT_17_SELF_ANY=6.00
PRIMER_RIGHT_17_SELF_ANY=3.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_MISPRIMING_SCORE=12.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_17_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_17_MISPRIMING_SCORE=23.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_17_END_STABILITY=6.1000
PRIMER_RIGHT_17_END_STABILITY=5.3000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=711
PRIMER_PAIR_PENALTY_18=0.3219
PRIMER_LEFT_18_PENALTY=0.051910
PRIMER_RIGHT_18_PENALTY=0.269941
PRIMER_LEFT_18_SEQUENCE=GTAACTGCATCATGAACTGGTGA
PRIMER_RIGHT_18_SEQUENCE=GGGTTACTTGCTGCTCACACTAT
PRIMER_LEFT_18=4172,23
PRIMER_RIGHT_18=4883,23
PRIMER_LEFT_18_TM=60.052
PRIMER_RIGHT_18_TM=59.730
PRIMER_LEFT_18_GC_PERCENT=43.478
PRIMER_RIGHT_18_GC_PERCENT=47.826
PRIMER_LEFT_18_SELF_ANY=6.00
PRIMER_RIGHT_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=2.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_MISPRIMING_SCORE=11.00, MLT2C2 Interspersed repeat MLT2C2 - a consensus
PRIMER_RIGHT_18_MISPRIMING_SCORE=12.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_PAIR_18_MISPRIMING_SCORE=22.00, MLT1e (MLT1e subfamily) - consensus sequence
PRIMER_LEFT_18_END_STABILITY=7.9000
PRIMER_RIGHT_18_END_STABILITY=5.3000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=712
PRIMER_PAIR_PENALTY_19=0.3436
PRIMER_LEFT_19_PENALTY=0.028502
PRIMER_RIGHT_19_PENALTY=0.315112
PRIMER_LEFT_19_SEQUENCE=GAACCTACGGACAGGTGTACAAG
PRIMER_RIGHT_19_SEQUENCE=CCTGCAGATATAGGCGATACAGT
PRIMER_LEFT_19=296,23
PRIMER_RIGHT_19=600,23
PRIMER_LEFT_19_TM=59.971
PRIMER_RIGHT_19_TM=59.685
PRIMER_LEFT_19_GC_PERCENT=52.174
PRIMER_RIGHT_19_GC_PERCENT=47.826
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_MISPRIMING_SCORE=12.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_RIGHT_19_MISPRIMING_SCORE=11.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_PAIR_19_MISPRIMING_SCORE=23.00, HSATI Human satellite I DNA HinfI fragment
PRIMER_LEFT_19_END_STABILITY=6.7000
PRIMER_RIGHT_19_END_STABILITY=6.1000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=305
=